SHAPE Society

1. MRI of Atherosclerotic Plaque inMRI of Atherosclerotic Plaque in
Apo E Ko MiceApo E Ko Mice

2.  Stabilization of atherosclerotic plaque inStabilization of atherosclerotic plaque in
Apo E mice using USPIOApo E mice using USPIO
 For this purpose , first we need to showFor this purpose , first we need to show
these particles inside the plaquethese particles inside the plaque
 Using mice has many advantages: smallerUsing mice has many advantages: smaller
size, similar lesions to human, & costsize, similar lesions to human, & cost
effectivenesseffectiveness

3. Apo E Ko mice As A Model forApo E Ko mice As A Model for
Atherosclerotic PlaqueAtherosclerotic Plaque
 They have the mostThey have the most
atherosclerotic lesionsatherosclerotic lesions
similar to humansimilar to human
atherosclerotic lesions.atherosclerotic lesions.
 The lesions are moreThe lesions are more
common in aortic archcommon in aortic arch
and thoracic aorta.and thoracic aorta.

4. MR images (PDW) of abdominal aorta (arrow) in normalMR images (PDW) of abdominal aorta (arrow) in normal
mouse and ApoE-KO mouse show the difference betweenmouse and ApoE-KO mouse show the difference between
normal and atherosclerotic arteries correlated withnormal and atherosclerotic arteries correlated with
histopathology.histopathology.

5. MR image (PDW) of abdominal aorta (arrow) inMR image (PDW) of abdominal aorta (arrow) in
same normal mouse and same location as in previoussame normal mouse and same location as in previous
Figure. Because of smaller pixel size, signal-to-noiseFigure. Because of smaller pixel size, signal-to-noise
ratio in this image is lower than in Figure 1.ratio in this image is lower than in Figure 1.

6. T1-weighted MR images (magnified) correlated withT1-weighted MR images (magnified) correlated with
histopathological findingshistopathological findings

7. Preliminary informationPreliminary information
 We need to know the best time for applyingWe need to know the best time for applying
hyperthermiahyperthermia
 Serial pathology study of Aorta of USPIOSerial pathology study of Aorta of USPIO
injected APO E mice(with the help of Dr.injected APO E mice(with the help of Dr.
Yeh)Yeh)
 Serial MRI study of Aorta of APO E MiceSerial MRI study of Aorta of APO E Mice
before and after USPIO injectionbefore and after USPIO injection

8. Colloidal carbon iron particles could be observedColloidal carbon iron particles could be observed
immediately under the endothelium, as far as theimmediately under the endothelium, as far as the
adventitia, in a hypertension of 7 days duration, 5 hoursadventitia, in a hypertension of 7 days duration, 5 hours
after the administration of colloidal iron oxide.after the administration of colloidal iron oxide.

9. Prussian blue reactionPrussian blue reaction

10. Non-invasive Stabilization of
Atherosclerotic Plaque
30 mice
20mice USPIO injection 10 without injection
5 days later
15with RF 5without RF 5with RF 5without RF
12 hours later
All animals sacrificed
&
Pathology evaluation for apoptosis

21. MRI of Carotid EndarterectomyMRI of Carotid Endarterectomy
SpecimenSpecimen

22. Apo E mouseApo E mouse

30. GalvestonGalveston4.7 Telsa magnetic resonance4.7 Telsa magnetic resonance
imaging –Dr. Michael Quast,imaging –Dr. Michael Quast,