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Thesis ppt
- 1. Involvement of SOS response and C-di-GMP signaling in biofilm formation of Pseudomonas aeruginosa via DNA and Protein release. Supervising Professors Dr. Tao Wei, Chair Dr. Neal Guentzel Dr. Jilani Chaudry Nagarjun Kasaraneni, B.Tech
- 5. Biofilm Matrix-enclosed bacterial populations adherent to each other and/or to surfaces Singh et al . (2002) Nature 417: 552
- 6. Developmental cycle of a biofilm J.W.Costerton et al., Annu. Rev. Microbiol, 1995
- 7. Strategies to prevent biofilm formation Rickard et al. (2003) TIM 11: 94 .
- 9. Riboncleiotide reductase & Hydroxyurea Riboncleiotide reductase (RNR) Cysteine thiyl radical attacks OH-C3’ leading to removal of hydroxide ion of C-2 ’ NTPs dNTPs
- 11. Mechanism of SOS response Bianco and Kowalczykowski, 1998
- 13. Cyclic-di-GMP signaling proteins in different bacteria Simm etal., Mol.Microbiol, 2004
- 14. Aminoglycoside response regulator (Arr) Hoffman et al., Nature 436, 1171, 2005
- 16. Biofilm stimulation is RecA dependent Hydroxyurea –inducible biofilm formation appears RecA-dependent .
- 17. SOS involvement in biofilm stimulation
- 19. DNA release in response to hydroxyurea Whether the release of DNA is under SOS control? [DNA μ g/ml]/cell
- 20. SOS controlled DNA release
- 22. Protein release in response to hydroxyurea 60 kDa : Chaperonin GroEL 30 kDa : Elastase elsB 47 kDa : Elongation factor-TU 1: Cell lysate proteins HU- 2: Cell lysate proteins HU+ 3: Wt -HU Supernatent proteins 4: Wt+HU Supernatent proteins 5: RecA –HU supernatent proteins 6: RecA +HU Supernatent proteins 7: RecA Comp –HU Supernatent proteins 8: RecA Comp +HU Supernatent proteins.
- 25. Correlation of protein release with biofilm stimulation
- 27. Complementation of the recA mutant biofilm with wild type proteins
- 29. Protein release is regulated by Arr Summary From the Protein concentrations and SDS gel profiles suggests the Protein release in response to hydroxyurea appears to be regulated by Arr Wt arr 1 : Wt –HU Supernatent proteins 2 : Wt+HU Supernatent proteins 3 : arr –HU Supernatent proteins 4 : arr +HU Supernatent proteins Extracted at 8 hr time period.
- 33. Thank you
- 34. Cynthia j.Kenyon. , Biomedical press, 1983 Cartoon of the SOS response
Hinweis der Redaktion
- Ribonucleotide reductase (RNR) is an enzyme that controls the cellular concentration of deoxyribonucleotides . Biosynthesis begins with the building up of essential molecules that RNR processes in a catalyzed reaction to make deoxyribonucleotides. RNR assembles deoxyribonucleotides for the synthesis of DNA . The processes are identical in all living organisms. What makes RNR unique from other enzymes is the need for a free radical . Hydroxyurea is a scavenger of free radicals.