LifeTein® is the only peptide manufacturer to offer the PeptideSynTM platform of peptide synthesis services. PeptideSynTM technology is optimized to provide a continuous flow of synthesized peptides, reducing costs while maintaining quality. PeptideSynTM has been proven to enhance the efficiency of synthesis.
3. Synthetic Therapeutic Peptides
More than 60 synthetic therapeutic peptides under 50 amino acids in
size have reached the pharmaceutical market as APIs or generics. 3
4. Chemical Optimization
Drug Discovery Today Volume 15, Numbers 1/2 January 2010
Strategies for Lead Peptide Optimization 4
5. Technology Trends
Microwave Synthesis
Using Nitroalkanes as Acyl Anion
Equivalents
www.nature.com/doifinder/10.1038/nature09125
Green Chemistry for Synthesis
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6. LifeTein Technology Portfolio
PeptideSynTM Platform
Provides high-yield
peptide synthesis
Combines SPPS and
LPPS for the highest
synthesis success rate
possible
Ensures shorter
turnaround
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7. LifeTein Technology Portfolio
PeptideSynTM Ligation
Synthesizes long
peptides (up to 120
amino acids)
Ensures high yield and
short turnaround
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9. LifeTein Services
Custom Peptide Synthesis Service
Custom Peptide Library Synthesis Service
Custom Polyclonal Antibody Service
Custom Monoclonal Antibody Service
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10. LifeTein Services
Advantages of PeptideSynTM Technology
Increases yields and reduces costs
Shortens production cycle
Combines the advantages of both solid and solution phase methods
Can scale up projects rapidly to over 100 kg
Avoids solubility problems for long segments in solution phase
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11. Peptide Synthesis Service
Peptide Length 5-120 amino acids
Peptide Scale From mg to kg
Peptide Purity Crude, desalted, >75%, >85%, >90%, >95%, 98%, and >99%
Peptide
2-5 phosphorylation sites, methylation, acetylation, amidation, MAPs(2, 4 or 8 chains), more…
Modifications
Peptide Labels FITC, Biotin, 5-FAM, Rhodamine, Dansyl, TAMRA, more…
Carrier Proteins KLH, BSA, OVA
Cyclic Peptides Amide cyclic peptide (head to tail, side chain), 1, 2, or 3 disulfide bond bridges
Analysis HPLC, MS, Amino Acid Analysis (AAA), solubility testing, microbial analysis, more…
Peptide Libraries Peptide sequence length of 5-20 amino acids, 48, 96 peptides
Others Free aliquots, spacers and linkers (Ahx, mini-PEG), more…
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13. Case Studies
Cell Penetrating Peptides
Name Origin Sequence
Tat family
Tat (48-60) HIV-1 protein GRKKRRQRRRPPQQ
Oligoarginine Tat derivative Rn
Penetralia family
p-Antp Antermapedia homeodomain RQIKIWFQNRRMKWKK
plsl Igl-1 homeodomain RVIRVWFQNKRCKDKK
Chimeric CPPs
GWTLNSAGYLLGKINLKALAA
Transportan Galanin-mastoparan
LAKKIL
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14. Case Studies
Cell Penetrating Peptides
Attached to a CPP, therapeutic cargo is delivered to an intracellular target,
thus overcoming the entry restrictions set by the plasma membrane. 14
15. Case Studies
N-Terminal Acetylation and C-Terminal Amidation
Both modifications are free of charge
Chemically synthesized peptides have free amino and carboxyl termini.
N-terminal acetylation and C-terminal amidation reduce the overall charge of the peptide. This can 15
increase the stability of the peptide and its resemblance to the native protein.
17. Peptide Antigen Design
Sequence Length 8-20 amino acids
• >75% for antibody generation,
Peptide Purity
• >90% for phosphorylated peptide antibody (recommended)
• Keep the hydrophobic amino acid content below 50%
Peptide Solubility
• At least one charged residue for every five amino acids
• Cys and Met can undergo rapid oxidation
Difficult Amino Acids • Avoid multiple Ser or Pro
• Avoid multiple or adjacent β-sheet forming amino acids: Glu, Iso, Leu, Phe, Thr, Tyr, Val
Carrier Proteins KLH, BSA, OVA
Multiple Antigenic Peptides (MAPs)
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20. Contact Information
LifeTein LLC
150 Maple Ave
South Plainfield, NJ 07080
USA
info@lifetein.com
1-732-444-3282
1-888-791-1618 (Toll Free)
1-888-791-1618 (Fax)
Let us be a partner to your growth!
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