Scaling API-first – The story of a global engineering organization
NSA Feb 2010: DNA Methylation Patterns & Epigenetic Regulation in the Pacific Oyster
1. DNA Methylation Patterns & Epigenetic Regulation in the Pacific Oyster Mackenzie Gavery & Steven Roberts University of Washington School of Aquatic and Fishery Sciences
11. DNA methylation Effects of disruptions: tumor promotion alteration of development inhibition of reproduction Compounds that impact normal epigenetic functions: Endocrine disruptors: estrogen, BPA, pesticides
12. Characterization of DNA methylation in Pacific oysters: describe distribution of methylation elucidate functional significance
15. Results: gene-targeted approach Methylation Sensitive PCR 5 stress related genes were examined Identified CpG methylation in heat shock protein 70 Bisulfite sequencing 136 bp fragment: 1 of 7 CpG methylated (homology to neuromedin-u receptor) 93 bp fragment: 1 of 2 CpG methylated (homology to bromodomain adjacent to zinc finger domain)
17. Results Methylation Sensitive PCR Bisulfite sequencing In silicoanalysis predicted methylation status of 12,000 C. gigas sequences from GigasBase sequences were grouped by Gene Ontology term an average predicted methylation status was determined
22. Implications: Selective Breeding Selective breeding can contribute to improved & predictable performance in oysters Understanding geneticand epigenetic influences will increase predictability
23. Implications: Hybrid Vigor Heterosis (hybrid vigor) mechanism not fully understood epigenetic mechanisms have been proposed better understanding will allow for greater control in predicting and manipulating gene expression in oysters X =
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25. Future Directions Method evaluation/development: challenges associated with non-model species new approaches: Whole genome bisulfite sequencing (BS-seq) Methylated DNA immunoprecipitation (MeDIP) MeDIP-seq MeDIP-chip
26. Summary Characterization of DNA methylation in Pacific oyster suggests a role in gene regulation, specifically genes with inducible expression DNA methylation could be an important mechanism contributing to phenotypic variation in oysters Important evaluate & develop methods and tools to evaluate epigenetic mechanisms in bivalves
27. Acknowledgements UW, SAFS Dr. Steven Roberts Samuel White Lisa Crosson Emma Timmins-Schiffman Taylor Shellfish Farms Joth Davis NSA-PCS NOAA Aquaculture Program