1. Genetic Engineering Biology - Edgar

2. Fig. 20-3-1 Restriction site DNA Sticky end Restriction enzyme cuts sugar-phosphate backbones. 5  3  3  5  1

3. Fig. 20-3-2 Restriction site DNA Sticky end Restriction enzyme cuts sugar-phosphate backbones. 5  3  3  5  1 DNA fragment added from another molecule cut by same enzyme. Base pairing occurs. 2 One possible combination

4. Fig. 20-3-3 Restriction site DNA Sticky end Restriction enzyme cuts sugar-phosphate backbones. 5  3  3  5  1 One possible combination Recombinant DNA molecule DNA ligase seals strands. 3 DNA fragment added from another molecule cut by same enzyme. Base pairing occurs. 2

5. Fig. 20-9a Mixture of DNA mol- ecules of different sizes Power source Longer molecules Shorter molecules Gel Anode Cathode TECHNIQUE 1 2 Power source – + + –

9. Fig. 20-10 Normal allele Sickle-cell allele Large fragment (b) Electrophoresis of restriction fragments from normal and sickle-cell alleles 201 bp 175 bp 376 bp (a) Dde I restriction sites in normal and sickle-cell alleles of  -globin gene Normal  -globin allele Sickle-cell mutant  -globin allele Dde I Large fragment Large fragment 376 bp 201 bp 175 bp Dde I Dde I Dde I Dde I Dde I Dde I

11. Genetic Engineering & Cloning

12. Herbert Boyer and Stanley Cohen

16. Isolate DNA

17. Restriction Enzymes

19. Cut DNA

35. Recombinant DNA

42. Polymerase Chain Reaction

49. GMO Lab

55. Fig. 12-8a-1 Agrobacterium tumefaciens DNA containing gene for desired trait Ti plasmid Insertion of gene into plasmid Recombinant Ti plasmid 1 Restriction site

56. Fig. 12-8a-2 Agrobacterium tumefaciens DNA containing gene for desired trait Ti plasmid Insertion of gene into plasmid Recombinant Ti plasmid 1 Restriction site Plant cell Introduction into plant cells 2 DNA carrying new gene

57. Fig. 12-8a-3 Agrobacterium tumefaciens DNA containing gene for desired trait Ti plasmid Insertion of gene into plasmid Recombinant Ti plasmid 1 Restriction site Plant cell Introduction into plant cells 2 DNA carrying new gene Regeneration of plant 3 Plant with new trait

62. 11,497 4700 2838 1700 1159 805 Pst I PstI 923 SspI 2586 HpaI 849 1257