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Photosynthesis
By azim sir
Yeh cell ke andar hona wala
process hai jisme cell simple
subs ko combine karke large
molecular banata hai and
glucose prepared hota hai by
using CO2, H2O in presence of
light and chlorophyll aur
result me O2 byproduct milta
hai.
Oxidation + Reduction =
Redoxreaction
All Green Plant
Early Experiment
Splitting of water
Ferric Ferrous
Haemoglobin oxyhaemoglobin
Site of Photosynthesis
Plant are green.
leaf
Young stem
fruit
Calyx
Mesophyll
Most common green leaf
Spongy
chloroplast
Lens shape
Thickness 40-600A/ 4-6 nm.
length 4 -6µm
width 1-2 µm
Ribosome
Transmission electron micrograph
Photosynthetic Pigments
Chlorophylls
Absorption Spectrum
O2
O2
O2O2
O2
O2
Photo-Biochemical Process
Pigments- colour
material present
in plant cell.
Yeh pani ke saath soluble
nahi hota lekin organic
solvent ke saath hojata hai
like hexane,methan,
ethanol etc
Carotenoids, Phycobilins
and anthocyanin
Note-Carotonoid bhi
water insoluble but
solvent ke saath
soluble hai.
Absorb Blue- Violet region
CaroteneC40H56
Orange
eg. Β-Carotene
Xanthophyll
Yellow
eg. Lutein
C40H56O2
Oxygenated Carotene
TYPE
Nature of light
Action Spectrum
influence
Absorption
Spectrum
Graph
Action
Spectrum
Graph
Absorption spectrum of
Chlorophyll a and action
Spectrum of
photosynthesis Coincide.
Chlorophyll a shows
Max photosynthetic
activity
Structure of Chlorophyll-a
Chlorophyll-a and b
have similar
Molecular Structure.
Only the difference
between CH3 and
CHO.
Typical chlorophyll molecule
Kite like
Polar
hydrophilic
Lipophilic
Porphyrin head
Phytol tail
Shape-Rhomboid
4 pyrole ring
Central
non ionic
Mg atom
Role of Pigments
i.e. Essential and
Accessory
It can absorb and convert
light energy into
chemical energy
Chl-a
Photo excitation of
Chlorophyll-a
Ground State
e-
light
Chl-a
e-
Excited
Chl-a
e-
ionized
Ionized in 10-9sec
Accepter-1
Accepter-1NADPH2
Non cyclic photo phosphorylation
Cyclic photo phosphorylation
ATP
ATP ATP
Accessory pigments broaden the
absorption Spectrum by
harvesting light from regions
different then that of chl-a.
Protect chl-a by oxidation
Role of Pigments
Pigment systems or
photo system
Core complex
Light harvesting + core complex = Antenna complex or accessory pigment.
Electron carrier
Few
Chl-b
50 Carotenoids
Chl-a
P 650
Chl-a
P 673
Chl-a
P 680
Chl-a
P 700
Reaction
centre(R.C.)
for PS ll
Reaction
centre(R.C.)
for PS ll
Few
Chl-b
50 Carotenoids
Chl-a
P 650
Chl-a
P 673
Thylakoid Space
Thylakoid Space
Light Dependent
Reactions
Dark or light
independent
Light
dependent
Divide into 2 phase
Light
reaction
Grana(thyla
koids or
lamellae)
Dark
reaction
Stroma
Light Reaction or light Dependent Reaction
ADP to ATP
Inorganic
phosphate
Light Energy
Adenosine triphosphate
Energy from photoexcitation of reaction Centre
photophosphorylation
Dr. Daniel Arnon
ATP
NADHPH2
ATP
Light dependent reactions
Non-cyclic
photophosphorylation(Z scheme)
Light Dependent
reaction in plants
Non cyclic
photophosphorylati
on/ Z-scheme
Cyclic
photophosphorylati
on
Non cyclic
photophosphorylati
on/ Z-scheme
Non cyclic
photophosphorylati
on/ Z-scheme
Co.Q
4e-
Pq
4e-
4e-
Cyt.b6
4e-
Cyt.f 4e-
Pc
ADP + iP
ATP
700
4e-
4e-
FRS 4e-
FD
4e-
2NADP-2
2NADP
4e-
Mn,Ca,Cl
Assimilatory
power
2NADPH2
Non cyclic photophosphorylation/ Z-scheme
Occurs in all O2 evolving Autotrophs
Light Dependent Reaction
Cyclic Photophosphorylation
cyclic
photophosphorylation
700
e-
FRS
e-
FD
e-
Cyt.b6
e-
Cyt.f
e-
Pc
ADP + iP
ATP
e-
ADP + iP
ATP
LOW
Electron from FD come back to PS-1.
Light Dependent
Reactions
Chemiosmosis
Hypothesis(Proton
Pump)
+
2-
+ + + ++ + +
+
2-
+ + + ++ + ++ +
ADP + iP ATP ADP + iP ATP
NADPH2
Light independent
reactions
introduction
Do not require direct light or Darkness
Light independent reaction
Light
reaction
Grana(thyla
koids or
lamellae)
Dark
reaction
Stroma
6
6
Path of
CO2
Tracer technique
14CO2 Tracer
14CO2
Chlorella scenedesmus
O2
ATP
NADPH
Light Independent
Reactions
Calvin Cycle/C3
Cycle
Calvin Cycle/C3 Cycle
I Carboxylation
I Carboxylation
6CO2
6 RuBP (5C)
6 Unstable keto acid(6C)
Mg+
RUBISCO 12 3PGA(3C)
Mg++
C3 Cycle
12ATP
12ADP
12 1,3 di PGA(3C)
12 3PGA(3C)
Fructose 1,6 di phosphate(6C)
Glycolytic
reversal
C3 plants and
Photorespiration
C3 Plants
High need CO2 for
Carboxylase
RUBISCO
2 RUDP make 3 PGA
instead of 4 as in C3.
From 2 glycine molecule 1 carbon(25%) is lost as
CO2 and 3 carbon(75%) are recovered as PGA.
C4 plants and Kranz
Anatomy
1965
Alternative method for
CO2 fixation in sugarcane
C.R. Slack
1970
Outlined the entire series of
reaction.
Called
C4 pathway or HSK pathway.
(Hatch and Slack pathway)
Kranz anatomy
Kranz derived from
German- Necklace or
wreath(decoration)
Mesophyll-the inner
tissue of a leaf, containing
many chloroplasts.
pallisade
Spongy
No palisade and
No spongy
Homogenous
mesophyll
Small granal
chloroplast with PEP
Carboxylase and less
stroma
More No.
Bundle
sheath Cell
Around Vascular
Bundles
Bigger Agranal
Chloroplast with
RUBISCO and Only
stroma
Less in No.
Dimorphic
Chloroplast
C4 Pathway
C4 Cycle
Mesophyll
Chloroplast(granal)
Bundle sheath
chloroplast(agranal)
Night time
stomata open
Day time
Stomata closed
Initial CO2 fixation
at night
final CO2 fixation at
C3 cycle during day
To check Water Loss
Independence of light and
Dark reactions
Significance of photosynthesis
interdependent
Photosynthesis process
Manufacture food for all
living organism, oxygen,
Balance CO2 and O2.also
O3.
Biomass-
plant
based
material.
Plant which dies turns
into fossile fuel.
Photosynthesis process ki
waja se hi hame timber
matlab lakdi milti hai
furniture etc ke
liye.cotton milta hai
Economical important product in plant-
Alkaloid which are important in medicine
Use in food spices
Food Masala, tea and mahendi
Factor affecting rate of
photosynthesis
Size, No. of leaf (quantity)
and orientation (shape) and
age of leaf(old or new)
affect photosynthesis
process.
More the this more is the
photosynthesis process.
Amount of
chlorophyll in Cell is
more photosynthesis
process is more.
Agar food ka
transformation fast
hoga to process bhi
fast hoga.
All Environmental
factors simultaneously
affect photosynthesis
Law of limiting
factors
Photosynthesis
Photosynthesis
Photosynthesis

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Photosynthesis