This document discusses post-fumigation procedures for checking the effectiveness of laboratory fumigation. It outlines the steps to perform an experiment using agar plates to check for any remaining microbial contamination. The plates are incubated in the fumigated lab area for 24-36 hours. Colonies are then counted using colony forming units (CFU) to quantify the microbial load. If growth is limited, the results are positive and the lab is ready for aseptic work. However, more than limited growth would be negative, requiring repeated fumigation to eliminate remaining contaminants. Precautions like wearing protective equipment and sterilizing glassware are also described.

1. PRESENTED BY
SHIVANGI SHRIVASTAVA
M.TECH, IV SEMESTER, SOBT, RGPV, BHOPAL
Post-fumigation

2. Post fumigation is the process perform after fumigation for checking
the contaminants are present or not inside the lab.
Last and very important step of fumigation for potential aseptic
environment of laboratory.

3. 1ST STEP AFTER FUMIGATION
Wear PPE kit before going to the fumigated area.
Check the plates in which chemicals are used for
fumigation, in a way to avoid spillage.
Discard the plates carefully in a Biosafety manner

4. Steps of post fumigation
For checking the fumigation impact we should perform an experiment which shows the variation
in microbial load
For that preparing the agar plates and then incubated the petriplates in fumigated area
After 24-36 hrs, colonies will appear on the plates
For checking microbial load, colony should be count by CFU (Colony forming Unit)

5. POST FUMIGATION CONSEQUENCES
Hinders the expected results of
experiments
Contaminants present can cause harmful effect on
human health
Increases hard work while performing
experiments
Waste of hazardous chemicals, euipments, media
and money

6. EXPERIMENT TO CHECK THE
CONTAMINATION
Autoclave all the
glasswares with media
containing 3.8g Agar and
5 g LB Broth in 100ml
distilled water at 121℃,
15 lbs for 15 min
Pour the media into petri-
dish in aseptic condition
i.e. in laminar hood
Placed the petriplates in
the lab where fumigation
was performed for 24-36
hrs.

7. COLONY COUNT
CFU refer to the number of individual colonies of any microorganism that
grow on a plate of media. This value in turn represents the number of bacteria
capable of replicating as they have formed colonies on the plate. There is a
CFU formula which involves sampling.

8. FOR FUMIGATION
For bacterial interpretation
Less than 10 bacterial colonies is acceptable and there is no need for repeated
fumigation. If more fumigation is required.
For fungal interpretation
More than 2 colonies appear which is not acceptable and fumigation is
required again.

9. After 24-36hrs check the results
If growth of any colony appears with limited amount,
It would show positive results ( means now lab is
ready for aseptic conditions experiments)
If growth of microbial colony appears more than limited
amount, it would show negative results ( means contaminants
still present)

10. For eliminating
the contaminants,
repeat the
fumigation

11. PRECAUTIONS
• PPE kit should be wear before entering the fumigated area.
• Glass-wares used for post fumigation should be sterilised.
• Pouring the media into the petri-plates should be done in laminar
hood.
• UV light should be switched off before using the laminar hood.
• Maintain the other chemicals which should not placed in fumigation
otherwise chances of explosion may occur.

12. THANKYOU