microbiological diagnosis

Diagnostic Testing in the Microbiology Laboratory Jane Wong Public Health Microbiologist September 30, 2003 [email_address]

Topics ,[object Object],[object Object],[object Object],[object Object]

Media and Culture ,[object Object],[object Object]

Direct and Indirect Testing ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Sterile versus Non-sterile Body Sites ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Specimens from Sterile Sites ,[object Object],[object Object]

Specimens from Non-Sterile Sites ,[object Object],[object Object],[object Object]

Sensitivity ,[object Object],[object Object],[object Object],[object Object]

Specificity ,[object Object],[object Object],[object Object]

Documentation ,[object Object],[object Object],[object Object],[object Object]

Specimen ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Appropriate Specimen ,[object Object],[object Object],[object Object]

Collection ,[object Object],[object Object],[object Object]

Transport to Laboratory ,[object Object],[object Object],[object Object]

Inoculation of Media ,[object Object],[object Object],[object Object]

Culture media ,[object Object],[object Object],[object Object],[object Object],[object Object]

Isolation of Individual Bacteria ,[object Object],[object Object],[object Object],[object Object],[object Object]

Growth of Colonies ,[object Object],[object Object],[object Object],[object Object],[object Object]

Mixed Culture of Soil Organisms Containing Bacillus anthracis

Colony “Picking” ,[object Object],[object Object]

Colonies of Bacteria in Pure Culture

Pure Culture of Francisella tularensis Colonies After 72 hours Growth

Pure Culture of Yersinia pestis Colonies on Blood Agar After 48 hours of Growth

Yersinia pestis Colonial Morphology Viewed With Transmitted Light

Confirmation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Gram Stain of Streptococcus sp.

B. anthracis Gram stain showing spores

Gram stain of B. anthracis from broth culture

Examples of Biochemical Tests Left: API 50 Test Above: Antimicrobial Sensitivity Test

Yersinia pestis E-Test (Antimicrobial Sensitivity Test)

Case Study ,[object Object],[object Object],[object Object]

Patient Admitted to Hospital ,[object Object],[object Object]

Blood Bottles Incubated ,[object Object],[object Object],[object Object]

18 Hours of Incubation ,[object Object],[object Object]

Microbiologist Suspects Bacillus anthracis ,[object Object],[object Object],[object Object],[object Object]

Bacillus anthracis India Ink Preparation

Growth on a Blood Agar Plate (Petri Dish) After 18-24 Hours

Motility B. anthracis is non-motile. Other Bacillus species are motile.

Laboratory Cannot Rule Out Bacillus anthracis ,[object Object]

Report ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Molecular Tests ,[object Object],[object Object],[object Object]

The Flip Side! ,[object Object],[object Object],[object Object],[object Object],[object Object]

Other Factors in Personnel Shortage ,[object Object],[object Object],[object Object]

Licensing Applications/Year For Clinical Laboratory Scientist Certification

microbiological diagnosis

Empfohlen

Empfohlen

Weitere ähnliche Inhalte

Was ist angesagt?

Was ist angesagt? (20)

Ähnlich wie microbiological diagnosis

Ähnlich wie microbiological diagnosis (20)

Kürzlich hochgeladen

Kürzlich hochgeladen (20)

microbiological diagnosis