This document describes an optimized protocol that enables the co-detection of cluster of differentiation (CD) surface antigens on fixed, permeabilized neural cell populations through combined flow cytometric analysis of both surface and intracellular antigens. This reveals surface molecule markers of human neuropoiesis.

1. Optimized Protocol
enabling the co-detection of cluster of differentiation (CD) surface antigens on fixed, permeabilized neural cell
populations defined by intracellular staining.
Turaç G, Hindley CJ, Thomas R, Davis JA, et al. (2013) Combined Flow Cytometric Analysis of Surface and Intracellular Antigens
Reveals Surface Molecule Markers of Human Neuropoiesis. PLoS ONE 8(6): e68519. doi:10.1371/journal.pone.0068519
http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068519