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Lecture # 1: Molecular and Cellular Biology Overview  http:// www.youtube.com/watch?v =FZtRn1fst-g
WELCOME!
instructor Mark E Minie PhD [email_address]
Class mechanics…
The wiki… http:// uweobasicbiomolcelli.wikispaces.com /
[object Object],[object Object],[object Object],[object Object],[object Object]
A word about PMIDs and  UW restricted resources…
Tonight’s timeline: 5:30 PM-6:30 PM: Shannon Kuyper Orientation 6:30 PM-6:45 PM: Break 6:45 PM-7:45 PM: Lecture # 1 7:45 PM-8:00 PM: General discussion and Q&A
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
What is life?: Dictionary.com  Unabridged (v 1.1)  -  Cite This Source  -  Share This   life            laɪf/  Pronunciation Key  -  Show Spelled  Pronunciation [lahyf]  Pronunciation Key  -  Show IPA Pronunciation   noun, plural lives          laɪvz/  Pronunciation Key  -  Show Spelled  Pronunciation [lahyvz]  Pronunciation Key  -  Show IPA Pronunciation ,  adjective  – noun  1.the condition that distinguishes organisms from inorganic objects and dead organisms, being manifested by growth through metabolism, reproduction, and the power of adaptation to environment through changes originating internally.  2.the sum of the distinguishing phenomena of organisms, esp. metabolism, growth, reproduction, and adaptation to environment.
[object Object]
[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],The expanding universe of prion diseases. PMID: 16609731
[object Object],[object Object],Hayflick, his limit, and cellular ageing.  PMID: 11413492
[object Object],http:// en.wikipedia.org/wiki/Spore_(game )
[object Object],[object Object],A 1.7-kilobase single-stranded DNA that folds into a nanoscale octahedron.  PMID: 14961116  Functional DNA nanotechnology: emerging applications  of DNAzymes and aptamers.  PMID: 17056247
Are aliens among us?  PMID: 18237098
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object]
There are two different types of cells on Earth*… *Really three, but we won’t be talking about the Archaea today :)  Prokaryote Eukaryote plant animal
And they are simultaneously the same  but very different in structure, function,  composition, behavior, etc...
Prokaryotic cell structure
Eukaryotic animal cell structure
Eukaryotic plant cell structure
Size-eukaryotes are generally bigger than prokaryotes
prokaryotes & eukaryotes have different  genetic structures  as well…
[object Object],[object Object],[object Object]
THE TREE OF LIFE Fun with genealogy.  PMID: 9398070
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Model systems
[object Object],[object Object]
Molecular motors http:// www.youtube.com/watch?v =InIha7bCTjM&feature=related
[object Object],[object Object],[object Object]
The central dogma of molecular biology
The sequences of these biomolecules are digital in nature… and thus lend themselves to digital analysis with computers… DNA Protein … arrsrarklqrmkql … … tggagatgacgtca … … 0111000101011111 … … 010110101110000 … RNA … uggagaugacguca … … 0111000101011111 …
[object Object],[object Object],[object Object],[object Object]
http:// www.ncbi.nlm.nih.gov/sites/gquery A key bioinformatics resource—ENTREZ  The National Center for Biotechnology Information (NCBI)
[object Object],[object Object]
deoxyribonucleotide sequences self-organize  into a double helix, amino acid sequences self-organize into various peptide structural motifs…
and it is this informational and self-organizing chemical  nature that forms the basis for all structural and behavioral activities of cells…
and is the basis for our current concepts of the origin of cellular life on Earth…
“ Classic” Miller-Urey Experiment
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Metabolome The sum total of the chemical  reactions and pathways in a given organism
[object Object],[object Object]
A “simple” metabolic pathway…GLYCOLYSIS http:// www.biocarta.com/pathfiles/h_glycolysisPathway.asp
http:// www.expasy.ch /tools/pathways/ Metabolic pathways and cellular processes THE BIG PICTURE
These cellular processes and metabolic pathways are  mediated by proteins, carbohydrates, lipids, DNA, RNA and other molecular entities and function in  “ real time”—they are only indirectly regulated and  controlled by the cellular gene structure…
http:// www.biocyc.org / Computer generated models of metabolic pathways…
[object Object],[object Object],[object Object]
Vast amounts of data at every level, with the blurring barriers between biology and information technology  DNA Sequence Data RNA Expression Data Protein Structure Data
http:// www.youtube.com/watch?v =5ukPD4G5eSw  The inner complexity of a cell (eukaryote)…
Open discussion and Q&A

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Lecture 1 Molecular And Cellular Biology Overview

  • 1. Lecture # 1: Molecular and Cellular Biology Overview http:// www.youtube.com/watch?v =FZtRn1fst-g
  • 3. instructor Mark E Minie PhD [email_address]
  • 5. The wiki… http:// uweobasicbiomolcelli.wikispaces.com /
  • 6.
  • 7. A word about PMIDs and UW restricted resources…
  • 8. Tonight’s timeline: 5:30 PM-6:30 PM: Shannon Kuyper Orientation 6:30 PM-6:45 PM: Break 6:45 PM-7:45 PM: Lecture # 1 7:45 PM-8:00 PM: General discussion and Q&A
  • 9.
  • 10. What is life?: Dictionary.com Unabridged (v 1.1) - Cite This Source - Share This life       laɪf/ Pronunciation Key - Show Spelled Pronunciation [lahyf] Pronunciation Key - Show IPA Pronunciation noun, plural lives       laɪvz/ Pronunciation Key - Show Spelled Pronunciation [lahyvz] Pronunciation Key - Show IPA Pronunciation , adjective – noun 1.the condition that distinguishes organisms from inorganic objects and dead organisms, being manifested by growth through metabolism, reproduction, and the power of adaptation to environment through changes originating internally. 2.the sum of the distinguishing phenomena of organisms, esp. metabolism, growth, reproduction, and adaptation to environment.
  • 11.
  • 12.
  • 13.
  • 14.
  • 15.
  • 16.
  • 17. Are aliens among us? PMID: 18237098
  • 18.
  • 19.
  • 20. There are two different types of cells on Earth*… *Really three, but we won’t be talking about the Archaea today :) Prokaryote Eukaryote plant animal
  • 21. And they are simultaneously the same but very different in structure, function, composition, behavior, etc...
  • 25. Size-eukaryotes are generally bigger than prokaryotes
  • 26. prokaryotes & eukaryotes have different genetic structures as well…
  • 27.
  • 28. THE TREE OF LIFE Fun with genealogy. PMID: 9398070
  • 29.
  • 30.
  • 31. Molecular motors http:// www.youtube.com/watch?v =InIha7bCTjM&feature=related
  • 32.
  • 33. The central dogma of molecular biology
  • 34. The sequences of these biomolecules are digital in nature… and thus lend themselves to digital analysis with computers… DNA Protein … arrsrarklqrmkql … … tggagatgacgtca … … 0111000101011111 … … 010110101110000 … RNA … uggagaugacguca … … 0111000101011111 …
  • 35.
  • 36. http:// www.ncbi.nlm.nih.gov/sites/gquery A key bioinformatics resource—ENTREZ The National Center for Biotechnology Information (NCBI)
  • 37.
  • 38. deoxyribonucleotide sequences self-organize into a double helix, amino acid sequences self-organize into various peptide structural motifs…
  • 39. and it is this informational and self-organizing chemical nature that forms the basis for all structural and behavioral activities of cells…
  • 40. and is the basis for our current concepts of the origin of cellular life on Earth…
  • 42.
  • 43. Metabolome The sum total of the chemical reactions and pathways in a given organism
  • 44.
  • 45. A “simple” metabolic pathway…GLYCOLYSIS http:// www.biocarta.com/pathfiles/h_glycolysisPathway.asp
  • 46. http:// www.expasy.ch /tools/pathways/ Metabolic pathways and cellular processes THE BIG PICTURE
  • 47. These cellular processes and metabolic pathways are mediated by proteins, carbohydrates, lipids, DNA, RNA and other molecular entities and function in “ real time”—they are only indirectly regulated and controlled by the cellular gene structure…
  • 48. http:// www.biocyc.org / Computer generated models of metabolic pathways…
  • 49.
  • 50. Vast amounts of data at every level, with the blurring barriers between biology and information technology DNA Sequence Data RNA Expression Data Protein Structure Data
  • 51. http:// www.youtube.com/watch?v =5ukPD4G5eSw The inner complexity of a cell (eukaryote)…