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Enzymes: Temperature, pH, andSpecificity Hands-on labs, inc. Version 42-0054-00-01 Review the safety materials and wear goggles when working with chemicals. Read the entire exercise before you begin. Take time to organize the materials you will need and set aside a safe work space in which to complete the exercise. Experiment Summary: Students will learn how enzymes act as catalysts to lower the activation energy required for a reaction to proceed in both catabolic and anabolic processes. Students will study competitive inhibition, negative feedback, and enzyme specificity using lactase on various substrates. They will study the effects of temperature and pH on the ability of lactase to break down lactose. © Hands-On Labs, Inc. www.LabPaq.com 125 ExpErimEnt ObjEctivEs ● To learn how enzymes work ● To understand enzyme specificity ● To relate enzyme activity to temperature, pH, and concentration ● To understand the role of enzymes in digestion of lactose TimeAllocation: 3 hours Safety: So that you understand the proper procedures to use when handling, pouring, heating, etc. chemicals, if you have not already done so, read the section in the introduction to this Lab Manual titled “Laboratory Equipment and Techniques” before beginning this experiment. Also, always wear goggles when handling chemicals, and also use gloves when directed to do so. www.LabPaq.com 126 ©Hands-On Labs, Inc. Experiment EnzymEs TEmpEraTurE pH spEcificiTy matErials MATERiAlS FRoM: lABEl oR BoX/ BAg: QTy iTEM DESCRiPTioN: Student Provides 10 1 Bathroom or facial tissues Freezer 1 10 Milk, 1 cup Paper, tissue in sheets 1 Oven mitt 1 Scissors 2 Spoons 1 1 1 Sucrose (Table sugar), 2.5 grams Stove and pan for Hot Water Bath Water, distilled (1 liter) From LabPaq 1 Glucose, 20% solution in pipet 24 Glucose test strips 1 Graduated Cylinder, plastic, 50 mL 2 Lactase pills 1 Pencil, marking 10 Pipet, Empty Long Stem 6 Plastic cup, 9 oz 1 Scale-Digital – iMPoRTANT !!! – If you do not perform the following two steps your scale will not work properly: 1) If present, on the side of the scale underneath the weighing platform, pull out the small plastic strip which is attached to a piece of cardboard that protects the scale during shipping, and 2) Remove the plastic strip between the battery & connectors, or if the batteries are shrink- wrapped, the shrink-wrap must be removed. 1 Test-tube-clamp-holder 1 Test-tube-cleaning-brush 1 Test Tube (3), 13 x 100 mm in Bubble Bag 1 Test-tube-rack -6x13-mm 1 Thermometer-in-cardboard-tube 1 Well-Plate -12 1 Gloves, disposable - pair 1 Rod, Stirring - glass www.LabPaq.com 127 ©Hands-On Labs, Inc. Experiment EnzymEs TEmpEraTurE pH spEcificiTy Enzymes 1 pH 11.5, Buffer- 2 mL in Pipet 1 pH 3.5, Buffer - 2 mL in Pipet 1 pH 5.0, Buffer - 2 mL in Pipet 1 pH 6.8, Buffer - 2 mL in Pipet Note: The packaging and/or materials in this LabPaq may differ slightly from ...
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Enzymes
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Enzymes - VIP
molecules. Mr Mark Gretgrix Wedderburn College
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Mode of Operation
Enzymes act to orient the reactant/s so that they behave exactly as required for the reaction to occur.
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a decomposition reaction:
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Sucrose
Glucose + fructose:
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