ATagine you tried to cut and paste your gene of imberest into an expr.pdf

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ATagine you tried to cut and paste your gene of imberest into an expression vector for doning tike the figure above using ECoFV restriction enzyme for both ends of your PCR product and your 5... GA T GATC3 yoctor backbene. EoorVis recognition sequence is: 3... CT A T A G... 5 You canfirmed that ligation was successful and you had creular plasmids for transformalion. Ater transfomstion and selecting for bocteria that survived on Ampiciln plates, you find that your colonies do not express your gene of interest. Explan one of the problems with your cloning that may have cauted your experiment to tal..

ATagine you tried to cut and paste your gene of imberest into an expression vector for doning
tike the figure above using ECoFV restriction enzyme for both ends of your PCR product and
your 5... GA T GATC3 yoctor backbene. EoorVis recognition sequence is: 3... CT A T A G... 5
You canfirmed that ligation was successful and you had creular plasmids for transformalion.
Ater transfomstion and selecting for bocteria that survived on Ampiciln plates, you find that your
colonies do not express your gene of interest. Explan one of the problems with your cloning that
may have cauted your experiment to tal.

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