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Nicole Rivera

This slides contain a recopilation of photos from our trip to El Boque Seco de Guanica.

Umweltschutz Unterhaltung & Humor Technologie

Guanica Dry Forest
Photos
Nicole S. Rivera Espinal

We were receiving a small talk from
Dr. Carlos Ricart

We use hats and everything else that
could protect us from the sun

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The purpose of this experiment is to encounter bacteria from tropical soils that have the capability of producing antibiotic. In order to do so, it was necessary to isolate the bacteria, purify it five times and freeze it at a temperature of -80˚C. After purification, gram staining was performed to classify the bacteria as gram positive or negative. The bacteria were characterized by doing purification of genomic DNA and then, a Polymerase Chain Reaction (PCR) with the primers 16sRNA, 16S_1510R and 16S_8F. An electrophoresis was conducted to see if the PCR process went well. The PCR product was purified and analyzed by using Bioinformatics. Finally, both bacteria’s S15UPRCRISENRE30M01 and S15UPRCRISEAFD30M01A had positive results as antibiotic producers for M.luteus.

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