Postexposure Administration of a Yersinia pestis Live Vaccine for Potentiation of Second-Line Antibiotic Treatment Against Pneumonic Plague

1. MATEO MEJIA POSADA
THIRD SEMESTER
Medicine
2019-II

2. INTRODUCTION
YERSINIA
PESTIS
3-4 Days
• Aminoglycosides
• Fluoroquinolones
• Tetracyclines
• Chloramphenicol
EFFECTIVENESS?

3. INTRODUCTION
SECOND LINE ANTIBIOTIC
TREATMENT
Vaccines

4. GENERAL OBJECTIVE
EVALUATION OF POSTEXPOSURE
ADMINISTRATION OF YERSINIA PESTIS VACCNINE
FOR POTENTIATION OF SECOND-LINE ANTIBIOTIC
TREATMENT AGAINST PNEUMONIC PLAGUE.
Postexposure Administration of a Yersinia pestis Vaccine for
Potentiation of Second-Line Antibiotic Treatment Against
Pneumonic Plague

5. ANIMALS
BASIS
•
FOR WHAT?
•
METHODS

6. Western blot
Basis
 Evaluate the reaction between
an antigen and an antibody,
and electrophoresys is done to
separate proteins and those
proteins are identified with a
specific antibody.
For what?
 Quantify haptoglibin and
hemopexin in a model of
pneumonic plague in mice
differently treated by using
anti-Hp (Abcam ab131236) and
Anti-Hx (Abcam ab90947)
ANTIBODIES.
METHODS

7. Antibiotic treatment
Basis
 Antibiotics are powerful
medicines that fight certain
infections and can save lives
when used properly. They
either stop bacteria from
reproducing or destroy them.
For what?
 Evaluate if there could be any
synergistic protective effect
between second line treatment
(ceftriaxone), and the EV76
live vaccine.
METHODS

8. RESULTS

9. RESULTS

10. RESULTS

11. DISCUSSION
AUTHOR WHAT HE SAID RIGHT OR WRONG?
Zauberman A, Vagima Y, Tidhar A,
et al.
In reporting a previous study, we
suggested a new concept by which live
attenuated vaccines have the
potential to induce rapid iron
nutritional immunity beneficial for
postexposure therapy by enabling the
extension of the time window
necessary for the development of an
acquired specific immune response.
Zauberman A, Vagima Y, Tidhar A,
et al.
Administration of the Y. pestis
EV76 attenuated vaccine strain,
concomitantly with the challenge,
protected against bubonic plague
and was accompanied by up-
regulation of the host iron-
chelating protein, Hx.

12. DISCUSSION
AUTHOR WHAT HE SAID RIGHT OR WRONG?
Eaton JW, Brandt P,
Mahoney JR, Lee JT Jr.
Hp and Hx can accelerate
the clearance of free
hemoglobin and heme,
limiting the availability of
the iron necessary for
bacterial growth.

13. CONCLUSIONS
“The- current findings
study therefore provide a
proof of concept whereby
combinational therapy
could serve as an
alternative in outbreaks of
pneumonic plague by
antibiotic resistant
strains.”
Even though
administration of EV76
vaccine could increase
the life expectancy of the
affected by the
pneumonic plague, the
better results are
achieved in combination
with the ceftriaxone
treatment, and the
experimental analysis
should be based on their
interaction together.

14. Map