Anti-Acetyl Lysine antibody (STJ97181): http://www.stjohnslabs.com/acetyl-lysine-antibody-p-98816?filter_name=STJ97181 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

1. Rating:
Specificity:
ANTIBODY CUSTOMER REVIEW
Findings: “Specificity was not rigorously assessed in crude
extracts. TSA treatment enhances the signal.-Branko Latvinik,
Cardiff University.
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Protocol
Gel
Electrophoresis
10% polyacrylamide gel,
constant voltage 160 V, 60 min.
Membrane
Wash
1X TBST wash for 3 times.
Transfer
0.45 nm PVDF membrane, 1
piece of gel (wet transfer),
constant voltage 100 V 60 min.
Secondary
Antibody
Probing
Secondary antibody was added to
the solution of 1X TBST with 5%
skimmed milk powder with
1:10000 dilution ratio, for 1.5 hr.
Blocking 1X TBST with 5% skimmed milk
powder for 2 hr.
Membrane
Wash
1X TBST wash for 3 times.
Primary
Antibody
Probing
primary antibody was added to
the solution of 1X TBST with
5% skimmed milk powder, 4˚C
for overnight.
Visualisation ECL visualization for 5 min (the
visualization time was
determined by actual result. It
was ensured that the
visualization time was identical
for the same antigen).
Model Number STJ97181
Antibody Name Anti-Acetyl Lysine
antibody
Application Western Blot
Species Human
Tissue Extracts from Xenopus
embryos, human iPS
cells.
Dilution 1:1000
Secondary Antibody HRP goat anti-rabbit
Secondary Dilution 1:10000
Fig. In Hela and Jurkat
cell line, there were
target bands detected
by STJ91464.Actin-
beta antibody (Figure:
Lane1: Hela; Lane 2:
Jurkat)..