The document is a presentation on the Comet Assay given by Aishwaray, an M.Pharm student in their 2nd semester. It was presented to Dr. Saumya Das, an Associate Professor at the NOIDA INSTITUTE OF ENGINEERING AND TECHNOLOGY (PHARMACY INSTITUTE).
This document discusses tier 2 safety pharmacology studies, which assess potential adverse effects on organ systems not addressed by core battery studies. It focuses on methods used to evaluate drug effects on renal and gastrointestinal systems. For renal evaluation, in vivo mammalian models are commonly used to measure glomerular function via clearance tests and tubular function via urine analysis. Hemodynamic effects are also assessed. In vitro, non-mammalian, and in silico models provide additional methods. Gastrointestinal evaluation involves tests like barium meals and pylorus ligation, as well as emerging endoscopic, biomarker, and electrophysiological techniques.
Presentation title – In vivo Carcinogenicity studies.pptxShobhiniChandel
This presentation discusses in vivo carcinogenicity studies, including their principles, methods, and procedures. Carcinogenicity studies involve administering test substances to experimental animals over their lifetimes to observe for evidence of cancer development. The presentation describes the study design, which involves exposing test animals to various doses of substances over their lifetime and monitoring for tumors. Key steps include selecting suitable animal models, administering the test substance via routes similar to human exposure, monitoring animals over their lifetimes, and performing detailed examinations to identify any potential cancers.
This document presents information on genotoxicity studies using the in vitro and in vivo micronucleus assay. It introduces the micronucleus test as a method used in toxicological screening to identify potential genotoxic compounds. The document describes the characteristics, materials, equipment, and procedures for conducting the micronucleus assay on mammalian cells, including growing and treating cells, fixation, staining, and analysis under a fluorescence microscope. References are provided for additional information on the micronucleus assay methodology.
Background
Definitions
Roles and responsibilities
Transport and receipt
Identification, labelling and sampling
Characterisation
Handling and disposal
References
This document presents a procedure for conducting genotoxicity studies using in vitro and in vivo micronucleus assays. It introduces the micronucleus test as a method used in toxicological screening to identify potential genotoxic compounds. The procedure involves growing mammalian cells on cover glasses, treating the cells with test compounds, fixing and staining the cells, and examining them under a fluorescence microscope to detect micronuclei formation indicating DNA damage. References are provided for additional information on micronucleus testing methodology and applications.
This document describes an inhalation toxicity study that involves a micronucleus test to identify potential genotoxic compounds. The study uses mammalian non-cancerous adherent cells that are treated with metallodrugs at IC50 values. The cells are then fixed, stained with acridine orange, and analyzed under a fluorescence microscope to identify any DNA damage or genetic material alterations induced by the metallodrugs. The document provides details on the required materials, equipment, cell preparation procedures, treatment process, fixation and staining protocol, and references for the inhalation toxicity study and micronucleus test.
This document discusses chromosomal aberrations, which are changes to the structure or number of chromosomes. It begins by introducing chromosomes and chromosomal aberrations. It then describes the main types of chromosomal aberrations including numerical (changes in number) and structural aberrations. Common chromosomal disorders like Down syndrome are also discussed. The causes of chromosomal aberrations during cell division are explained. Methods for diagnosing chromosomal aberrations both prenatally and after birth through tests like karyotyping and FISH are summarized.
This document discusses tier 2 safety pharmacology studies, which assess potential adverse effects on organ systems not addressed by core battery studies. It focuses on methods used to evaluate drug effects on renal and gastrointestinal systems. For renal evaluation, in vivo mammalian models are commonly used to measure glomerular function via clearance tests and tubular function via urine analysis. Hemodynamic effects are also assessed. In vitro, non-mammalian, and in silico models provide additional methods. Gastrointestinal evaluation involves tests like barium meals and pylorus ligation, as well as emerging endoscopic, biomarker, and electrophysiological techniques.
Presentation title – In vivo Carcinogenicity studies.pptxShobhiniChandel
This presentation discusses in vivo carcinogenicity studies, including their principles, methods, and procedures. Carcinogenicity studies involve administering test substances to experimental animals over their lifetimes to observe for evidence of cancer development. The presentation describes the study design, which involves exposing test animals to various doses of substances over their lifetime and monitoring for tumors. Key steps include selecting suitable animal models, administering the test substance via routes similar to human exposure, monitoring animals over their lifetimes, and performing detailed examinations to identify any potential cancers.
This document presents information on genotoxicity studies using the in vitro and in vivo micronucleus assay. It introduces the micronucleus test as a method used in toxicological screening to identify potential genotoxic compounds. The document describes the characteristics, materials, equipment, and procedures for conducting the micronucleus assay on mammalian cells, including growing and treating cells, fixation, staining, and analysis under a fluorescence microscope. References are provided for additional information on the micronucleus assay methodology.
Background
Definitions
Roles and responsibilities
Transport and receipt
Identification, labelling and sampling
Characterisation
Handling and disposal
References
This document presents a procedure for conducting genotoxicity studies using in vitro and in vivo micronucleus assays. It introduces the micronucleus test as a method used in toxicological screening to identify potential genotoxic compounds. The procedure involves growing mammalian cells on cover glasses, treating the cells with test compounds, fixing and staining the cells, and examining them under a fluorescence microscope to detect micronuclei formation indicating DNA damage. References are provided for additional information on micronucleus testing methodology and applications.
This document describes an inhalation toxicity study that involves a micronucleus test to identify potential genotoxic compounds. The study uses mammalian non-cancerous adherent cells that are treated with metallodrugs at IC50 values. The cells are then fixed, stained with acridine orange, and analyzed under a fluorescence microscope to identify any DNA damage or genetic material alterations induced by the metallodrugs. The document provides details on the required materials, equipment, cell preparation procedures, treatment process, fixation and staining protocol, and references for the inhalation toxicity study and micronucleus test.
This document discusses chromosomal aberrations, which are changes to the structure or number of chromosomes. It begins by introducing chromosomes and chromosomal aberrations. It then describes the main types of chromosomal aberrations including numerical (changes in number) and structural aberrations. Common chromosomal disorders like Down syndrome are also discussed. The causes of chromosomal aberrations during cell division are explained. Methods for diagnosing chromosomal aberrations both prenatally and after birth through tests like karyotyping and FISH are summarized.
The document is a presentation on the Comet Assay given by Shobhini Chandel, an M.Pharm student at the Noida Institute of Engineering and Technology Pharmacy Institute. It was submitted to Dr. Saumya Das, an Associate Professor at the institute.
Introduction
ZNF structure and classification
ZNF’s molecular functions
Physiological role of ZNFs
Role of ZNFs in diseases
Role of ZNFs in neurodegenerative diseases
Topic – Preclinical study of Immunomodulator ShobhiniChandel
This document presents a preclinical study on an immunomodulator. It discusses in vitro and in vivo methods to test immunomodulatory effects, including inhibition of histamine release from mast cells, neutrophil chemotaxis assays, cell lines, murine models of hypersensitivity and macrophage phagocytosis. In vivo methods involve testing the compound's effects on humoral antibody response, delayed hypersensitivity, and macrophage phagocytosis in rats and mice. The document provides details on procedures and limitations of the in vitro and in vivo testing methods. It concludes with references used in the study.
The document discusses various methods for screening hepatoprotective drugs. It describes in vitro models using primary hepatocyte cultures, hepatic stellate cell cultures, and assays measuring proline hydroxylation inhibition. In vivo models inducing liver injury in rats are also outlined, including models using Long Evans Cinnamon rats, hepatic ischemia, allyl alcohol, carbon tetrachloride, and galactosamine. The goal of these screening methods is to evaluate potential drug candidates for protecting against liver toxicity and damage.
The document discusses agarose gel electrophoresis. It begins with an introduction to electrophoresis and gel electrophoresis, explaining how molecules are separated based on size and charge through an applied electric field in a gel matrix. It then describes the basic components and process of agarose gel electrophoresis, including preparing the agarose gel, loading and running the samples, and visualizing the results to separate DNA fragments. Agarose gel electrophoresis is used to separate nucleic acids like DNA and RNA by size and analyze results like PCR products and DNA molecules.
हिंदी वर्णमाला पीपीटी, hindi alphabet PPT presentation, hindi varnamala PPT, Hindi Varnamala pdf, हिंदी स्वर, हिंदी व्यंजन, sikhiye hindi varnmala, dr. mulla adam ali, hindi language and literature, hindi alphabet with drawing, hindi alphabet pdf, hindi varnamala for childrens, hindi language, hindi varnamala practice for kids, https://www.drmullaadamali.com
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it describes the bony anatomy including the femoral head , acetabulum, labrum . also discusses the capsule , ligaments . muscle that act on the hip joint and the range of motion are outlined. factors affecting hip joint stability and weight transmission through the joint are summarized.
Temple of Asclepius in Thrace. Excavation resultsKrassimira Luka
The temple and the sanctuary around were dedicated to Asklepios Zmidrenus. This name has been known since 1875 when an inscription dedicated to him was discovered in Rome. The inscription is dated in 227 AD and was left by soldiers originating from the city of Philippopolis (modern Plovdiv).
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In this webinar, participants learned how to utilize Generative AI to streamline operations and elevate member engagement. Amazon Web Service experts provided a customer specific use cases and dived into low/no-code tools that are quick and easy to deploy through Amazon Web Service (AWS.)
The document is a presentation on the Comet Assay given by Shobhini Chandel, an M.Pharm student at the Noida Institute of Engineering and Technology Pharmacy Institute. It was submitted to Dr. Saumya Das, an Associate Professor at the institute.
Introduction
ZNF structure and classification
ZNF’s molecular functions
Physiological role of ZNFs
Role of ZNFs in diseases
Role of ZNFs in neurodegenerative diseases
Topic – Preclinical study of Immunomodulator ShobhiniChandel
This document presents a preclinical study on an immunomodulator. It discusses in vitro and in vivo methods to test immunomodulatory effects, including inhibition of histamine release from mast cells, neutrophil chemotaxis assays, cell lines, murine models of hypersensitivity and macrophage phagocytosis. In vivo methods involve testing the compound's effects on humoral antibody response, delayed hypersensitivity, and macrophage phagocytosis in rats and mice. The document provides details on procedures and limitations of the in vitro and in vivo testing methods. It concludes with references used in the study.
The document discusses various methods for screening hepatoprotective drugs. It describes in vitro models using primary hepatocyte cultures, hepatic stellate cell cultures, and assays measuring proline hydroxylation inhibition. In vivo models inducing liver injury in rats are also outlined, including models using Long Evans Cinnamon rats, hepatic ischemia, allyl alcohol, carbon tetrachloride, and galactosamine. The goal of these screening methods is to evaluate potential drug candidates for protecting against liver toxicity and damage.
The document discusses agarose gel electrophoresis. It begins with an introduction to electrophoresis and gel electrophoresis, explaining how molecules are separated based on size and charge through an applied electric field in a gel matrix. It then describes the basic components and process of agarose gel electrophoresis, including preparing the agarose gel, loading and running the samples, and visualizing the results to separate DNA fragments. Agarose gel electrophoresis is used to separate nucleic acids like DNA and RNA by size and analyze results like PCR products and DNA molecules.
हिंदी वर्णमाला पीपीटी, hindi alphabet PPT presentation, hindi varnamala PPT, Hindi Varnamala pdf, हिंदी स्वर, हिंदी व्यंजन, sikhiye hindi varnmala, dr. mulla adam ali, hindi language and literature, hindi alphabet with drawing, hindi alphabet pdf, hindi varnamala for childrens, hindi language, hindi varnamala practice for kids, https://www.drmullaadamali.com
Strategies for Effective Upskilling is a presentation by Chinwendu Peace in a Your Skill Boost Masterclass organisation by the Excellence Foundation for South Sudan on 08th and 09th June 2024 from 1 PM to 3 PM on each day.
it describes the bony anatomy including the femoral head , acetabulum, labrum . also discusses the capsule , ligaments . muscle that act on the hip joint and the range of motion are outlined. factors affecting hip joint stability and weight transmission through the joint are summarized.
Temple of Asclepius in Thrace. Excavation resultsKrassimira Luka
The temple and the sanctuary around were dedicated to Asklepios Zmidrenus. This name has been known since 1875 when an inscription dedicated to him was discovered in Rome. The inscription is dated in 227 AD and was left by soldiers originating from the city of Philippopolis (modern Plovdiv).
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In this webinar, participants learned how to utilize Generative AI to streamline operations and elevate member engagement. Amazon Web Service experts provided a customer specific use cases and dived into low/no-code tools that are quick and easy to deploy through Amazon Web Service (AWS.)
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AISHWARY COMET ASSAY.pptx
1. Comet Assay
NOIDA INSTITUTE OF ENGINEERING AND TECHNOLOGY
(PHARMACY INSTITUTE)
Presented by:
Aishwaray
M.Pharm
2nd semester
Submitted to:
Dr. Saumya Das
Associate Professor
NIET(Pharmacy
Institute)