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Hiv diagnosis

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OmEr HaXhme

how to diagnose hiv in elderly patient.. stepwise guide,, very precise

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DIAGNOSIS • OMER BASHIR HASHMI
HOW TO DETECT • HIV SCREENING TESTS CONFIRMATORY TESTS • • MOLECULAR TESTS
SCREENING TESTS Rapid kit test ELISA (enzyme linked immunosorbant essay) • Most reliable, has 99.9 % sensitivity • Third and fourth generations are being used, detect all antibodies (IgM) CONFIRMATORY TESTS WESTERN blot Specificity 99.9%
• Line immunoassay (LIA) • Immunofluorescence assay (IFA) • MOLECULAR MARKERS • Polymerase chain reaction (PCR) RNA Nucliec acid sequence amplification (NASBA) • • P24 CORE ANTIGEN • CD4 CELLS • Risk of infection increses when count less then 200*10^6/L • Help staging disease and monitring therapy
CONTROL PROGRAM | HEALTH DEPARTMENTHIV AND AIDS: DIAGNOSIS & TESTS HEALTH.PUNJAB.GOV.PK OXFOR HANDBOOK OF CLINICAL MEDICINE EIGHTH EDITION . MURAY LORANG. PAGE 408 en.wikipedia.org

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Hiv diagnosis

  • 2. HOW TO DETECT • HIV SCREENING TESTS CONFIRMATORY TESTS • • MOLECULAR TESTS
  • 3. SCREENING TESTS Rapid kit test ELISA (enzyme linked immunosorbant essay) • Most reliable, has 99.9 % sensitivity • Third and fourth generations are being used, detect all antibodies (IgM) CONFIRMATORY TESTS WESTERN blot Specificity 99.9%
  • 4. • Line immunoassay (LIA) • Immunofluorescence assay (IFA) • MOLECULAR MARKERS • Polymerase chain reaction (PCR) RNA Nucliec acid sequence amplification (NASBA) • • P24 CORE ANTIGEN • CD4 CELLS • Risk of infection increses when count less then 200*10^6/L • Help staging disease and monitring therapy
  • 5. CONTROL PROGRAM | HEALTH DEPARTMENTHIV AND AIDS: DIAGNOSIS & TESTS HEALTH.PUNJAB.GOV.PK OXFOR HANDBOOK OF CLINICAL MEDICINE EIGHTH EDITION . MURAY LORANG. PAGE 408 en.wikipedia.org