2. LEARNING OBJECTIVES
At the end of this session, the student should be able to:
1.Describe what are Streptococci Organisms
2.Classification of Streptococci Organisms
3.Describe role played by Streptococcus mutans in health and disease
3. 1. GENERAL PROPERTIES
• Catalase negative
• Gram +ve
• Spherical or Oval Cocci
• Appears in pairs or chains
• 0.7 to 0.9 um in diameter
• causes a wide variety of diseases (fig. 1)
5. 2. PATHOGENICITY
1. products that destroy the phagocytic cells & ingest them
2. produces enzymes that digest the connective tissue resulting in
extensive tissue destruction
3. can also lyse “Fibrin”
9. 4. CULTURE
• Grow well on blood agar
• There are 3 types of hemolytic reactions produced on blood agar
(fig 4 & 5)
1. α -hemolysis
2. β-hemolysis
3. γ-hemolysis
10. β-hemolysis
wide clear translucent zone
of complete hemolysis around α -hemolysis
the colony narrow zone of partial
hemolysis
γ-hemolysis: no hemolysis occurs
T YP ES OF HEMOLY T IC REAC T IONS
(F IG 4)
11. shorter arrow: α hemolysis longer arrow: β-hemolysis
T YP ES OF HEMOLY T IC REAC T IONS
(F IG 5)
12. 3. SEROLOGY
• Surface Carbohydrate antigens are related to their virulence
• Hence serogrouping termed Lancefield grouping is useful in
identification of the more virulent β-hemolytic species
• 20 Lancefield groups are recognized
14. • originally isolated from human teeth by Clark in 1924
• in 1960 caries were induced experimentally in animals, artificially
infected with strains resembling S.mutans
• cells can loose their coccal morphology and appear as cocco-bacilli
15. • 9 serotypes have been identified (a-h & k)
• Human isolates are : c,e f and k
• S.mutans have been implicated as the primary pathogen in :
1.enamel caries in children & young adults
2.root surface caries in elderly
16. • occupy hard non-shedding surfaces i.e teeth or dentures
• Regularly isolated from dental plaque at carious site, but low
prevalence on sound enamel
• opportunistic pathogens (isolated from cases of infective endocarditis)
• communicate with other mutans
18. contribute to characteristic colonial morphology
Extracellular Polysaccharides
(glucan, mutan, fructan)
glucosyl and fructosyltransferase
Streptococcus mutans
excess sugar
Intracellular Polysaccharides
(glucan, mutan, fructan)
act as carbohydrate reserve, used to produce
acid in absence of available sugar
21. • salivarius prefer mucosal surface especially the tongue
• produce unusual quantity of extracellular fructan
• rarely isolated from diseased sites
22. • S. vestibularis prefers the vestibular mucosa
• no extracellular polysaccharide production
• produces ‘urease’ leading to rise in local pH and hydrogen peroxide
24. The group is differentiated into
• S. constellatus
• S. intermedius
• S. anginosus
25. • readily isolated from dental plaque & from mucosal surface
• cause serious, purulent diseases
• commonly found in abscess of internal organs
• no strain produces polysaccharide from sucrose
28. • Early colonizers of tooth
• produce soluble and insoluble glucans from sucrose
• contribute to plaque formation
• ammonia generation from arginine
29. • S. sanguinis produces a protease that can cleave sIgA
• S. gordonii can bind with salivary amylase enabling the breakdown
of starch
30. References
1. Philip D. Marsh, Michael V Martin, “The Resident Oral Microflora” in Oral Microbiology, 5th Edition,
Churchil Livingstone, 2009, pp 25-35