Week 5 Assignment B View the assignment video on the week 5 page FIRST. This is one of the more difficult assignments you will do all semester. Work your way through one step at a time, using the examples to help you. If you need help, ASK! The crime lab collected evidence from the scene of the crime – specifically, hair and blood samples. Now it is time for you to analyze the data and figure out “who done it”. You will do this by creating a DNA fingerprint with the samples that were collected. As you learned in the mini-lecture, a DNA fingerprint is collected using the following methods · Cut the DNA into pieces using restriction enzymes · Copy the DNA many times to get more of it to work with · “Run” the DNA through a gel to separate the different pieces (gel electrophoresis), which will separate the pieces of cut DNA by size You are going to do this for the various samples you collected from the crime scene, and use that data to solve the crime. Step 1.Cut the DNA into pieces using restriction enzymes. You use the restriction enzyme HaeIII to cut the DNA into pieces. HaeIII cuts the following way: AAGGCCTAG gets cut into AAGG CCTAG TTCCGGATC TTCC GGATC So, every time the enzyme encounters the sequence “GGCC”, the DNA strand it cut between the G’s and C’s. The DNA samples we are working with contain 2 short tandem repeat (STR) segments. The first repeat is CCACGT, and the second is CGC. Cut the DNA with the restriction enzyme, HaeIII. The first DNA sample (victim) is cut for you. Determine how many base pairs (bp) long each piece of DNA is once it has been cut. (a) Victim: CCGAGAGGCCCCACGTCCACGTGGCCCGCCGCCGCCGCCGCCGCCGCGGCCA GGCTCTCCGGGGTGCAGGTGCACCGGGCGGCGGCGGCGGCGGCGGCGCCGGT Answer: Site 1: (16bp long) Site 2: (25bp long) CCGAGAGG CCCCACGTCCACGTGG CCCGCCGCCGCCGCCGCCGCCGCGG CCA GGCTCTCC GGGGTGCAGGTGCACC GGGCGGCGGCGGCGGCGGCGGCGCC GGT The enzyme cut each time the target sequence appeared in our DNA sample. We counted the length (in base pairs) of the DNA pieces between the cuts. Cut the remaining DNA samples yourself, and determine how many base pairs each is. (b) Suspect #1: CCAGGCCCCACGTCCACGTCCACGTGGCCCGCCGCCGCGGCCTAAAGAGGCTA GGTCCGGGGTGCAGGTGCAGGTGCACCGGGCGGCGGCGCCGGATTTCTCCGAT Answer: Site 1 Site #2 c) Suspect #2 CCAGGCCCCACGTGGCCCGCCGCCGCCGCCGCCGCCGCCGCCGCGGCCTAAA GGTCCGGGGTGCACCGGGCGGCGGCGGCGGCGGCGGCGGCGGCGCCGGATTT Answer: Site 1 Site #2 d) Evidence (hair sample) CCAGGCCCCACGTGGCCCGCCGCCGCCGCCGCCGCCGCCGCCGCGGCCTAAA GGTCCGGGGTGCACCGGGCGGCGGCGGCGGCGGCGGCGGCGGCGCCGGATTT Answer: Site 1 Site #2 e) Evidence (blood sample) CCGAGAGGCCCCACGTCCACGTGGCCCGCCGCCGCCGCCGCCGCCGCGGCCA GGCTCTCCGGGGTGCAGGTGCACCGGGCGGCGGCGGCGGCGGCGGCGCCGGT Answer: Site 1 Site #2 Step 2. You use a machine to make many copies of your DNA pieces. (assignment continues below) Step 3.Gel Electrophoresis. You place you cut DNA sample.

1. Week 5 Assignment B View the assignment video on the week
5 page FIRST. This is one of the more difficult assignments you
will do all semester. Work your way through one step at a time,
using the examples to help you. If you need help, ASK!
The crime lab collected evidence from the scene of the crime –
specifically, hair and blood samples. Now it is time for you to
analyze the data and figure out “who done it”. You will do this
by creating a DNA fingerprint with the samples that were
collected.
As you learned in the mini-lecture, a DNA fingerprint is
collected using the following methods
· Cut the DNA into pieces using restriction enzymes
· Copy the DNA many times to get more of it to work with
· “Run” the DNA through a gel to separate the different pieces
(gel electrophoresis), which will separate the pieces of cut DNA
by size
You are going to do this for the various samples you collected
from the crime scene, and use that data to solve the crime.
Step 1.Cut the DNA into pieces using restriction enzymes. You
use the restriction enzyme HaeIII to cut the DNA into pieces.
HaeIII cuts the following way:
AAGGCCTAG gets cut into AAGG CCTAG
TTCCGGATC TTCC
GGATC
So, every time the enzyme encounters the sequence “GGCC”,
the DNA strand it cut between the G’s and C’s.

2. The DNA samples we are working with contain 2 short tandem
repeat (STR) segments. The first repeat is CCACGT, and the
second is CGC. Cut the DNA with the restriction enzyme,
HaeIII. The first DNA sample (victim) is cut for you. Determine
how many base pairs (bp) long each piece of DNA is once it has
been cut.
(a) Victim:
CCGAGAGGCCCCACGTCCACGTGGCCCGCCGCCGCCGCC
GCCGCCGCGGCCA
GGCTCTCCGGGGTGCAGGTGCACCGGGCGGCGGCGGCGG
CGGCGGCGCCGGT
Answer:
Site 1: (16bp long) Site 2: (25bp long)
CCGAGAGG CCCCACGTCCACGTGG
CCCGCCGCCGCCGCCGCCGCCGCGG CCA
GGCTCTCC GGGGTGCAGGTGCACC
GGGCGGCGGCGGCGGCGGCGGCGCC GGT
The enzyme cut each time the target sequence appeared in our
DNA sample. We counted the length (in base pairs) of the DNA
pieces between the cuts. Cut the remaining DNA samples
yourself, and determine how many base pairs each is.
(b) Suspect #1:
CCAGGCCCCACGTCCACGTCCACGTGGCCCGCCGCCGCGG
CCTAAAGAGGCTA
GGTCCGGGGTGCAGGTGCAGGTGCACCGGGCGGCGGCGC
CGGATTTCTCCGAT
Answer: Site 1 Site #2
c) Suspect #2

3. CCAGGCCCCACGTGGCCCGCCGCCGCCGCCGCCGCCGCC
GCCGCGGCCTAAA
GGTCCGGGGTGCACCGGGCGGCGGCGGCGGCGGCGGCGG
CGGCGCCGGATTT
Answer: Site 1 Site #2
d) Evidence (hair sample)
CCAGGCCCCACGTGGCCCGCCGCCGCCGCCGCCGCCGCC
GCCGCGGCCTAAA
GGTCCGGGGTGCACCGGGCGGCGGCGGCGGCGGCGGCGG
CGGCGCCGGATTT
Answer: Site 1 Site #2
e) Evidence (blood sample)
CCGAGAGGCCCCACGTCCACGTGGCCCGCCGCCGCCGCC
GCCGCCGCGGCCA
GGCTCTCCGGGGTGCAGGTGCACCGGGCGGCGGCGGCGG
CGGCGGCGCCGGT
Answer: Site 1 Site #2
Step 2. You use a machine to make many copies of your DNA
pieces.
(assignment continues below)
Step 3.Gel Electrophoresis. You place you cut DNA samples
into a gel electrophoresis machine. You have already
determined how big each of your DNA pieces is (above). Use

4. that information to draw what the gel would look from these
samples. Each sample (victim, hair, etc) will have 2 bands on
the gel – once for each of the cut pieces of DNA. Draw those on
the gel below. The first (victim) is done for you.
Tech Tip: To draw on the gel, click on one of existing purple
lines for the victim, copy, then paste. Move the line to the
appropriate space on the gel. You may not be able to get the
line exactly where you want it – but close counts in this
assignment!
(
Victim
) (
Blood
) (
Hair
) (
S #2
) (
S #1
) Gel
(
50
bp
)
(
40
bp
)

5. (
15
bp
) (
5
bp
) (
10
bp
) (
20
bp
) (
30
bp
)(assignment continues below)
Step 4.Analysis. Based on the DNA evidence above, answer the
following questions.
a) What conclusions can you make about the DNA collected
from the hair sample left at the scene of the crime? How do you
know?
b) What conclusions can you make about the DNA collected
from the blood sample left at the scene of the crime? How do
you know?
c) Who do you think committed the crime? What evidence is
there to support this?