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6. specific cases of illness are random or likely tory methods and powerful new applications of
linked to a common organism—information that the BioNumeric software used by PulseNet to
is crucial to detect outbreaks and to hone in on analyze and match PFGE patterns.
• Supported efforts to build PulseNet Interna-
their source.
tional. There is, for example, now a direct elec-
As a co-sponsor of PulseNet, APHL served
as a clearinghouse throughout last year’s E. coli tronic link between the national PulseNet
Keeping Foodborne Bacteria at Bay O157:H7 investigation, disseminating informa- Canada database in Winnipeg and the PulseNet
tion via web updates, two national teleconfer- USA database in Atlanta. PulseNet Middle East
ences and the CDC’s PulseNet newsletter. was formed in December 2006, with eight coun-
Among other things, the association provided tries participating. And PulseNet Asia Pacific
time-critical updates on the E. coli O157:H7 now hosts annual training
Throughout the
PFGE protocol and information about where to courses in PFGE.
obtain testing supplies. • Managed contracts with
E. coli 0157:H7
Arresting the Spread fresh bagged spinach—soon confirmed as the the state public health
outbreak, APHL
of Pathogenic E. coli Keeping PulseNet
source of the E. coli O157:H7 bacteria. laboratories in Min-
on the Cutting Edge served as a
In 1993, one of the largest foodborne outbreaks Altogether, 199 people had confirmed cases nesota and North Car-
of E. coli O157:H7 ever recorded in the US un- of outbreak-related illness, with more than half While the spinach outbreak was especially no- olina to develop the next
clearinghouse
generation of PulseNet
folded over a period of 39 days before an alert pe- hospitalized and three killed—far fewer people table, PulseNet detected about a dozen additional
for information.
subtyping technology—
diatrician suspected something was awry. By the than would have been affected had the outbreak outbreaks last year and, if historical trends con-
time authorities identified the source of the bacte- gone unnoticed longer. tinue, is expected to detect roughly the same num- multiple locus variable-
ria, more than 700 people were sick, more than The difference between 1993 and 2006 is ber in 2007. It is able to do so only because of number tandem-repeat analysis (MLVA)—for
Salmonella and Listeria. APHL was also in-
100 hospitalized and at least four were dead. striking. The considerably faster response time ongoing activities to maintain and strengthen the
Fast forward to August 2006, when E. coli last year is attributable to a critical, public network. APHL has been instrumental in these ac- volved in the validation and β-testing of MLVA
O157:H7 again found its way into the food supply; technology for E. coli O157:H7.
health asset—PulseNet—that did not exist in tivities. Last year, the association:
• Organized the 10th anniversary PulseNet meet-
this time, an especially virulent strain of the bac- • Participated in a National Laboratory Training
1993. Initiated by APHL and CDC in 1996,
ing, held over three and a half days last April,
terium. But instead of taking weeks to detect clus- Network course, “Laboratory Investigation of
PulseNet is a national network of public health
ters of infection, public health laboratory in conjunction with the National Foodborne
laboratories that perform a standardized DNA Foodborne Illness.” Public health laboratory
microbiologists suspected an outbreak within “fingerprinting” technique (called pulsed-field Epidemiologists Meeting in Miami, Florida. Sci- scientists from 16 states enrolled in the one-
days of the first cases of illness. Just two weeks gel electrophoresis or PFGE) on organisms asso- entists from every PulseNet member labora- week course, held at the Virginia Division of
after suspicions surfaced, the US Food and Drug ciated with foodborne disease. PulseNet en- tory examined the link between zoonotic and Consolidated Laboratory Services in Rich-
Administration (FDA) advised consumers to avoid foodborne illness, the latest PulseNet labora-
ables scientists to quickly determine whether mond, Virginia.
2006 ANNUAL REPORT
2 APHL 2006 ANNUAL REPORT APHL 2006 ANNUAL REPORT 3
Opposite page: Representation
of a virus of unknown etiology.
member laboratories to serve as faculty for four
Given the availability of multiple influenza assays,
international workshops to train scientists in
scientists must also be aware of the optimal use
real-time, reverse transcription, polymerase
of several specific laboratory procedures.
To assist public health laboratories trying to chain reaction (RT-PCR)—a rapid, molecular tech-
sort through the profusion of information to opti- nique used to detect and subtype influenza
Sustaining Progress in Infectious Disease Control mize their influenza surveillance, APHL convened a viruses. Laboratorians in Brazil, Cambodia, In-
working group of virology experts to develop testing donesia, Nigeria,
If H5N1 arrives
algorithms for use in two different situations: The Philippines,
• Routine surveillance for seasonal influenza Thailand, Uganda
in the US, public
strains during WHO pandemic Phase 3, and Vietnam partici-
health labs will
including recommendations for testing if pated in the
detect it, confirm
novel subtypes appear. APHL/CDC-spon-
• Testing and interpretation for H5N1 if human sored program. (In
its identity and
Keeping Watch for Pandemic Flu
The main impetus for establishing state public cases are confirmed in the US or are sus- September 2006,
track its behavior.
health laboratories a century ago was the detection Throughout much of 2006—and early 2007—the pected after epidemiologic risk assessment. APHL co-hosted a
and control of infectious diseases, and this remains World Health Organization (WHO) designated the The association disseminated these guide- three-day, train-the-
a principal responsibility today. Now, however, the world in influenza pandemic alert Phase 3, charac- lines nationwide and will continue to update them trainers program for potential future work-
US has a considerably larger and more mobile pop- terized by limited human-to-human transmission. as it gathers information about how tests are per- shop faculty.)
ulation to monitor and more complex and rapidly (Phase 6 is the highest alert state.) Experts at the forming, how the virus is changing and whether and • Co-hosted, with the CDC Influenza Branch, a
changing laboratory technology—both of which re- WHO and elsewhere believe that the world is now meeting to assess the role of commercial labora-
how assays are altered.
quire far greater coordination among state, federal closer to another influenza pandemic than at any APHL’s expert guidelines will not only help to tories in pandemic influenza response. Partici-
and global public health laboratory authorities. time since 1968, when the last pandemic occurred. expedite testing and to improve the accuracy of pants included representatives of large
It is APHL’s mission to meet this need by facili- If the pandemic avian influenza H5N1 virus test results, but will also limit indiscriminate test- commercial clinical laboratories, federal health
tating timely information exchange on any number ing for H5N1 to protect the H5N1 reagent supply
arrives in the United States, public health labora- authorities and representatives of the major epi-
of technical topics. The association’s goal is to for a true event.
tory scientists will be the ones to detect it, to con- demiology and laboratory professional associa-
place public health laboratories in the best possible firm its identity and to track its behavior. To do so, In addition, APHL carried out three other tions. As the public health community learned
position to detect the next emerging infectious dis- however, they must have access to the latest in- activities to boost influenza surveillance and during the 2001 anthrax attacks, commercial
ease or the next outbreak of a re-emerging response worldwide:
formation on methods to enhance diagnostic sen- and research laboratories can provide valuable
pathogen, such as mumps. Last year saw its fair • At the request of the CDC Influenza Branch, the
sitivity and to detect evolving strains of the virus, support to public health laboratories during a
share of both. association recruited experts from among its
including Asian and North American lineages. major health event or even a serious scare.
annual report
6 APHL 2006 ANNUAL REPORT APHL 2006 ANNUAL REPORT 7
client
Association of Public Health Laboratories
audience
lab professionals and policy makers
responsibilities
creative direction
MEDIA
7. Welcome Letter Welcome to the 2007 Annual The annual meeting program It offers a waterfront with access to
Meeting of the Association of offers venues for attendees to boat cruises, riverfront boardwalks,
Public Health Laboratories and First connect, exchange ideas and develop fish camps and offshore fishing. For
William Becker, DO, MPH State Environmental Laboratory partnerships. Plenary sessions take those who favor terra firma, check out
APHL President-Elect Conference. In the spirit of our up cross-cutting issues, including the Players Championship Course in
theme, “ColLaboration: Partnerships workforce development, biosafety, Ponte Verda; Jacksonville Landing, a
for a New Day,” I encourage you to information transfer, global health waterfront marketplace with shops
reach out to participants from other and food safety. Breakout sessions— and restaurants; the Jacksonville
disciplines, agencies and jurisdictions, in parallel tracks designated “public Zoological Garden; the amusement
and with newcomers to the meeting health laboratories” and park Adventure Landing; or local
while you are in Jacksonville. And “environmental laboratories”—cover museums and historical sites. Please
don’t forget to visit the exhibit hall to performance standards, laboratory ask at the hotel for more information.
2007 APHL Annual Meeting Planning Committee discuss emerging technologies and accreditation, contingency planning, On behalf of the 2007 APHL
William Becker, DO, MPH, Chair other developments in laboratory public-private partnerships and other Annual Meeting Planning Committee,
Duane Boline, PhD diagnostics with our corporate topics. Please note that all sessions I wish you a stimulating and informative
Ming Chan, PhD partners. You never know when are open to all members. conference with time to enjoy
Alpha Diallo, PhD connections you make here will prove Our host city Jacksonville is a Jacksonville and conversations with
Aloysius Hanson, PhD invaluable later. Our attendees can be thriving port town and one of the fellow attendees.
Oscar Pancorbo, PhD easily identified by their name badge Southeast's major cultural, commercial,
Yvonne Hale Salfinger, MS and ribbons. industrial and financial centers.
ColLABoration—Partnerships for a New Day 2007 APHL Annual Meeting and First State Environmental Laboratory Conference ColLABoration—Partnerships for a New Day 2007 APHL Annual Meeting and First State Environmental Laboratory Conference
Monday June 4
Project Public Health Ready: 8:30am–10:00am cont. Regional Laboratory Response Project and water security
7:30am–8:15am cont.
Emergency Preparedness initiatives.
at the Local Level Maine’s Lessons Learned with Environmental/Chemical
LIMS Implementation and Data Exchange
City Terrace 5
Speaker:
Project Public Health Ready, a collaboration of NACCHO and CDC,
John (Jack) Krueger, ME Health and Environmental Testing
2007 APHL Annual Meeting and
confers the designation of “Public Health Ready” on local health
FINAL PROGRAM Laboratory
departments that have been successfully evaluated for excellence in
emergency preparedness planning, workforce competency eLRN and Water Security
First State Environmental Laboratory Conference development and exercise simulations. Speakers will discuss the Speaker:
assessment process and specific laboratory efforts to prepare for Allan Antley, US EPA, Office of Emergency Management
emergency health threats.
Successes and Challenges at CDC/NCEH
Speakers:
ColLABoration—Partnerships for a New Day for Chemical Data Management and Coordination
Lou Brewer, RN, MPH, Tarrant County Public Health Department of LRN-C Chemical Terrorism Response
Guy Dixon, PhD, Tarrant County Public Health Laboratory Speaker:
Robert Jones, Centers for Disease Control and Prevention
June 3–5, 2007
8:30am–10:00am Environmental Plenary Session
10:00am–10:45am Break in Exhibit Hall
Address by Stanley Meiburg,
Jacksonville, FL US EPA/Managing Environmental and Break Sponsored by Fujirebio Diagnostics
Biomonitoring Data for CT Response Grand Ballroom 5 & Foyer
Grand Ballroom 6–8
10:00am–4:30pm Exhibit Hall Open
(1.5 contact hours for this session)
At the end of this session, participants will be able to: 10:45am–12:15pm Concurrent Sessions
• Describe the unique needs associated with environmental and
Planning for Radiological Emergencies—
chemical LIMS implementation
The View from the Lab
• List projects that enhance EPA regional laboratory support of the
Clearwater
eLRN in emergencies
(1.5 contact hours for this session)
• Discuss how laboratories contribute to the security and protection
This session will review of the basics of radiation and
of public health and the environment
radiation safety, sources of radioactive material, scenarios
Moderator:
involving radioactive material requiring lab support, and
Oscar Pancorbo, PhD, MA Department of Environmental Protection
precautions for managers and methods for protecting lab
Laboratories—Critical to the Nation’s Security and staff and facilities from exposure and contamination. The
Protection of Public Health and the Environment Po-210 poisoning case in Britain will also be covered.
Speaker: At the end of this session, participants will be able to:
Stanley Meiburg, US EPA Liaison to NCEH-ASTDR, CDC • List the elements of a plan for responding to radiological
Managing Environmental and Biomonitoring emergencies
Data for CT Response • Describe basic radiation protection concepts as applied
This session will provide information on data collection, exchange in the public health laboratories
networks, and LIMS implementation as used in the laboratory Speakers:
aspects of EPA compliance data and CDC-related chemical Tony Harrison, CO Department of Public Health and
terrorism/biomonitoring data. Come to compare the views of a state Environment Laboratory
lab that collects data for EPA and CDC response networks; the Marinea Mehrhoff, University Hygienic Laboratory
CDC’s National Center for Environmental Health data collection for a Lynn West, WI State Laboratory of Hygiene
chemical terrorism response; and the EPA, including the eLRN,
8 9
annual conference program
client
Association of Public Health Laboratories
audience
lab professionals and policy makers
responsibilities
creative direction
MEDIA