Neuromics' is a leader in providing Biopharmas, Academic and Government with CFR compliant 2 and 3-D human primary cell assays, media and supplements for discovery. We also provide antibodies, proteins/growth factors, apoptosis kits and genetic engineering/manipulation tools. We now have FDA registered Virus Transport Media (VTM).

1. CHARACTERIZED, TESTED AND DISCOVERY
READY …FOR RESULTSYOU CANTRUST AND UNDERSTAND

2. OUR CORNERSTONE: HUMAN PRIMARY CELLS AND 3-D
TISSUE MODELS, DEFINED MEDIA, FBS AND VTM
2 and 3-D Cell
Based Assays
>250 peer
Reviewed
Publications
3-DTissue-Like
Models
Primary Human
Neuron,Astrocytes and
Microglia
Primary Human
Endothelial Cells and
Pericytes
Cancer Associated
Fibroblasts andTumor
Cells
Human MSCs and
Derived Cells
Primary Antibodies &
Markers
Apoptosis Kits
GeneTools
Proteins and Growth
Factors
Protein Generation &
Purification
FBS, Media & Culturing
Product
New-VTM forTransport
of COVID-19 Test Samples

3. VIRUS TRANPORT MEDIA (VTM)
Using our high standard FBS, each lot of virus transport media (VTM) is manufactured under strict
guidelines as outlined by the CDC, is sterile, and undergoes quality control before release (CoA and ISO). It has
been assigned an FDA registration number.
VTM is available in both tubes, kits (include sterile swabs), or bulk quantities, allowing us to meet the needs of all types
of buyers.

4. SAMPLE CUSTOMER CELLS DATA AND RELATED PUB
Highlights Researchers using our Human Pericytes, Brain
Microvascular Endothelial Cells (HBMES) and Astrocytes to build a
static 3-D Blood-Brain Barrier Model. Ece Bayir, M. Mert Celtikoglu,
Aylin Sendemira.The use of bacterial cellulose as a basement
membrane improves the plausibility of the static in vitro blood-brain
barrier model. https://doi.org/10.1016/j.ijbiomac.2018.12.257.
Image: Pericytes, HBMECS, and Astrocytes 5 days after culturing. Live cells (green) and dead
cells (red).
The investigators concluded, "Caffeine and sucrose permeability
values obtained from all models were close to literature data and
physiological values."

5. WIDELY USED AND FREQUENTLY PUBLISHED
Sample Publications
Catalog Number: HEC02
Ece Bayir, M. Mert Celtikoglu,Aylin Sendemir. (2018).The use of bacterial cellulose as a basement membrane
improves the plausibility of the static in vitro blood-brain barrier model. International Journal
of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2018.12.257
Shavali Shaik, Bridget Kennis, Shinji Maegawa, Keri Schadler,YangYanwen, Keri Callegari, Rishi R. Lulla, Stewart
Goldman, Javad Nazarian,Veena Rajaram, Jason Fangusaro, andVidya Gopalakrishnan. (2018). REST upregulates
gremlin to modulate diffuse intrinsic pontine glioma vasculature. Oncotarget. 2018 Jan 12; 9(4): 5233–5250. doi:
10.18632/oncotarget.23750
Catalog Number: CAF05
Martin Nurmik, Pit Ullmann, Fabien Rodriguez, Serge Haan, Elisabeth Letellier. In search of definitions: Cancer-
associated fibroblasts and their markers. doi: 10.1002/ijc.32193
Top image: HEC02, HBMECs stained with Claudin-5
Lower image: Human CAFS-Pancreatic Stellates (CAF08) cultured in MSCGro™
supplemented with Neuromics' FBS (FBS001). Courtesy of Emily Rodela, TGEN

6. RESULTSYOU CAN TRUST AND UNDERSTAND
3rd Party Service For Customer Feedback
Neuromics review summary
Sample Reviews:
Ravi D.on BirdEye★★★★★
We have ordered Brain derived Endothelial Cells or BECs.We could not find these in different companies,
particularly from multiple donors. Overall, we are happy with the products.
Olesya P.on BirdEye★★★★★
We bought a large order of FBS from Neuromics. Our experience with their customer service was great.They
offered us the best price and worked with us on any issue issues that came up on our side.
Jiehua Z.on BirdEye★★★★★
We ordered the cells, medium and related culture product. Fast ordering and delivery procedure, and nice
technique support.The cells grew well as described and we can easily expand them.
HBMECs Stained with CD146

7. 3-D CELL-BASED ASSAY SOLUTIONS
 3D Models:
 HUVEC Model
 Left Image, HUVECS growing an
insert
 3-D Blood Brain Barrier Models
 Retinal Angiogenesis Model
 Skin Model
 Extracellular Matrixes
• Engineered hydrogels
optimized to cell types
• Nanofibers
 Coating Solutions
 Alphabiocoat (right image)
increases cell attachment and
growth

8. HUMAN BBB MODEL TEST APPLICATIONS
• Toxicology Assays
 Permeability Studies -Compounds, Oligos and Small Molecules :
 General Procedure
 3D Blood Brain Barrier models are thawed and cultured for 4 days to establish viable barrier as
measured by transendothelial electrical resistance (> 150 Ω x cm2).
 The test compound and fluorescent probe are added and the cultures are returned to incubate for
designated times.
 If assessment of alterations to BBB permeability due to drug treatment is required, fluorescent
dextran conjugate is added to wells
 Media is sampled for quantitation of test articles by UPLC, and for quantification of fluorecent
probe by plate reader
 Apparent permeability is calculated
 Bottom Figure:Alterations in apparent permeability of blood brain barrier due to drug
treatment. Data presented as mean ± SEM x 10-6 cm/s. * Indicates p<0.05 for Fisher’s
LSD t-test comparisons to DMSO controls in a post hoc analysis following two-way
ANOVA. Data Curtsey ofVisokol

9. USING OUR HUMAN CELLS
 Using Neuromics brain endothelial
cells (HEC02), pericytes (HMP104)
and astrocyte (HPM202) –
Customers have constructed their
own BBB model
 TEER Assay BBB Model using
Neuromics’
 HBMVECS,Pericytes and Astrocytes.
https://doi.org/10.1016/j.ijbiomac.201
8.12.257
 Immunofluorescence (IF) staining
results indicate that the expression
of tight and adherence junction
proteins increases on the 5th day of
the cultivation.

10. HUMAN BRAIN ASTROCYTES
 Cat No. HMP202
 Source from non-fetal donors
 Average doubling capability of >10
 Recent publications:
 Yang Wu,Yan Li, Liangqing Zheng, PengchengWang,Yan liu,Yongning Wu,
ZhiYong Gonga (2019).The neurotoxicity of Nε-(carboxymethyl)lysine in
food processing by a study based on animal and organotypic cell
culture. Ecotoxicology and Environmental Safety.
https://doi.org/10.1016/j.ecoenv.2019.110077
 Ece Bayir, M. Mert Celtikoglu,Aylin Sendemir. (2018).The use of bacterial
cellulose as a basement membrane improves the plausibility of the static in
vitro blood-brain barrier model. International Journal
of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2018.12.257
 Top image:Astrocytes stained with GFAP (cat no. SP15101)
 Bottom Image:Astrocytes stained with S100 beta (cat no. GT15160)

11. HUMAN BRAIN ENDOTHELIAL CELLS
 Cat No. HEC02
 Source from non-fetal donors
 Average doubling capability of >15
 Recent publications:
 Hyun-Jung Choi, Na-Eun Kim, Il Kwon, Dukhwan Choi, Jayoung Kim, Ji Hoe Heo.
(2019). Fc-saxatilin inhibitsVEGF-induced permeability by regulating claudin-5
expression in human brain microvascular endothelial cells. Microvascular Research.
DOI: https://doi.org/10.1016/j.mvr.2019.103953
 Larry W. Hunter, Muthuvel Jayachandran andVirginia M. Miller (2019). Sex
differences in the expression of cell adhesion molecules on microvesicles derived
from cultured human brain microvascular endothelial cells treated with
inflammatory and thrombotic stimuli. Biology of Sex Differences 201910:26.
https://doi.org/10.1186/s13293-019-0241-y
 Top image: HBMECS stained with CD146
 Bottom Image: HBMECs stained with Claudin-5

12. MORE ENDOTHELIAL CELLS
 Retinal Endothelial Cells - cat no. HEC09
 Recently publication: A. P. Da Cunha, Q. Zhang, M. Prentiss, X. Q.Wu,V. Kainz,Y.Y.
Xu, J.Vrouvlianis, H. Li, N. Rangaswamy, B. Leehy,T. L. McGee, C. L. Bell, C. E. Bigelow,
V. Kansara, Q. Medley, Q. Huang & H.Y.Wu.The Hierarchy of Proinflammatory
Cytokines in Ocular Inflammation. Current Eye Research,Volume 43, 2018 - Issue 4
Published Online: 04 Dec 2017
 Expandable
 Top image: Retinal Endothelial Cells in Culture
 HUVECs – cat no. HEC01
 Lower Image: HUVECs cultured in collagel hydrogel
 Also available – GFP Expressing HUVECs
 Large Intestine Endothelial Cells, Coronary Artery Endothelial Cells, and
more
 Specially formulized media to support growth

13. CANCER-ASSOCIATED FIBROBLASTS
 CAFs cryopreserved at low passage with high passage capabilities
 Colorectal CAFs – cat no. CAF05
 Top Image: Colorectal CAFS fixed and permeabilized then stained with
50ng/mL Hoechst, and α -SMA (1:100). Pictures were collected by Cytation 3
using DAPI & FITC of CAF05. Colorectal CAFs were positive for α -SMA.
 Pancreatic CAFs – cat no. CAF08
 Melanomia CAFs – CAF09
 Breast CAFs – cat no. CAF06
 Bottom Image: Breast cancer-associated fibroblasts (Cat. No. CAF06) were
plated at 5,000/cm² in a Greiner BioOne T75 flask and maintained in MSC-
GRO low serum, complete medium (Cat. No. SC00B1) in a reduced O2
environment (1%O2, 5%CO2, 90%N2) at 37°C in a humidified chamber.

14. GFP OR RFP EXPRESSING CARCINOMA
CELL LINES
 Gastric Carcinoma 87
 Top image: RFP Expressing Gastric Carcinoma 87 Cells – cat no.TR02-RFP
 Glioblastoma
 Prostate Carcinoma Cells (DU 145)
 Renal Adenocarcinoma Cells (ACHN)
 Bottom image: GFP-Expressing Renal Adenocarcinoma Cells – cat no.TR04-
GFP

15. MESENCHYMAL STEM CELLS
 Cord Blood Derived – cat no. MSC001
 5-10 passages
 Top Graph: MSCs expanded through 15 serial passages. Cells were plated at
5,000/cm² in a T25 flask and maintained in MSC-GRO low serum, complete
medium (cat# SC00B1) in a reduced O₂ environment (1%O₂, 5%CO₂,
90%N₂) at 37°C in a humidified chamber. Cells were detached in Accutase
when cultures reached ~ 80 TO 90% confluence.
 Bone Marrow Derived – cat no. SC00BM1 and MSC001
 Placental Derived – cat no. SC00P1
 Passaged 5 to 10 passages in our Low Serum, Complete Medium (#SC00B1)
 Adipose Derived – cat no. SC00AD1
 Human Adipose derived Mesenchymal Stem cells in culture. Cells were plated
at 5,000/cm² in a T75 flask and maintained in cat# SC00B1 media in a reduced
O₂ environment at 37°C in a humidified chamber.

16. PRIMARY ANTIBODIES AND MARKERS
 Antibodies for many research areas:
 Neuroscience
 Apoptosis
 Cancer
 Angiogenesis
 Diabetes and Obesity
 Immune Response
 Stem Cell Research
 Musculoskeletal Disease
 Image: PGP9.5 (cat no. RA12103) staining of rat DRGs
(Dilution 1:500)

17. Phone: 952-374-616
Fax: 612-677-3976
Email: brett@neuromics.com for orders
rose@neuromics.com for tech questions
We welcome your input, feedback and questions:
Pete Shuster-CEO and Owner
Direct Phone: 612-801-1007
Email: pshuster@neuromics.com
5235 W 74th Street Suite 8
Edina, MN, 55439
www.neuromics.com Find us on
• Get Exclusive Discounts and Special offers
• New Product Updates
• Researcher Insights
• The Latest in Neuroscience News