1. The Streptococci With Emphasis on Streptococcus and Enterococcus www.freelivedoctor.com
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11. Lancefield Capillary Precipitation Antibody against a strep group antigen Strep Antigen Extract No Precipitate (Negative Test) Precipitate (Positive Test) Ag-Ab interface Ag-Ab interface Rabbit Anti- serum Rabbit Anti- serum Strep Antigen Extract www.freelivedoctor.com
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13. Preliminary Grouping – NOT on test Streptococcus Enterococcus Lactococcus Aerococcus Leuconostoc Pediococcus Gamella Broth ch tet + - + - + - - + + - - + + + Va S S b S S R R S PYR - a + - b + - - + LAP + + + - - + +/- NaCl - + +/- + +/- +/- - 45 o C +/- + - - - + - Broth=growth in broth; ch=chains; tet=tetrads; Va=vancomycin; PYR= pyrrolidonyl arylamidase; LAP=leucine aminopeptidase; NaCl=growth in 6.5% sodium chloride; Growth at 45 o C a Streptococcus pyogenes is PYR positive b Occasional exception Beta hemolysis +/- - b - - - - - www.freelivedoctor.com
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20. Staph Streak For NVS Specimen streaked For isolated colonies Staph aureus culture Band streak NVS colonies “ Satelliting” the Staph streak www.freelivedoctor.com
25. Presumptive ID – NOT on test Organism GAS GBS Groups C,F,G Entero- coccus Group D Strep Viridans Pneumo- cocci Hemo- lysis a b , , , , Baci- tracin S R b R b NA NA NA NA SXT R R S NA NA NA NA CAMP - + - - - - - PYR + - - + - - - Bile Esculin - - - + + - b - Opto- chin NA NA NA R R R S NaCl - - b - + - - - NA=Not applicable; a Weak beta; b occasional exception www.freelivedoctor.com
![Preliminary Grouping of Gram Positive Cocci ,[object Object],Catalase Rothia Staphylococcus Micrococcus PYR + _ + “ A Disk* chains _ S.pyogenes “ GAS” *A disc contains bacitracin Enterococcus Streptococcus sp & other Group genera + Other Strep Group genera S R _ See “Staph” PP Note: SBA hemolysis as alt to PYR? www.freelivedoctor.com](data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7)