Presentación sobre la Dinámica del Aparato de Golgi en plantas y su relación con la división celular".

1. Dynamics of the Golgi apparatus in plants
through cell division
The role of microtubules in the maintenance of regular localization and
arrangement of Golgi apparatus in root cells of Triticum aestivum L.
Rafael Diego Macho Reyes (b02marer@uco.es)
Molecular and Cellular Biology of Plants

2. Main points
• The Article.
• Introduction:
• Golgi apparatus in plants. Main features.
• Goal of study.
• Multimedia
• Ultrastructure of Golgi Stacks.
• Localization of Golgi apparatus during the cell cycle of wheat root
cells.
• Localization of Golgi apparatus during inhibition of microtubule
assembly.
Rafael Diego Macho Reyes (b02marer@uco.es)

3. 1. The Article
Rafael Diego Macho Reyes (b02marer@uco.es)

4. Introduction. Golgi apparatus in Plants.
Rafael Diego Macho Reyes (b02marer@uco.es)

5. Ultrastructure of Golgi stacks.
Rafael Diego Macho Reyes (b02marer@uco.es)

6. Introduction. Goal study.
•Study the relationships between
microtubules and Golgi stacks
during the cell division in root cells.
Rafael Diego Macho Reyes (b02marer@uco.es)

7. Multimedia
• How is all this process done?
Rafael Diego Macho Reyes (b02marer@uco.es)

8. Rafael Diego Macho Reyes (b02marer@uco.es)
PROPHASE
PRO-METAPHASE
CYTOKINESIS
TELOPHASE

9. Localization of Golgi apparatus during the cell
cycle of wheat root cells.
Rafael Diego Macho Reyes (b02marer@uco.es)
Interphase
Late G2-Phase
Prophase
Golgi Markers:
58K/FTDC
Antibodies
against tubulin
DAPI

10. Rafael Diego Macho Reyes (b02marer@uco.es)
Localization of Golgi apparatus during inhibition of
microtubule assembly.
Prometaphase
Metaphase
Anaphase
Telophase
DAPI
Golgi
Markers
58K/FTDC
Antibodies
against
tubulin

11. • Golgi apparatus is still working through cell division in order to
produce cell Wall compounds for cytokinesis.
• Golgi stacks divide into vesicles that are transported linked to
microtubule network towards the phragmoplast.
Rafael Diego Macho Reyes (b02marer@uco.es)
Wrap-up

12. References
• Wenzhu Wang∗, Elena Lazareva, Igor Kyreev, Elena Smirnova, “The role of microtubules
in the maintenance of regular localization and arrangement of Golgi apparatus in root
cells of Triticum aestivum L.”, Process Biochemistry 47 (2012) 1545–1551
• Danming Tang and Yanzhuang Wang, “Cell cycle regulation of Golgi membrane
dynamics”, Trends in Cell Biology June 2013, Vol. 23, No. 6.
• José Antonio González Reyes, “Sistema de Endomembranas II”, Biología Molecular y
Celular de Plantas, 4º de Grado en Bioquímica.
• “Inner Life of Cell” (Harvard University)
• “Cytokinesis Animal Plant” (Great Pacific Media)
Rafael Diego Macho Reyes (b02marer@uco.es)