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HIGH PERFORMANCE(PRESSURE) LIQUID CHROMATOGRAPHY
HPLC ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object]
Four types of high performance liquid chromatography (HPLC):   ,[object Object],[object Object],[object Object],[object Object]
◊  improved performance ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Types of HPLC techniques ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Based on principle of separation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Based on scale of operation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
♠  Dependable analysis of biochemical's ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
HPLC instrumentation comprises: ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
 
 
 
Mobile phase reservoir ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
 
Cross-sectional diagram of a simple single – piston reciprocating pump
Solvent delivery systems ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
u ,[object Object],[object Object]
Guard column ,[object Object]
Analytical column ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Particle nature: ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
b
Column packing   ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
DETECTORS ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Photodiode Array Detector
Flourimetric detector ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
RECORDERS   &   INTEGRATORS ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Selection   of   Column ,[object Object],[object Object],[object Object],[object Object]
APPICATIONS OF HPLC ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Quantitative Analysis ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
 
 
 
 

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HPLC