1. Characterizing the response of
Vibrio tubiashii to changes in
environment
Tatyana Marushchak, Christin McLemore, Sam White
Steven Roberts, Ralph Elston
School of Aquatic and Fishery Sciences | University of Washington
11. How do we assess alterations in
Vibrio tubiashii physiology?
12. How do we assess alterations in
Vibrio tubiashii physiology?
changes in expression of genes and proteins
13. How do we assess alterations in
Vibrio tubiashii physiology?
changes in expression of genes and proteins
basic biological information complementing
experimental and environmental studies
14. How do we assess alterations in
Vibrio tubiashii physiology?
RATIONALE?
changes in expression of genes and proteins
basic biological information complementing
experimental and environmental studies
27. Summary
• Chitinase gene expression upregulated when adult
oysters are present
• Vthb (upstream of hemolysin) expression is
elevated at 2hrs, then falls.
• Changes in pH alter expression patterns
• Several virulence associated pathways identified via
protein expression
• polar flagellin, lateral flagellin (motility),
• OMP (survival & host invasion)
28. Future Direction
• Expand the number of targets for gene
expression
• Examine other parameters
• Coordinate with experimental and
environmental sampling - relevance
• Characterize the host response in relation
to pathogen physiology