user guide for diffusion calculation in excel sheet

1. Diffusion – How to Calculate Diffusion Calculation in Excel Sheet
SAGAR KISHOR SAVALE 1
Diffusion: How to Calculate Diffusion Calculation in Excel Sheet
Step 1: Std. Calibrating curve
10 mg drug was dissolved in pH 7.4 Phosphate Buffer, to prepared 100 ug/ml solution
0.2 ml solution is diluted with 10 ml Buffer 2 µg/ml solution
0.4 ml solution is diluted with 10 ml Buffer 4 µg/ml solution
0.6 ml solution is diluted with 10 ml Buffer 6 µg/ml solution
0.8 ml solution is diluted with 10 ml Buffer 8 µg/ml solution
1.0 ml solution is diluted with 10 ml Buffer 10 µg/ml solution
1.2 ml solution is diluted with 10 ml Buffer 12 µg/ml solution
(Above all solutions are 10 µg/ml solution)
Step 2: Take absorbance of all solution and Plot graph in MS Excel.
Step 3: After taking absorbance, to plot graph in Excel, Click Insert Chart Scatter
Select the Graph

2. Diffusion – How to Calculate Diffusion Calculation in Excel Sheet
SAGAR KISHOR SAVALE 2
Step 3: To give Chart Tile or Axis Tile
Absorbance of Prepared
Concentrations (10 µg/ml)
Scatter
Inserts
Chart
Select Graph
Generated Graph
Click here to give chart title and Axis title
Title
Axis Title

3. Diffusion – How to Calculate Diffusion Calculation in Excel Sheet
SAGAR KISHOR SAVALE 3
Step 4: Generate the equation
To select line of the graph write click click Add Trendline
To click Format Trendline
Tic – Display equation on chart
Tic – Display R – Squared value on chart
Generate Equation
Y = mx + c
Where, Y = Absorbance,
m= Slope or gradient,
x = Concentration,
c = Y intercept,
To select line of graph
Write click
Add Trendline

4. Diffusion – How to Calculate Diffusion Calculation in Excel Sheet
SAGAR KISHOR SAVALE 4
To click Format Trendline
This winow is open at write side of the
computer screen
Tic – Display equation on chart
Tic – Dispay R squared on chart

5. Diffusion – How to Calculate Diffusion Calculation in Excel Sheet
SAGAR KISHOR SAVALE 5
Step 5: To Prepared Diffusion sheet
Time
(min.)
Abs
Conc
(µg/ml)
DF
Conc
(µg/ml)
Conc
(mg/ml)
Conc
(mg/0.3ml)
Conc
(mg/10ml)
DR %Diff
0 0 0 0 0 0 0 0 0 0
First Reading is always zero
Time: Time for dissolution is vary dosage from to dosage from.
Abs.: Taking solution after suitable time interval, to take absorbance of this solution, if absorbance is
more than 1, to dilute the solution.
Conc µg/ml: 1. To calculate Conc
µg/ml with the help of Y = mx + c (Equation: std.
calibrating curve) this equation,
2. To convert this equation in x = Y-c / m (Y = Abs.)
DF: dilution factor is indicate number time to dilute the given solution (10, 100 and 1000).
Generate this equation
This winow is open at write side of the
computer screen

6. Diffusion – How to Calculate Diffusion Calculation in Excel Sheet
SAGAR KISHOR SAVALE 6
Conc µg/ml: DF * Conc µg/ml to get Conc
µg/ml.
Conc mg/ml: Conc µg/ml / 1000 to get Conc
mg/ml.
Conc (mg/0.3ml): Conc mg/ml * 0.3 to get Conc
mg/0.3ml.
Conc mg/10ml: Conc mg/ml * 10 to get Conc
mg/10ml.
DR (drug release): to select reading in below the zero in DR column * First reading 0 in
Conc mg/10ml to get DR.
%Diff: (% Diffusion rate): (DR*100) / Labelled Claim of drug to get %Diff.
IMPORTANT NOTE:
1. In every calculation in Excel sheet, “Always starting the calculation with =this sine
(equal to sine)”, if you’re not used this sine calculation was not done.
2. After complete all calculation to press ENTER button to give your reading.
Mr. Sagar Kishor Savale
[Department of Pharmaceutics]
[R. C. Patel Institute of Pharmaceutical Education and Research Shirpur, 425405, MS, India]
avengersagar16@gmail.com
Always starting calculation with = this sine