Tratamiento de arrugas con inyecciones intradérmicas de MADE

2. ti MIDICI"A &IOLOGICA APRILI · GIUGNO 2004
M. De Bellis, N. Frasca
•
•.
TREATMENT OF WRINKLES
AND SKIN SLACKENING USING
THE INTRADERMAL INjECTION
OF ACOMPLEX HOMEOPATHIC
REMEDY (MADE®)
RESULTS OF A COHORT CLINICAL STUDY
ON 681 PATIENTS
SUMMARY
Wrinkles and skin slackening are the
most obvious slgn of the passíng of
time, i.e., 8geing from a mere biotogicat
viewpoint. However, they also reflect
the psycho·neuro-endocrino-immuno­
loglcal (PNEI) vlsion of the human being:
in otller words. the skin is tlle main tar­
get of one's psychological experiences
during the somato-psychic process,
whereas it is the starting polnt of or­
ganic wounds that will eventually be­
come the "soul scar" during the psycho­
somatic proce·ss.
The beauty ot the face has been atways
conneeted with a smooth, glowing and
young skin. Therefore, In order to exor­
cise ageing, our society makes us turn
to Plastie Surgery 01' Aesthetic Medi­
cine, whieh are not often eompletely
sueeessful or do not satisfy the pa­
tients' requlrem..nts fully.
For more than five years, the homeo­
pathle remedy MAOe" (Guna, Milan), has
been an etfective alternative to con­
ventional pharmaeologieal treatment
and can eertalnly be regarded as a ref­
erenee drug In Aesthetic Medicine. The
cohort study hereunder, earried out be­
tween 1998 and 2003 on 681 patients.
has proved the efficacy and good toler­
ability of MAOe- both in preventive and
therapeutle terms, in the homeo­
mesotherapie treatment of skin slaek­
enlng as well as all types of wrlnkles,
espeeially linear perioeular and perlt­
abial wrlnkles, showing the best re·
sults in patients aged between 30-40
years and 40-50 years.
KEY WORDS
SKIN AGEING, WRINKLES, MAOE0,
HOMOTOXICOLOGY

3. A "'E~ICINA &10 OGICA APRllE· GIUGNO 2004
INTRODUCTION
The most significant and obvious sign of
the transilíon from youth to old age is
the appearance of facial wrinkles.
"Beauty" has always been associated
with having young, smooth and glowing
skin . However, apart from external
beauty, the skin also reflects a person's
troubles, anxiety and pain - it is a mir­
ror of the sou l and every w rinkle tells
the story of that person 's experiences in
life.
The skin is the pattern on which the
psycho-neuro-endocrino-immunologi­
cal (PNEI) vision of Medicine is based
and where the psychosomatic signs of
psychological troubles become clearly
visible over the years The skin is also
the starting point for the organic wound
that wi II eventua Ily become the "sou I
scar" (non-acceptance of the se lf) du­
ring the somato-psychic process.
On this basis, we can understand why
nowadays, regardless of one's level of
education, social class or religion, it has
become so important to "exorcise" the
aging process, starting with trying to eli­
minate the problem of w rinkles.
According to the American Society of
Plastic Surgeons
(wwwplasticsurgeryorgl), blepharo­
plasty and face lifts account for Just a
third of the total number of plastic sur­
gery operations requested by Ameri­
canso Even in the non-surgical sector of
Aesthetic Medicine, figures are sti ll im­
pressive Collagen Implants, Hyaluronlc
Acid fillers, Goretex implants, Botulinic
Toxin, mechanical dermal abrasion and
C02 laser skin resurfacing are still the
most common therapeutlc stronghold
for specia Iists.
However, results do not always meet the
patients' high expectations.
For more than five years, the homeopa­
thic remedy MAOE ® (GUNA, Milan),
has been an effective alternative to con­
venti onal pharmacological treatment
In this article, the biological interpreta­
tion of the etiopathogenesis of sk in
aging and wrinkles will be on the basis
of the correct therapeutic rationale for
these problems: by studying the com­
position of MAOE®, 'vve wlll be able to
identify this rationale in its homeophar­
macological structure .
The results of an observation study, car­
ried out between 1998 and 2003 on
681 male and female patients, into the
efficacy of MAOE® in the treatment of
wrinkles and slackening of the face and
neck tissues, will be examined and de­
scribed later on.
SKIN AGING
Chronological aging of the skin is the re­
sult of a mixture of biological, bioche­
mical and molecular events established
by the genetic code of each individual
(chronoaging). This is why not all peo­
pie grow old in the same way and the
skin is not the same in al l mdividuals.
Other environmental chemical and
physical factors contribute to aging, and
these are of varying importance in de­
termining ilS type and severity (photoa­
ging)
In order to fully understand the path o­
genic mechanisms leading to aging of
the skin, we need to analyse and con­
sider the same mechanisms that lead to
general organic aging and examine the
metabolic and structural characteristlcs
of human skin.
We aIso need to consider that chronoa ­
ging and photoaging cou ld have a 51­
gnificant impact on the alteration ofso­
me physiological cutaneous mecha­
nisms, not only as independent events
but also as synergic factors.
The results of skm aging are clearly vi­
sible in 1055 of elasticity and turgidity,
and the appearance of wrinkles It can
be traced back to a slowdown in ce ll
turnover (SYCOTIZATION Process ac­
cording to classic Homeopathy)
with a subsequent reduction in elastic
ti ssue and 1055 of the support structure
(but not j ust the support structure as
maintained by Pi schi nger and Heine)
represented by the subcutaneous, loose
fibrillar connective tissue (dermis) .
SKIN PHYSIOPATHOLOGY AND
THE ROLE OF SUBCUTAIIEOUS
COIlIlECTIVE TlSSUE
The normal physiological process of
chronoaging affects aII the structures of
the tegumental system: at epidermis le­
vel, one can see a reduction in mitoses,
a tendency towards premature keratini­
sati on. the dispersion of Melanocytes,
and a reduction in Langerhans cells.
The basal membrane shows a progres­
sive smoothing with the disappearance
of the epithelial crests and dermal pa­
pillae
The dermi s shows a 1055 of thickness
and thinning out of the vascular sup­
port: the collagen fibres are fragmented;
the elastic fibres are disorganised; the
interstitial substance tends to become
uniform , and there are lower numbers
of fibroblasts, mastocytes and Langer­
hans cells.
But wha t causes these phenomena?
There are two ma in theones:
1) accord ing to the first theory ("pro­
grammaticH
) , the programming of
cel l death lieswithin the genetic co­
de;
2) according to the second theory ("de­
generativeH
), aging is a process that
is dependent on exogenous factors
(especia Ily photoaging for the ski n)
and endogenous factors (horm onal
and immunological factors, for
example) that synergically cause a
series of metabolic failures (Oepo­
sition Phase according to the Table
of Homotoxicosis) and alterations in
molecular syntheses. You need only
to think about w hat happens in par­
ticular areas of the face, such as the
eyelids and some periocular areas ­
here a reduction in collagen type I
synthesis is clearly linked to age, but
ultraviolet radiation, and the subse­
quent produ ction of free radical s,
causes a drastic reduction in elastin
and collagen storage and this pro­
bably affects post-transcription me­
chanisms.

4. WRINKLES
Wrinkles are the most visible evidence
of cutaneous atrophy and are caused by
damage to the collagen and elastic Fi­
bres.
We must remember that, as the skin
does not have its own muscular struc­
tures, it is the contraction oF the muscles
below it that determines its shape. As ti­
me goes by, due to changes in tone and
elasticity, the skin can no longer relax
and the First marks remain etched on the
skin and gradually get worse.
Wrinkles can be divided into the Follo­
wing categories:
Linear wrinkles: these are linked to
facial expressions; at first they are re­
versible and are more common in
women. They mainly appear around
the eyes (crow's Feet), between the
eyes (from Frowning). around the
mouth (vertically on the upper lip or
around the mouth, due to smoking
For example), horizonta lIy on the Fo­
rehead (related to emotions, in par­
ticular to anxiety);
Glyphic wrinkles: these are related
to a greater accentuation oF the or­
dinary cutaneous structure. They ap­
pear on cheeks in particular;
Creases these are caused by pro­
longed Facial expressions (for in­
stance while sleeping);
Wrinkles between the nose and
mouth : these are deep incisures ap­
pearing between the external edge
oF the mouth and the nose wings,
delimited by muscles (mouth orbi­
cular and buccinator muscles).
HOMOTOXICOLOGICAL INTER­
PRETATION OF THE ETIOPATHO­
GEr'lIESIS OF WRINKLES AND SKIN
SLACKENING
According to homotoxicological
physiopathology, wrinkles can be cate­
gorised under Deposition Phase - Im­
pregnation of the Tegumental System
.rll I mil . From a homotoxicological
point-oF-view, it is apparent how wrin­
kles and the slackening oF Face and neck
tissues are caused by changes in the
MATRIX (in Fact, both the Deposition
Phase and the Impregnation Phase are
part of the "Matrix Interstitial Substance
Phases").
The connective tissue has mistakenly
been regarded aSJust a support tissue;
the dermis, which has always been re­
garded as just the tissue on which the
skin lays.
However, Homotoxicology regards the
matrix as a truly specialised organic
structure, the "Basic Regulation
System": all internal and external chan­
ges to our environment aFFect cell me­
chanisms via the interstitial substance.
It is via the matrix that the cells are able
to communicate with the external en­
vironment - the amount oF inFormation
stored in the matrix and passed on to
cells as instructions on their physiolo­
gical functioning is enormous. The ma­
trix is the place where the neurovege­
tative endings unravel and the psycho­
lA loIIOICIN4 510l001e. APRILI· GIUGNO 2004
brillar connective tissue are in the der­
mis, then a subsequent pathological al­
teration will obviously appear.
In fac!. it is essential to keep the dermis
well-drained and metabolically eFfi­
cient in order to keep the skin looking
young .
The dermis is composed oF:
Fibroblasts and Fibrocytes and their
extracellular metabolites
Collagen fibres and elastic Fibres
Glycosaminoglycans (GAGs) and
proteoglycans (PGs)
Blood vessels and nerves
Immunocompetent cells
There are two diFFerent areas in the der­
mis:
1. the papillary dermis, which is su­
perFicial and thin and located near
HOMOTOXICOLOGY
SIX-PHASE TABLE
Simplified table
fll'Uttl
allergle
_di
D"","~lo.n.
-1­ - - - -.
neuro-endocrino-immune informatlon
is conveyed through the neural and en­
docrine substances and cytokines.
We know that correct cell Functioning is
based upon the anatomical and Func­
tional integrity oF the matrix, and ulti ­
mately on its "cleanliness" and its de­
toxiFication levels. An accumulation of
stress factors at this level can lead to a
potential triggering oF a pathological
process. If these changes in the loose fi­
the dermatoepidermalJunction, and
is rich in matrix but poor in collagen
and elastin;
2. the reticular dermis, a thicker area
located between the papillary der­
mis and the subcutaneous adipose
tissue: it is rich in collagen and ela­
stic Fibres, contains lower quantities
oF matrix, and is well vascularised
(blood and Iymph capillaries affe­
rent from the underlying subcuta­

5. ~ MEDIWIA II0lO(;I" APRILE· GIUGNO 2004
neous adipose tissue).
The action of the homeopathic drug
MAOE~ is targeted on these two struc­
tures,
The etiopathogenetic process that leads
to the destructuring of the derm is and,
therefore, to wrinkles is characterized
by a series of connected events 11 111 r li:
- Phase 1: a reduced supply of 02 and
nUlrients to the dermis cel!s caused by
the pol!ution of lhe matrix due to cala­
bol ites produced in cel! turnovers (chro­
noaging) and by undrained toxins (free
radical photoaging), causes a slowdown
of intracellular metabolisms and sub­
sequent enzymatic damage;
- Phase 2: the metabol ie distress of the
fibroblast affects its activity leading to
a drastic reduetion in the incretion of
lhe matrix components (in particular
Hyaluronic acid) and the col!agen and
elastic fibres;
- Phase 3: the connective tissue weave
loses compactness, The lack of glycosa­
minoglycans has a dramatic effect on
skin hydration (Hyaluronic acid is a
highly hydrophilic molecule) and ilS tur­
gidity; the reduced vascularisalion of
the epidermis causes lhe skin to lose its
brightness and normal Iymphatic drai­
nage is slowed down, resulting in a vi­
cious cycle that is difficult to break.
Chronoaging + Photoaging
Enzymatlc damage
Slowdown in cell metabolism
Cell alteration
Tissue destructuring (wrinkle)
wrinkles are primarily a METABOLlC
AlTERATION (a result of suffering aged
cel!s)
Instead, a wrinkle trealment should be
an integrated therapy w here fibroblasts
can begin their synthesizing activily (af­
ter specific organ preparation stimula­
lion) as their proper metabol ic function
has returned as a result of the action of
the coenzyme substrates of the Krebs
cycle and they, therefore, have sufficienl
energy levels to maintain their
neosynthesis activity.
At lhe same time, lhe integrity of lhe
functlonal structure of the dermis
should be maintained by means of con­
tinuous detoxification and drainage,
The homeopathic drug MADE® acts on
lhis "cascade" via the synergic aetion of
its four nuclei of components
IPI( n rwsl ,1
7, INTERMEDIATE CATAlYSTS
(Vitamin C 06, Vitamin B7 06, Vi­
tamin 86 06, Nicotinamid 06, Ac.
Cis aconitum 06, Ac, Fumaricum
06, Ac Alpha-ketoglucaricum 06,
Baryum oxalsuccinicum 06, Na­
trium oxalaceticum 06, Natrium
pyruvicum 08, Magnesium gluco­
nicum 06, Manganum phosphori­
cum 07);
[' B I
If any wrinkle trealmenl is to be reliable
then it must take al! these pathogenic
processes into consideralion and not
just act on one aspect of them .
A therapy that is simply a means of
compensating for a deficiency in Hya­
luronie acid from chronoaging or in
other eomponents of the dermal matrix,
would nollake into accounl the fact that
2, "SUIS" ORGANOTHERAPIES
(Co/lagen suis o8-o30-Funiculus
umbilicalis suis oJO-030-Cutis suis
08-030, Placenta suis 070, Mu­
sculus suis 020, Hepar suis O JO,
Glandula suprarenalis suis O JO),
3, CLASSIC HOMEOPATHIC REMEDIES
(Sulphur 072, Mercurius solubilis
Hahnemanni 020, Calcium fluora­
tum 030, Galium aparine 06, Thu­
ja 06),
4, HOMEOPATHIZED ENZYMES
(Hyaluronidase 08-030)
Through its own components, each o{
the drug's {our nuclei develops a struc­
tural and functional tropism that is spe­
cific to each o{ the steps in the process
o{ the etiopathogenic cascade o{ wrin­
kles PI' T' IRrr: ~ . - -,:
1 - THE NUCLEUS OF
INTERMEDIATE CATALYSTS
Its eleelive target is the milochondrion
and, in particular, the Krebs eycle. It has
a release action on the mechanisms as­
signed to energetic metabolism, via the
enzymatic stimulation induced by ho­
meopathic dilutions
The intermediate calalysts in MADE ®
are therefore vital, as wilhout the relea­
se of the oxidative phosphorylation pro­
cesses and the cells ' renewed produc­
tion of energy, lhe fibroblasts could not
reacl to the prol iferalive trophic stimu­
lation simultaneously induced by the
"Suis" organotherapies.
The core of the nucleus of catalysls is
_-ketoglutaric aCld, a Krebs eyele sub­
strate that acls on the enzyme, _-keto­
glularic-dehydrogenase, which is often
blocked in the initial phases (stil! rever­
sible) of fibroblast degeneration,
Homeopathized vitamins are, of cour­
se, included for their antioxidanl activity
(aclion against photoaging and protec­
tion of the matrix glycosaminoglycans),
Vitamin C was included as il ís an im­
portant cofaetor in the transformation of
Prol ine into Hydroxyprol ine,
The two oligo elements (Magnesium
gluconicum 06, Manganum phospho­
ricum 010) are particularly important
for their catalytic action on col!agen
metabolism.

6. aparine 06; ThU¡a 06
"-.1.
.l.'-J~.
Inhibirion ef
the deslrueluríng
ae/ion 01aulologou
Trophie ae/ion
on skill
and eonneetive
Anlioxidant and eatalylie
ae/ion on eollagen
metabollsm
lA MEDICINA !IOLOGICA APRILE· GIUGNO 2004
'l,f.I::r.'"
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...
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Omna'ge
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suppPrI
2 - THE NUCLEUS OF "SU/S"
ORGANOTHERAP/ES
In accordance with the observations of
Dr. H . H. Reckeweg, "Organotherapy"
with homeopathized organ preparations
is based on the use of pigs as donors.
From a homeopathic point of view, we
can confirm that a pig organ or homeo­
pathized pig tissue is extremely similar
to the human homologous organ and as
a result of this "similarity" (greater than
that of other animal species), the thera­
peutic efficacy of a homeopathized
preparation is even greater
This likeness is particularly apparent at
immunological level.
The result is the marked organotropism
of the " Suis" protein for the human ho­
mologous protein.
Due to pigs' almost completely ineffec­
tive detoxification systems. their tissues
and, therefore. their proteins are parti­
cularly toxic (steatosis degeneration).
One, therefare, creates a protein struc­
ture that has the patential characteristics
af a nasade, plus the specific properties
af arganotropism.
A "Suis" organotherapeutic homeopa­
thic preparation is an organ-specific no­
sode, that, via a subliminal immunolo­
gical mechallism, triggers the immune
response of not only the entire RES
Target sites
of MADE®
components
WAINKLES
CLASSIC
HOMEOPHATY
REMEDIES
Sulphur 012: Mercurius
sol Hahn 020. Calcium
fluoralum 030;Gallum
•
aval! ,ronirlJ>=
Therapeutic strategy
'ORAININ-G DRUGS
....",......' .r­ -'­ ,~J'
MADE ~
'INTCRMEfiIATE CATALYSTS ANO ÓUGOElEMENTs.
'.Vil. C 06/ Vil. 81 06/ Vil. B6 06/ Nlc01inamid 06 / Ac. Cis.'
;-aéOríll. 06/ Ar:. lumaricum 06/ Ac. A-keloglul. 05/ Barlum
,oxalsucc. 06/ NatriulTI oxalac. 06/ Natrium pyruv. 08/
,' ~.~!lf".~I~.ITI.l¡I~nfny.m. [)tl,I.M,,!ng"_nll1T.'Ph.~¡;Jltlo~i.9bH.m DlO.i
tj~.s l ./R.
I

7. LA MEOICINA aIO~OGIC_ APRILE - GIUGNO 2004
,
I A ~
Some examples ot tratment with MACEe. Left side: befare treatment; right side: after treatment.

8. system (hystiocytic macrophagicJ, but
also of the target organ or tissue. We can
confirm that the use of organotherapies
in treatment causes a localized "mi­
croinflammation" , which although, of
course, not clinical (because of the di­
lution), is sufficient to "waken" the func­
tionality of the connective matrix.
Their action is also partly "trophic": the­
se Suis proteins, or some substances
contained in them, are substrates for the
enzyme reactions of protein synthesis
("codifying matrices") The fact that they
are diluted in accordance with the Laws
of enzyme kinetics, makes them per­
form as inductors, speeding up protein
synthesis
The following are of particular interest:
• Collagen suis
Although Collagen suis triggers, with
the immunological mechanism, the
function of the loose fibri llar connecti­
ve tissue of the dermis via a subclmical
inflammatory type process, it also car­
ries out a trophic action by stimulating
the fibroblast to increte autologous col­
lagen.
Therefore, we cannot refer to Coll agen
suis simply as having a supplementa­
tion, and, therefore plastic action, but
real biostimulation.
• Funiculus Umbilicalis suis
It is well-known that this organ prepa­
ration is extremely rich in glycosaml­
noglycans (especially Hyaluronic acid).
When these substances are homeopa­
thically diluted, according to enzyme
kinetics Laws, they act as mductors,
starters, and codifying matrices for the
synthesis of autologous glycosami­
noglycans.
• Musculus suis and Cutis suis
It is easy to guess at the rationale behind
the action of these remedies - they sti­
mulate the function of their respective
targets, performing an anti-degenerati­
ve action and stimulating their tro­
phism.
• Placenta Suis
Some of the Growth Factors contained
in the placenta are of particular interest
- especially FGF (Fibroblast Growth
Factor) that can stimulate the fibroblast
receptors to activate their metabolism;
and EGF (Epidermal Growth Factor), a
polypeptide that acts on the epidermal
metabolism.
Placenta derivatives are also well­
known for their action on microcircula­
tion.
• Hepar Suis
The inclusion of this organotherapy was
necessary for activating the emunctory
drainage of this organ, which is closely
Iinked to the skin, both from an energe­
tic (Traditional Chinese Medicine) and
an ontogenetic point of view.
• Glandula suprarenalis Suis
According to Homotoxicology, the sti­
mulation of the suprarenal gland is the
basis for the treatment of pathologies
that are degenerative or somehow con­
nected with ageing.
3 - THE NUCLEUS OF CLASSIC
HOMEOPATHIC REMEDIES
The main homeopathic polycrests with
recognised anti-degenerative properties
were selected, such as Galium aparine
(essential detoxifying and cleavage ac­
tion on toxins acting on connective tis­
sue metabolism) and Thuja (main re­
medy against dysmetabolic mesenchy­
mal pathologies which are the source of
the pathogenesis of wrinkles)
Sulphur is the most su itable remedy for
the skin (the skin contains large
amounts of Cysteine, a sulphur amino
acid (-SH) The sulphur enzymes are ex­
tremely important for the proper func­
tioning of the tegumental system. Sul­
phur also has an important toxin cen­
trifugation action and, therefore, a drai­
nage action as well
Calcium fluoratum and Mercurius so­
lubilis Hahnemanni act on areas prone
to wrinkles - typical of the "Fluoric"
Homeopathic constitution - where we
can see the "wearing out" of the con­
nective tissue leading to varicose veins,
ptoses and wrinkles. In its pathogenesls,
lA MEDICINA !IOlOGICA APRILE· GIUGNO 2004
Mercurius solubilis Hahnemanni is cha­
racterized by signs of destruction.
4 - THE HOMEOPATHIC
ENZYME NUCLEUS
The inclusion of Homeopathic Hyalu­
ronidase is MAOE" s real innovation.
The 08 and 030 homeopathic dilutions
of this enzyme regulate and limit the
physiological destructuring activity of
the autologous hyaluronidase. As a re­
sult, the interstitial substance is com­
pacted and the wrinkle is reduced.
It is essential to act on the Hyaluronic
acid as a whole and "protect" it as, to all
intents and purposes, it can be regarded
as the true "conductor" of the connec­
tive matrix structure and function - it
packs the main macromolecular com­
ponents of the dermis around itself,
such as collagen, proteoglycans, fibro­
nectin and fibropectins, acting as the
framework.
This remedy is, therefore, a true thera­
peutic unit whose structure provides the
correct homotoxicological strategy for
treating degenerative skin diseases
In order to apply an effective wrinkle
treatmen!, we should assume that wrin­
kles are a metabolic alteration. We can
then understand the therapeutic impor­
tance of the nucleus of intermediate ca­
talysts, the importance of including
"suis" organotherapies and homeopa­
thized Hyaluronidase and why classic
homeopathic remed ies are effective:
.. The physlological metabollc activity
of the fibroblast is re-establ ished as a re­
sult of the enzymatic release promoted
by these substances and this is a funda­
mental condition for the dermis cells to
be able to respond to the stimulus in­
duced by the suis organotherapies via
the neosynthesis of the components of
the Interstitial Substance. The modula­
tion of the degenerative process, con­
trolled by the classic homeopathic re­
medies in the preparation, slows down
the aIteration of the cOllnective stroma,
assisted by the homeopathized hyalu­
ronidase which, by reducing the activity

9. Il MEDICIt.¡f, 510l0GIC~ APRILE· GIUGNO 2004
PHASE4
TAEATMENT
OFAAEAS
PAONETOTHE
APPEAAANCE
OFWAINKLES
CONNECTlVE
TlSSUE DAAINAGE
Conslltul/on
dra/nage and support
Epidermis
Popillory dermis
Corium
Tendinous dermis
Hypodermic vascular plexus
Hypodermis
Subcutaneous cell tissue
¡~~oJl/dant andca/a/ytic ' •
1_ sellon on /he collageñl-.
..:-", 'metabol/sm;·N .:1, ./ ~, . . .::
Prolilmtive lrophlc
andanllgenetlll/ve
sI/mula/ron on ltu!
skillilnd lhe CO/Jnsctivs
T lissul!
Nerve ond ~ubpopillory
vmol plexus
Superficial nervQUS plexus
Fibrous eones
Adipose lobules
Superficíollayer
Subcutoneous vessels
and nerves
Aponeurosis
of the corresponding enzyme, fosters
the slowing down of the degenerative
process and, all things considered, en­
courages the compacting of the dermis.
BIOSTIMULATlON OF THE SKIN.
MATERIALS AIlD IVIETHODS
Both the mesotherapy needle (4 mm.
30G) and the collagen needle (13 mm­
30G, Flf 1.:) can be used for this pro­
cedure. The mesotherapy needle is used
for making classlc intradermal wheals in
accordance with the mesotherapy me­
thod, injecting 0.2-0.3 mi for each
wheal IPIr II IH hlll.
- The collagen needle is used for can­
nulating the wrinkle by moving the
needle gently from left to right while in­
jecting the contents of the syringe
IPKn RI 71.
We recommend treating the whole af­
fected area possibly some acupuncture
points:
LI 18 (Large Intesti ne 18): on the an­
terior margin of the sternocleidoma­
stoideus, on a level with the upper
margin of the thyroid cartilage.
ST 4 (Stomach 4): about 1 cm late­
rally to the corner of the mouth.
ST 5 (Stomach 5) on the vertical li­
ne of the pupil, below the inferior
margin of the orbit.
TH 23 (Triple Heater 23) superio­
rally and posteriorally to the extre­
mity of the eyebrow.
GB 1 (Gall bladder 1) 1 cm laterally
to the external margin of the orbit.
SI 19 (Sma 11 Intestine 19) anterior to
the auricular tragus.
The treatment genera lIy consists of 7-10
treatments carried out once a week,
followed by one or more maintenance
sessions which can be monthly, bi- or
trl-monthly

10. lA !¡DI'IN~ arO~OGICA APRILE· GIUGNO 2004
With homeo-mesotherapic biostimula­
tion, one can see an overall revitali­
zation, a considerable improvement in
tissue tone and a clear easing of the
wrinkles.
The improvement is gradual and, above
all, lasting. The end result of the therapy
is a relaxed and rested face with toned,
glowing skin.
MAOE' can be used both as a treatment
for reducing the signs of aging and as a
preventive treatment for maintaining a
youthful face.
As a result of its specific homeopathic
preparation, the medicine has no col­
lateral effects or contraindications. No
biocompatibility test is therefore nee­
ded
PATIENTS ANO METHOOS
In this study, we evaluated the effecti­
veness of the homeopathic complex re­
medy MADE® in the treatment of wrin­
kles and skin slackening via a series of
subjective and objectlve clinical indi­
cators.
It was an observation multicentric study,
carried out according to the Godd Cli­
nical Practice Rules.
For this study we included 681 patients
of both sexes (578 female - 103 male)
aged between 35 and 75 (female) and
between 40 and 70 (male), divided in­
to different age ranges.
AII patients attend ing the practices of
the doctors taking part in this study ha­
ve been included, without exclusion
criteria.
The period of the study lasted 5 years
from 1998 to 2003
The treatment consisted of 8 sessions on
a weekly basis was carried out. For 50­
me patients the treatment was conti­
nued on the basis of one session a
month after the end of the treatment and
another every 2-3 months.
3.8% of the patients dropped out of the
treatment after the first few sessions, for
reasons that were not dependent on the
programme.
..
THE TUNNELLlNG TECHNIQUE
It is a method involving
the décollement of
thtissue triggering a
small inflammatory
reaction and leading to
the disappearance of
wrinkles.
The method applied involved making li­
near infiltrations, 1 cm apart, which we­
re parallel to the skin surface in the me­
dium and medium-deep dermis, accor­
ding to the mesotherapic technique, or
making infiltrations inside the wrinkles,
according to the tunnelling technique.
1I I!. 71
The resu Its were eva Iuated before and
after the specific treatment via the sub­
jective classiflcation of the visual and
tactile characteristics of the wrinkles
and the slackening of the face and neck
tissues II I!. 'l .
Mild reactions to the treatment were ob­
served in 20 cases (3%) with slight ery­
thema in the inJection site, which dis­
appeared of its own accord after a few
minutes.
DISCUSSION -CONCLUSIONS
This observation multicentric study has
shown that MAOE® is highly effective
and has high levels of tolerability in the
homeo-mesotherapic treatment of all
types of wrinkles and skin relaxation,
especia Ily linear periocu lar and peri la­
bial wrinkles and the revitalisation of
the face and the neck - in Groups A and
B, in particular, there was a considera­
ble reduction in the compromisation of
these 2 areas of facial skin, with the dis­
appearance ofwrinkles; in Groups C, O
and E there was a steady improvement,
with the compromisation of the areas
progressing from being obvious to slight
{groups A rnr.u : ¡ I(.l R] " H. D) and B
II'AII 1IIIe I RI:'" 1(1 11111lll-1I ); Groups
C l' BLl:i (lIGLlH.~ 12. ni TBl( ti!. 0 1(,1,
DU{ilfll..LRCS II F.lTI:IU IUI and EIr
111 F 7 (I·lt,1 J{rs 1h. 171
The best resu Its were observed in fema­
le patients between 30-40 and 40-50
years old (groups A and B): this is par­
ticularly significant as it corresponds to
the results one would expect from a bio­
stimulating therapy, which regards a
young person/adult responder as an in­
dividual who still has an optimum reac­
tion capacity both from a metabolic and
a histologic point of view, and with a
state of connective tissue that has not
yet been compromised
The drug's performance was extremely
good both for the patients (Iow cost, vi­
sible and lasting results, satisfied the re­
quest for biological therapies) and for
the doctors (absence of any risk, ap­
preciable results).
~ This study has proved the practicabi­
lity, tolerability and, above all, the effi­
cacy of MAOE@both in preventive and
therapeutic terms. and it can therefore
be regarded as a reference drug in Ae­
sthetic Medicine. •

11. II MIOICI~~A 810 OGltA APRllf" GIUGNO 2004
Before ~
Alter
o "0- -
Absenl Slighl Obvious
FEMALE PATIENTS - Group A (30-40 years)
Perilabial area
% 100
90
80
70
60
50
40
30
20
10
O
Compromised face skin
I R I
Group B
(female patients ­
40-50 years old),
before (B) and after
(A) therapy; n = 96
8 A
85 90
Absent
(89) (94)
6 3
Slight
(6) (3)
5 3
Obvious
(5) (3)
FEMALE PATIENTS - GROUP 8 (40-50 years)
Perilabial area
% 100
90
80
70
60
50
40
30
20
10
O
Absenl Slighl Obvious
Compromised face skin
FEMALE PATIENTS - Group A (30-40 years)
Periocular area
% 100
90
80
70
50
40
30
20
10
O
- - ­
60 ---1
---'~-i
6 1- -
Absenl Slighl Obvious
Compromised face skin
8 A 8 A B A 8 A
65 73 28 58 61 64 49 50
(68) (76) (29) (60) (64) (67) (51) (52)
19 15 45 23 28 26 34 35
(20) (16) (47) (24) (29) (27) (35) (36)
12 8 23 15 7 6 13 11
(12) (8) (24) (16) (7) (6) (14) (12)
FEMALE PATIENTS - GROUP 8 (40-50 years)
Periocular area
% 100
90 - --- Before
80 Alter
70
60
50
~
40
30 24 4
20
10
O ---'
Absenl Slighl Obvious
Compromised face skin

12. B A B
133 146 48 75 7
Absent
(71) (78) (26) (40) (4)
35 27 110 95 126
Slight
(18) (14) (58) (51) (67)
20 15 30 18 55
Obvious
(11 ) (8) (16) (9) (29)
FEMALE PATlENTS - GROUP e (50-60 years)
Perilabial area
% 100
90 Before­
80 After ~
70
60
50
40
30
20 -_ --­
10
O
Absent Slight Obvious
Compromised face skin
--------------------~
------,,....:::.....---l
--~
16-­
LA MEDICLNA BIOLOGICA APRILE · GIUGNO 2004
'·B .1
Group e
(female patients ­
50-60 years old),
before (B) and after
(DA) therapy; n =
A
10
(5)
133
(71)
45
(23)
100
90
80
70
B A B A 188
2 1 O 1
(1) (1 ) (O) (1)
99 103 99 102
(53) (54) (53) (54)
87 84 89 85
(46) (45) (47) (45)
FEMALE PATIENTS - GROUP e (50-60 years)
Periocular area
~-----------------------
--------------------~---
67 71
60 --------------1
50 - - - - - --1
40 ---~---I
30 - - - - - ­ --1
20 - - - - - ­ - ---1
10 .. .,
O ----'
Absent Slight Obvious
Compromised face skin
r.-'n ti
Group D
(female patients ­
60-70 years old),
before (B) and after
(A) therapy; n = 116
Absent
19
(16)
27
(23)
O
(O)
O
(O)
o
(O)
o
(O)
1
(1)
1
(1)
o
(O)
o
(O)
Slight
52
(45)
45
(39)
55
(48)
66
(57)
52
(45)
66
(57)
57
(49)
59
(51)
57
(49)
62
(53)
Obvious
45
(39)
44
(38)
61
(52)
50
(43)
64
(55)
50
(43)
58
(50)
56
(48)
59
(51)
54
(47)
FEMALE PATIENTS - GROUP D (60-70 years)
Perilabial area Periocular area
% 100 % 100
90 Before- - - - - - - - --­ 90 Before- ­
80 - 80
FEMALE PATIENTS - GROUP D (60-70 years)
After After
70 70 ­
6050 ___60 40 4550
40
30
30
20 ----­ 20
10 -­lO o o
O
4t! I
O - o-"~
Absent Slight Obvious Absent Slight Obvious
Compromised face skin Compromised face skin
h
~( 5

13. LA MIDI[IN~ BIOlOGICA APRllI · GIUGNO 2D04
B A B A B A B A B A
1 II .,
Group e
(female patients ­
50-60 years old),
before (B) and after
(DA) therapy; n =
188
Absent
133
(71)
146
(78)
48
(26)
75
(40)
7
(4)
10
(5)
2
(1)
1
(1)
O
(O)
1
(1)
SlIght
35
(1 8)
27
(14)
110
(58)
95
(51)
126
(67)
133
(71 )
99
(53)
103
(54)
99
(53)
102
(54)
Obvious
20
(11)
15
(8)
30
(16)
18
(9)
55
(29)
45
(23)
87
(46)
84
(4$)
89
(47)
85
(45)
FEMALE PATlENTS - GROUP e (50-60 years) . . . . FEMALE PATIENTS - GROUP e (50-60 years)
% 100
90
00
70
60
50
4030
20
10
O
Perilabial area
% 100
I L Before;- 90
ª
le ~
58 -~
51
40
--26~ 1 - ~, ,
~. ,-"~, .•,, . t-, '. 16
_·...1· . o'> ! 9
--1 ~-:<-- tl,-.­- -'- .~
Absent Slight Obvious
Compromised tace skin
B A B A
19 27 O O
Absent (16) (23) (O) (O)
SlIght
52
(45)
45
(39)
55
(48)
66
(57)
Obvious 45
(39)
44
(38)
61
(52)
50
(43)
FEMALE PATIENTS - GROUP D (60-70 years)
Perilablal area
% 100
90 Befare- ­
80 - Alter
70
60
50
40
30
20
10 - - O O
O
Absent Slight Obvious
Compromised tace skin
5
- 00
70
60
50
4030
20
'"
10 - -4­
O
Absent
I _. _______~~_._----""-
B A B A
O O 1 1
(O) (O) (1) (1)
52 66 57 59
(45) (57) (49) (51 )
64 50 58 56
(55) (43) (50) (48)
Periocular area
Slight Obvious
Compromised tace skin
f-ll b
Group o
(female patients ­
60-70 years old),
before (B) and after
(A) therapy; n =116
O
(O)
O
(O)
57
(49)
62
(53)
59
(51)
54
(47)
~. FEMALE PATIENTS· GROUP D (60-70 years)
Periocular area
% 100
90 Betare'--' - - - - - - - - - - - - ­
80 - Alter
70 ­
60 - - - - -­
50 -----45 -43- ­
40
;g ---..===----J-.10 - -0- -0- ­
O
Absent Slight Obvious
Compromised tace skin

14. l A MEDICINA ¡IOLOeIC
r B ,
Group E
(female patients - >
70 years old),
before (B) and after
(A) therapy; n =103
APRILE · GIUGNO 2004
B
3
Absent
(3)
49
Slight
(48)
51
Obvious
(49)
FEMALE PATIENTS - Group E (>70 years)
Perllablal area
% 100
90 Before.
80 ­ ~- -
Alter
_..
70 ­
60
50
40
30
20 }10 O O
O
Absent Slight
Compromised lace skin
111. H
Group B
(male patients ­
40-50 years old),
before (B) and after
(A) therapy; n =50
Slight
Obvious
BibJió ra h
1. De Bellis M. - Manuale di Omeomesoterapia -
Guna Ed , Milano; 2003.
2. Dipartimento Scientilico Guna - Omotossicolo­
gia in Medicina Estetica "Appunti di Bio-mesote­
rapia"; Guna Ed., Milano; 1998.
3. Dipartimento Scientilico Guna - Quaderm di Cli­
nica Omotossicologica "11 Drenaggio"- Guna Ed.,
Milano: 2000.
4. Di Pietro A.. Di Sante G. - II recupero dell'elastici­
ta e del turgore cutaneo mediante iniezione intra­
dermica di acido ialuronico (Ial-Sistem") con tec­
% 100
90 ­
80 l
70 ­
60
50
40
Obvious
A B A
3
(3)
O
(O)
O
(O)
56
(54)
47
(46)
53
(51 )
44
(43)
56
(54)
50
(49)
30
20
10
O
B A B A B A
O O O O O O
(O) (O) (O) (O) (O) (O)
47 51 47 48 44 44
(46) (49) (46) (47) (43) (43)
56 52 56 55 59 59
(54) (51) (54) (53) (57) (57)
FEMALE PATIENS - GROUP E (>70 years)
Periocular ares
Before
After
O
Absent Slight Obvious
Compromised lace skin
Absent
B
40
(SO)
7
(14)
3
(6)
A B A
43 38 41
(S6) (76) (82)
5 7 5
(10) (14) (10)
2 5 4
(4) (1 0) (S)
nica cross-linked - Estralto da Giornale Italiano di
Dermatologia e Venereologia vol. 136-N.3/187-194
(Giugno 2001 ) . Edizioni Minerva Medica - Torino
5 Duprat H. - Materia Medica Omeopatica - Fratelli
Palombl Edltori. Roma; 1983.
6. Reckeweg H.H. - Materia Medica Omeopatica -
Guna Ed., Milano; 1990.
7 Stevens A. , Lowe J. - Istologia Umana ­
Casa Editrice Ambrosiana. Milano; 2003.
8. Seutemann S.. Kastner R. - Omeopatia con I blo­
catalizzatori - Guna Ed.. Milano ; 1991 .
B A B A B A
lS
(36)
21
(42)
38
(76)
40
(80)
36
(72)
39
(78)
22
(44)
20
(40)
10
(20)
10
(20)
12
(24)
10
(20)
10
(20)
9
(1S)
2
(4)
O
(O)
2
(4)
1
(2)

15. LA ~I,DICIN_ ~1¡)LOGiC, APRIL¡ · GIUGNO 2004
1 ·1~ 1
Group C
(ma1e patients ­
50-60 years old),
before (B) and after
(A) therapy; n = 43
B A B A B A B A B A
25 25 22 25 15 15 24 26 24 24
Absent (59) (59) (51) (58) (35) (35) (56) (60) (56) (56)
15 16 14 12 20 21 16 14 16 17
Slight (34) (37) (33) (28) (46) (49) (37) (33) (37) (39)
3 2 7 6 8 7 3 3 3 2
Obvious
(7) (4) (16) (14) (19) (16) (7) (7) (7) (5)
fAB. 1U
Group O
(male patients ­
60-70 years old),
before (B) and after
(A) therapy; n = 10
B A B A B A B A B A
2 2 2 3 1 1 1 1 3 3
Absent (20) (20) (20) (30) (10) (10) (10) (10) (30) (30)
5 6 5 5 6 7 5 6 5 6
Slight (50) (60) (50) (50) (60) (70) (50) (60) (50) (60)
3 2 3 3 3 2 4 3 2 1
Obvious
(30) (20) (30) (30) (30) (20) (40) (30) (20) (10)
Doctor's
O(O) [1 0(0) O(O) 211 (31) 470 (69)
evaluation
I i
---t:, lr DE BELLlS M. - Terapia delle rughe e
Patlent's
I
O(O) O(O) O(O) 191 (28) 490 (72) del rilassamento cutaneo con inie­
evaluatlon
zioni intradermiche di un farmaco
omeopatico complesso (MADE") ­
11.1) II Global evaluation on the treatment: results
Risultati di uno studio multicentrico
su 681 pazienti.
La Med. Biol. 2004/2; 7-19.
-_-~liIiMDm:tIf!-'
Doctor's Dr_ Massimo De BellisO(O) O(O) O(O) 7 (1) 674 (99)
evaluation - Specialista in
Idroclimatologia Medica
Patient's O(O) O(O) O(O) 20 (3) 661 (97)
I
Via Cesare da Sesto, 15
evaluation
I - 20123 Milano
r f: Global evaluation on tolerability