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Bioinformatic Harvester II Education and Training A cademia  S inica I nstitute of  B io m edical  S ciences Biomedical IT Core Ming-Fang Tsai [email_address]
Agenda 1. Overview 3. Introduction to Databases 4. Summary 2. Advance Search
Overview
Feature Overview ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
http://harvester.embl.de ,[object Object],[object Object],[object Object],[object Object]
Flow Diagram Text results Genome Gene position localization Domain : : Graphical results Sequence Saved as a file Human Mouse Rat Cross Link Input  page rank
General Query chromosome 4q32 human chromosome  4q32 107 筆 P42262 P42262 Text results Genome Graphical results Save as a  file Input
General Query chromosome 4q32 human chromosome  4q32 brain brain hypothetical Text results Genome Graphical results Save as a  file Input 善用  AND 及 NOT 功能 , 可有效縮小範圍 , 原 107  筆減為 20 筆資料
Exercise  ,[object Object],[object Object]
6q21-6q24, not hypothetical 可以使用自定 protein list 的功能 , 稍後將會介紹 Q:  如果想查  6q21,6q24, brain  的資料 , 要怎麼做呢 ?
如何將查詢結果另存新檔 ? ,[object Object],Text results Genome Graphical results Save as a  file Input Text results 跟 graphical results 都是相同方式存檔哦 ! Graphical results
Advance Search
User Defined Protein List ,[object Object],[object Object],2345 第一次需設定密碼 , 以後憑此密碼調出資料 Protein set ID 回功能頁 Protein 之間以空格或換行分開 , 都可以
Protein-Set Search 2345 注意 :  密碼最好設定複雜一點 ,  萬一有人輸入相同密碼 , 則一樣可以存取你的資料 .
Exercise  ,[object Object],[object Object],[object Object],[object Object]
如何查詢 6q21, 6q24, brain? ,[object Object],得到  272 筆資料
如何查詢 6q21, 6q24, brain? ,[object Object]
[object Object],如何查詢 6q21, 6q24, brain? 複製欄位 B 的 protein list
如何查詢 6q21, 6q24, brain? ,[object Object]
Exercise  ,[object Object],[object Object]
Sequence Search
Sequence Search QARENKDFVR http://www-db.embl.de/jss/servlet/de.embl.bk.htmlfind.HarvesterOutputMysql?m = seqSearch 此處可以是任意長度任意字 ,[object Object],[object Object]
Sequence Pattern Search ,[object Object],[object Object],[object Object],[object Object],[object Object]
Send sequence to STRING,BLAST,SMART, CDART  ,[object Object],[object Object]
Term Search by application ,[object Object],[object Object],[object Object],[object Object],[object Object]
Sequence Search by application ,[object Object],[object Object]
Template ,[object Object],keyword url  連結方法 sequence
Practice  ,[object Object],[object Object],http://www-db.embl.de/jss/servlet/de.embl.bk.htmlfind.HarvesterOutputMysql?m = seqSearch ,[object Object]
Introduction to Databases
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
http://genome-www5.stanford.edu/cgi-bin/source/sourceSearch
 
Source Batch Search  ,[object Object]
GFP-cDNA ,[object Object]
GFP-cDNA ,[object Object],[object Object],[object Object]
GFP-cDNA ,[object Object],輸入方式跟 Harvester 一樣
SMART ,[object Object],[object Object],[object Object],[object Object],[object Object]
SMART ,[object Object],[object Object],[object Object],[object Object],[object Object]
SMART ,[object Object]
SMART ,[object Object]
PSORT  ,[object Object],[object Object],[object Object]
SOSUI ,[object Object],[object Object],[object Object],[object Object],[object Object]
SOSUI Result http://sosui.proteome.bio.tuat.ac.jp/cgi-bin/adv_sosui.cgi
iHOP ,[object Object],Gene defining information interaction
GoPubmed ,[object Object],[object Object]
STRING ,[object Object]
Summary
Summary ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Thank you!

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