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TABLE OF CONTENTS
1. Cost Analysis
2.Result and Discussion
3. Characteristic Analysis
5. Future scope
4.Conclusion
6.Application to society
7.References
2
Cost Analysis
3
Material Quantity Rate (Rs.) Amount (Rs.)
Fruit waste 6 Kg - -
Pineapple 2 Kg 80 160
Jaggery 6 kg 55 330
Yeast sample quantity 20 20
Storage 2 90 180
Soap Nuts 2 Kg 75 150
Total
840
Cost Analysis
Results and Discussions:
Sources pH Density(g/ml) Viscosity(centipoise) TDS(ppm)
Sample -1 3.28 1.374 0.9746 2002
Sample – 2 2.18 1.695 1.0035 2740
Sources Falvonoids Saponins Tannins
Sample-1 strong None Mild
Sample-2 strong strong Slightly stronger
Bio-chemical Test
1.Saponins – Foam test 2 ml of sample was added to 6 ml of water
and was shaken vigorously. Formation of foam was seen which did
not get dissolved after addition of dilute HCL
2. Flavonoids – Alkaline reagent test The 2 ml of sample was taken in a test tube and few
drops of dilute 10% NaOH solution were added to it. Then dilute HCl was added to the
solution and yellow colour formed and after addition of base it was changed to colourless.
3.Tannins– Ferric chloride test Dilute 5% ferric chloride (FeCl3) was added to the 2 ml of
the sample and the deep blue colour was noted
4
Expected characterization techniques:
1.Enzyme Activity Test
2.FTIR (Fourier Transform Infrared Spectroscopy)
Expected enzyme activity:
-Protease: 5-15% - Breaks down proteins.
- Lipase: 2-8% - Breaks down fats and oils.
- Amylase: 10-20% - Breaks down carbohydrates.
- Cellulase: 2-8% - Breaks down cellulose.
- Xylanase: 1-5% - Breaks down xylan.
- Pectinase: 1-5% - Breaks down pectin.
- Phytase: 1-3% - Breaks down phytic acid.
5
Anti-Fungal Properties of Bio-Enzyme:
3
DAY-1 DAY-4
6
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bioenzyme studies using experimental .pdf

  • 1. TABLE OF CONTENTS 1. Cost Analysis 2.Result and Discussion 3. Characteristic Analysis 5. Future scope 4.Conclusion 6.Application to society 7.References 2
  • 2. Cost Analysis 3 Material Quantity Rate (Rs.) Amount (Rs.) Fruit waste 6 Kg - - Pineapple 2 Kg 80 160 Jaggery 6 kg 55 330 Yeast sample quantity 20 20 Storage 2 90 180 Soap Nuts 2 Kg 75 150 Total 840 Cost Analysis
  • 3. Results and Discussions: Sources pH Density(g/ml) Viscosity(centipoise) TDS(ppm) Sample -1 3.28 1.374 0.9746 2002 Sample – 2 2.18 1.695 1.0035 2740 Sources Falvonoids Saponins Tannins Sample-1 strong None Mild Sample-2 strong strong Slightly stronger Bio-chemical Test 1.Saponins – Foam test 2 ml of sample was added to 6 ml of water and was shaken vigorously. Formation of foam was seen which did not get dissolved after addition of dilute HCL 2. Flavonoids – Alkaline reagent test The 2 ml of sample was taken in a test tube and few drops of dilute 10% NaOH solution were added to it. Then dilute HCl was added to the solution and yellow colour formed and after addition of base it was changed to colourless. 3.Tannins– Ferric chloride test Dilute 5% ferric chloride (FeCl3) was added to the 2 ml of the sample and the deep blue colour was noted 4
  • 4. Expected characterization techniques: 1.Enzyme Activity Test 2.FTIR (Fourier Transform Infrared Spectroscopy) Expected enzyme activity: -Protease: 5-15% - Breaks down proteins. - Lipase: 2-8% - Breaks down fats and oils. - Amylase: 10-20% - Breaks down carbohydrates. - Cellulase: 2-8% - Breaks down cellulose. - Xylanase: 1-5% - Breaks down xylan. - Pectinase: 1-5% - Breaks down pectin. - Phytase: 1-3% - Breaks down phytic acid. 5
  • 5. Anti-Fungal Properties of Bio-Enzyme: 3 DAY-1 DAY-4 6