Rapid review for some kinases including kinase A / c / calmodulin dependent kinase

1. Rapid review for Kinases
Prepared by tarig omer

2. Objectives'
• Review intracellular signaling
• PKA
• PKC
• Calmodulin

7. Protein Kinase

9. protein kinase A
• cAMP-dependent protein kinase A
• it utilizes many of cAMP-dependent pathways

12. How PKA work ??

13. GENERALLY G-protein play Obligatory role in
kinases action

17. Mechanisms AND cellular effect of PKA is

22. PKC

27. Mechanisms AND cellular effect of PKA is

28. PKC play important role in platelet activation

30. cont. functions of PKS
• PKC isozymes phosphorylate an abundance of substrates, leading to
both short-term cellular responses such as regulation of membrane
transport and long-term responses such as memory and learning.

31. Current active researches…
•PKC & cancer & CVD pathogenesis….

32. Calmodulin dependent kinases

33. Calmodulin..
• This is the protein mediator of many Ca2-stimulated
enzymatic reactions
• Each of the four Ca2-binding
sites occurs in a helix-loop-helix motif called the EF hand, also found in
many
other Ca2-binding proteins.
• Calmodulin associated with a helical domain (red) of one of the many
enzymes it regulates

34. • Calmodulin can be one of numerous subunits of complex proteins and
is particularly involved in regulating various kinases and
enzymes of cyclic nucleotide generation and degradation

38. Structure of Ca2+/calmodulin complex based on
X-ray diffraction and NMR spectroscopy studies
Molecular Biology of the Cell, 2002

40. Summery !