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MAJOR CHEMICAL COMPONENTS OF THE LIVING ORGANISMS Medical Biochemistry Molecular Principles of Structural Organization of Cells CHEMICAL COMPOSITION OF LIVING ORGANISMS In the human organism there are 70 elements of the Periodic Table, which may be divided in four groups: Macrobiogenic elements (>1%) O, C, N, H, Ca, P. Oligobiogenic elements (0,1-1%) Na, K, Cl, S, Mg, Fe. Microbiogenic elements (<0.01%)>50years 45-50%) the content varies depending on the metabolic processes in the specified tissue (10% in the fat tissue, 65-70% in other tissues, 80% in the blood and kidney) maintained at the necessary level through intake (2 liters/day) or produced in the metabolic processes (0.3 liters/day) Distribution: In the cells (intracellular) Outside the cells extracellular fluids (12-16%), blood plasma (5%), lymph (2%) Within closed cavities (intra-cavity water)(1-3%): cerebrospinal fluid, intraocular, pericardial, synovial fluid etc WATER Functions: Dissolution and stabilization of biological molecules and ions in the fluids of the organisms Heat balance control - uptake, conservation, distribution and release of heat Transport – supply of nutrients and excretion of waste products Mechanical (by hydration) – assistance in maintaining intracellular pressure and shape of cells Structural – forming an interlayer between the polar ends of protein and lipids in biological membranes Synthetic (anabolic) – substrate in the synthesis of biological compounds Hydrolytic (catabolic) – substrate in the bond-breaking processes Energetic (electron donating) The cell functions are dependent on: The total amount of intra and extracellular water Hydration of sub-cellular structures Aqueous microenvironment of macromolecules INORGANIC IONS INORGANIC IONS Functions Bioelectric functions – development of potential difference across the cell membrane (neurons, muscle cells) Osmotic functions – control of osmotic and hydro-osmotic pressure Structural – metal ions constituents of macromolecules (proteins, heme) Regulatory – cations exert regulatory action directly binding to enzymes, influencing their activity and rate of the chemical reactions in the cell, or Indirectly, influencing the hormonal regulation Transport – electrons are transported by the cytochromes, enzymes containing Fe2+ or Fe3+ and Cu2+; oxygen is bound to Fe2+ in the structure of hemoglobin Energetic – inorganic phosphate in ATP Mechanical (support) – Ca and P ions are constituents of bones (strength) Synthetic – complex molecules contain I- (hormones), SO42- (ester sulfuric compounds)
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Chemical composition
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chemical composistion
of the cell
2.
3.
Elements of the
cell
4.
5.
6.
Water molecules
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8.
Organic compound in
the cell Basic element: C, H, O
9.
10.
Carbohydrate Monosaccharide
Disaccharide Polysaccharide
11.
12.
Monosaccharides Glucose
Fructose Galactose
13.
14.
15.
16.
17.
18.
19.
Disaccharide Sucrose
Maltose Lactose
20.
21.
22.
23.
polysaccharides . strach
- plant cell storage . glycogen - animal cell storage . cellulose - cell wall
24.
25.
26.
Fatty acid Glycerol
lipids
27.
List the importance
of lipids
28.
29.
30.
31.
32.
proteins . contain
C, H, O, N, S . smallest unit = amino acid . combine -by peptide bond . dipeptide . polypeptide
33.
List the importance
of proteins:
34.
35.
36.
37.
38.
39.
Polypeptide chain
40.
Various protein structures
e.g.: hormone insulin e.g.: hormone, antibodies, enzyme e.g.: hemoglobin
41.
42.
43.
44.
45.
46.
enzyme
47.
what is enzyme?
48.
49.
50.
student presentation
51.
. proteins .
biological catalyst . speed up biochemical reation
52.
53.
“ ase”
endings: sucrase maltase lactase naming of enzymes Name according to substrate it catalyst A few enzymes are named before : pepsin, trypsin, rennin
54.
55.
56.
57.
58.
59.
60.
61.
Factors affecting the
rate of enzymatic reactions
62.
63.
Rate of enzyme
reaction pH 7 1 14 Pepsin [stomach] Amylase [mouth] Amylase [duodenum]
64.
65.
TEMPT [c] Rate
of enzyme reaction 37 60 0
66.
67.
68.
Design an experiment
: The effect of pH/ temperature on enzymatic activities
69.
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