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Molecular Biology 1-2

         put together by: Linda Fahlberg-Stojanovska

    Disclaimer: I put these together for my kid for his smartphone.
 However, I found most images had very small type and increased the
font size. I am posting it because another teacher might find this useful.
                       The sources are given.
     If I have used anything illegally, write me and I will take it off.

                                                                           1
Contents
• Cell Structure - Eukaryotes
• Cell Parts - Eukaryotes
• Bacteria
  – Gram Positive / Negative
• Viruses
Eukaryote Cell




                                                     3
            http://www.biologycorner.com/bio1/cell.html
Eukaryote




                                             4
http://www.enchantedlearning.com/subjects/animals/cell/
Eukaryote




                                             5
http://www.enchantedlearning.com/subjects/plants/cell/
Endoplasmic Reticulum (ER)




                                                6
                       http://endoplasmicreticulum.net/
Golgi apparatus (GA)
• Like a post office - it packages and labels items
  and then sends to different parts of the cell.
• It primarily modifies proteins from the rough ER
• transport of lipids
• creation of lysosomes
• creation of polysacharides
• Modify, sort, and store macromolecules
  for cell secretion (exocytosis)
     In glandular (secretion) cells > 100 GA.

                                                      7
Golgi apparatus (GA)




                                                 8
http://www.biology4kids.com/files/cell_er.html
Golgi apparatus (GA)




                                                                                                            9
http://creationrevolution.com/2010/11/golgi-apparatus-steel-industry-of-the-simple-cell-%E2%80%93-part-6/
Mitochondria- animals                   Chloroplast - plant

Mitochondria = organelles that produce energy ATP
 • Both plants and animals have mitochondria, but
   plants produce ATP in their chloroplasts.
 • signaling,
 •   cellular differentiation,
 •   control of cell cycle; cell growth and death,
 •   Mitochondria have a double membrane
      • Outer is relatively smooth; Inner is very convoluted
      • The membrane proteins are part of cellular
        respiration and the synthesis of compounds, they are
        energy dense with ATP adenosintriphosphate).

                                                               10
Mitochondria- animals                            Chloroplast - plant




                                                                       11
        http://en.wikipedia.org/wiki/Mitochondrion
Chloroplast - plant           Mitrochondria – animal

Chloroplasts are organelles
   • found in plant cells
   • conduct photosynthesis , that is the chemical
     process that converts carbon dioxide into
     organic compounds, especially sugars, using
     the energy from sunlight.
   • capture light energy to conserve free energy
     in the form of ATP.
   • are green because they contain the
     chlorophyll pigment.
   • are members of a class of organelles known
     as plastids.
                                                       12
Chloroplast - plant                             Mitrochondria – animal




                                                                         13
      http://www.tutorvista.com/biology/chloroplasts
Lysosome - animal                             Vacuoles – plant
• Lysosomes are organelles
• They contain acid hydrolase enzymes.
      hydrolase is a digestive enzyme that catalyzes the
      hydrolysis of a chemical bond (breaks down polymers).
• Lysosomes break down waste
  materials and cellular debris.
  •    Lysosomes also digest food particles,
       and engulfed viruses or bacteria.
  •    The membrane around a lysosome
       allows the digestive enzymes to work at
       the 4.5 pH they require.


                                                                                   14
                                          http://www.biology4kids.com/files/cell_lysosome.html
Lysosome - animal




                                                                    Phagocytosis
                                                         Lysosome digesting food



                                                                                   15
  http://apbio-werle.wikispaces.com/Cell+and+Cell+Transport+%28Ch+6-7%29
Vacuoles - plant                                     Lysosomes – animals

• Vacuoles are large, liquid-filled organelles found
  only in plant cells, occupying up to 90% of a cell's
  volume and have a single membrane.

                           Vacuole
 •   Their main function is as a
     space-filler in the cell but
     they also perform digestive
     functions similar to
     lysosomes.



                                                                                16
            http://www.biologycorner.com/APbiology/cellular/notes_cells2.html
Plasma membrane = Cell membrane
• The cell membrane or plasma membrane is a biological
  membrane that separates the interior of all cells from the
  outside environment.
• Cell membrane is semi-permeable (selectively
  permeable) to ions and organic molecules and controls the
  movement of substances in and out of cells.
• It consists of the lipid bilayer with embedded proteins.
• Cell membranes serve as the attachment surface for the
  cell wall, glycocalyx, and intracellular cytoskeleton.



                                                               17
Plasma membrane = Cell membrane




                                  18
Cytoskeleton
                                                  • The cytoskeleton
                                                    (CSK) is a cellular
                                                    "skeleton" made out of
                                                    protein. It is within a
                                                    cell's cytoplasm.
                                                  • Eukaryotic cells contain
                                                     3 main kinds of
                                                     cytoskeletal filaments:
                                                    – microfilaments,
                                                    – intermediate filaments
                                                    – microtubules.


                                                                                         19
http://www.sparknotes.com/biology/cellstructure/intracellularcomponents/section1.rhtml
20
Bacteria Cell                                                   Prokaryote




                                                                             21
        http://en.wikipedia.org/wiki/Bacterial_cell_structure
Bacteria
• Bacteria are divided into two major groups:
         Gram-negative and Gram-positive

• Gram-negative bacteria
   – Inner membrane
   – Thin layer of peptidoglycan in periplasmic space
   – Outer membrane = lipopolysaccharide + protein




                                                        22
Bacteria
• Bacteria are divided into two major groups:
         Gram-negative and Gram-positive
• Bacteria come in 4 basic shapes:
  – Baccilli (rod )
  – Cocci (spherical)
  – Spirilli (spiral)
  – Vibrios (comma)


                                                23
Bacteria - Shapes




                                                   24
 http://www.biotopics.co.uk/microbes/bacter.html
Bacteria – Gram Staining
• Gram-negative bacteria
  – Inner membrane
  – Thin layer of peptidoglycan in periplasmic space
  – Outer membrane = lipopolysaccharide + protein

• Gram-positive bacteria
  – Inner membrane
  – Thick layer of peptidoglycan called 'wall' (causes staining)
  – No outer membrane (usually)


                                                                   25
Bacteria – Gram Staining




                                                                                                 26
http://www.biologie.uni-hamburg.de/b-online/library/micro229/terry/images/micro/gramposneg.gif
Bacteria – Gram Staining
• Steps in Gram Staining
  – heat fix a smear of bacterial culture
  – apply a primary stain of crystal violet
  – add Gram’s iodine solution to bind crystal violet in the cell
  – rapid decolorization with alcohol or acetone
    Gram-positive cells are stained deep purple.
  – counterstain with safranin
    Gram-negative cells are stained red.



                                                                    27
Bacteria – Gram Staining




                                                  28
    http://pathmicro.med.sc.edu/fox/culture.htm
Bacteria – Gram Staining
• Reagents of Gram Staining
   – Crystal violet (primary stain)
   – Gram's Iodine solution
     (mordant that fixes crystal violet to cell wall)
   – Decolorizer (e.g. ethanol)
   – Safranin (secondary stain)
   – Water (preferably in a squirt bottle)



                                                        29
Bacteria – Gram Staining
• How Gram Staining Works
  – 2. Gram's iodine solution (iodine and potassium iodide) is
    added to form a complex between the crystal violet and iodine.
    This complex is a larger molecule than the original crystal violet
    stain and iodine and is insoluble in water.
  – 3. decolorizer dehydrates the peptidoglycan layer, shrinking
    and tightening it. The large crystal violet-iodine complex is
    trapped in the thick peptidoglycan layer of Gram positive
    bacteria and stained.
  – Conversely, the thin peptidoglycan layer of Gram negative cells
    cannot hold the crystal violet-iodine complex and the color
    (stain) is washed out and lost.

                                                                         30
Virus
  Virus particles (virions)
  •genetic material (DNA or RNA segment)
  •capsid (protein coat that protects these genes)
  •envelope (of lipids) (some virions)


                                                     Flu virus




http://www.abc.net.au/health/library/stories/2004/07/08/1831345.htm
                                                                      31
 http://www.synapses.co.uk/science/fluvirus.html
Virus - Bacteriophage
• The Capsid – Head. It is
  protein coat around nucleic
  acid. Capsid is divided into
  subunits called capsomeres.
• The Body - Rod shaped
  structure has retractible sheath
  around a central hollow core.
• The Tails – At end of core is
  spiked plate with 6 tail fibers
  which help anchor the virus to its
  host.

                                                               32
                http://www.armageddononline.org/viruses.html

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Molecular Biology 1-2

  • 1. Molecular Biology 1-2 put together by: Linda Fahlberg-Stojanovska Disclaimer: I put these together for my kid for his smartphone. However, I found most images had very small type and increased the font size. I am posting it because another teacher might find this useful. The sources are given. If I have used anything illegally, write me and I will take it off. 1
  • 2. Contents • Cell Structure - Eukaryotes • Cell Parts - Eukaryotes • Bacteria – Gram Positive / Negative • Viruses
  • 3. Eukaryote Cell 3 http://www.biologycorner.com/bio1/cell.html
  • 4. Eukaryote 4 http://www.enchantedlearning.com/subjects/animals/cell/
  • 5. Eukaryote 5 http://www.enchantedlearning.com/subjects/plants/cell/
  • 6. Endoplasmic Reticulum (ER) 6 http://endoplasmicreticulum.net/
  • 7. Golgi apparatus (GA) • Like a post office - it packages and labels items and then sends to different parts of the cell. • It primarily modifies proteins from the rough ER • transport of lipids • creation of lysosomes • creation of polysacharides • Modify, sort, and store macromolecules for cell secretion (exocytosis) In glandular (secretion) cells > 100 GA. 7
  • 8. Golgi apparatus (GA) 8 http://www.biology4kids.com/files/cell_er.html
  • 9. Golgi apparatus (GA) 9 http://creationrevolution.com/2010/11/golgi-apparatus-steel-industry-of-the-simple-cell-%E2%80%93-part-6/
  • 10. Mitochondria- animals Chloroplast - plant Mitochondria = organelles that produce energy ATP • Both plants and animals have mitochondria, but plants produce ATP in their chloroplasts. • signaling, • cellular differentiation, • control of cell cycle; cell growth and death, • Mitochondria have a double membrane • Outer is relatively smooth; Inner is very convoluted • The membrane proteins are part of cellular respiration and the synthesis of compounds, they are energy dense with ATP adenosintriphosphate). 10
  • 11. Mitochondria- animals Chloroplast - plant 11 http://en.wikipedia.org/wiki/Mitochondrion
  • 12. Chloroplast - plant Mitrochondria – animal Chloroplasts are organelles • found in plant cells • conduct photosynthesis , that is the chemical process that converts carbon dioxide into organic compounds, especially sugars, using the energy from sunlight. • capture light energy to conserve free energy in the form of ATP. • are green because they contain the chlorophyll pigment. • are members of a class of organelles known as plastids. 12
  • 13. Chloroplast - plant Mitrochondria – animal 13 http://www.tutorvista.com/biology/chloroplasts
  • 14. Lysosome - animal Vacuoles – plant • Lysosomes are organelles • They contain acid hydrolase enzymes. hydrolase is a digestive enzyme that catalyzes the hydrolysis of a chemical bond (breaks down polymers). • Lysosomes break down waste materials and cellular debris. • Lysosomes also digest food particles, and engulfed viruses or bacteria. • The membrane around a lysosome allows the digestive enzymes to work at the 4.5 pH they require. 14 http://www.biology4kids.com/files/cell_lysosome.html
  • 15. Lysosome - animal Phagocytosis Lysosome digesting food 15 http://apbio-werle.wikispaces.com/Cell+and+Cell+Transport+%28Ch+6-7%29
  • 16. Vacuoles - plant Lysosomes – animals • Vacuoles are large, liquid-filled organelles found only in plant cells, occupying up to 90% of a cell's volume and have a single membrane. Vacuole • Their main function is as a space-filler in the cell but they also perform digestive functions similar to lysosomes. 16 http://www.biologycorner.com/APbiology/cellular/notes_cells2.html
  • 17. Plasma membrane = Cell membrane • The cell membrane or plasma membrane is a biological membrane that separates the interior of all cells from the outside environment. • Cell membrane is semi-permeable (selectively permeable) to ions and organic molecules and controls the movement of substances in and out of cells. • It consists of the lipid bilayer with embedded proteins. • Cell membranes serve as the attachment surface for the cell wall, glycocalyx, and intracellular cytoskeleton. 17
  • 18. Plasma membrane = Cell membrane 18
  • 19. Cytoskeleton • The cytoskeleton (CSK) is a cellular "skeleton" made out of protein. It is within a cell's cytoplasm. • Eukaryotic cells contain 3 main kinds of cytoskeletal filaments: – microfilaments, – intermediate filaments – microtubules. 19 http://www.sparknotes.com/biology/cellstructure/intracellularcomponents/section1.rhtml
  • 20. 20
  • 21. Bacteria Cell Prokaryote 21 http://en.wikipedia.org/wiki/Bacterial_cell_structure
  • 22. Bacteria • Bacteria are divided into two major groups: Gram-negative and Gram-positive • Gram-negative bacteria – Inner membrane – Thin layer of peptidoglycan in periplasmic space – Outer membrane = lipopolysaccharide + protein 22
  • 23. Bacteria • Bacteria are divided into two major groups: Gram-negative and Gram-positive • Bacteria come in 4 basic shapes: – Baccilli (rod ) – Cocci (spherical) – Spirilli (spiral) – Vibrios (comma) 23
  • 24. Bacteria - Shapes 24 http://www.biotopics.co.uk/microbes/bacter.html
  • 25. Bacteria – Gram Staining • Gram-negative bacteria – Inner membrane – Thin layer of peptidoglycan in periplasmic space – Outer membrane = lipopolysaccharide + protein • Gram-positive bacteria – Inner membrane – Thick layer of peptidoglycan called 'wall' (causes staining) – No outer membrane (usually) 25
  • 26. Bacteria – Gram Staining 26 http://www.biologie.uni-hamburg.de/b-online/library/micro229/terry/images/micro/gramposneg.gif
  • 27. Bacteria – Gram Staining • Steps in Gram Staining – heat fix a smear of bacterial culture – apply a primary stain of crystal violet – add Gram’s iodine solution to bind crystal violet in the cell – rapid decolorization with alcohol or acetone Gram-positive cells are stained deep purple. – counterstain with safranin Gram-negative cells are stained red. 27
  • 28. Bacteria – Gram Staining 28 http://pathmicro.med.sc.edu/fox/culture.htm
  • 29. Bacteria – Gram Staining • Reagents of Gram Staining – Crystal violet (primary stain) – Gram's Iodine solution (mordant that fixes crystal violet to cell wall) – Decolorizer (e.g. ethanol) – Safranin (secondary stain) – Water (preferably in a squirt bottle) 29
  • 30. Bacteria – Gram Staining • How Gram Staining Works – 2. Gram's iodine solution (iodine and potassium iodide) is added to form a complex between the crystal violet and iodine. This complex is a larger molecule than the original crystal violet stain and iodine and is insoluble in water. – 3. decolorizer dehydrates the peptidoglycan layer, shrinking and tightening it. The large crystal violet-iodine complex is trapped in the thick peptidoglycan layer of Gram positive bacteria and stained. – Conversely, the thin peptidoglycan layer of Gram negative cells cannot hold the crystal violet-iodine complex and the color (stain) is washed out and lost. 30
  • 31. Virus Virus particles (virions) •genetic material (DNA or RNA segment) •capsid (protein coat that protects these genes) •envelope (of lipids) (some virions) Flu virus http://www.abc.net.au/health/library/stories/2004/07/08/1831345.htm 31 http://www.synapses.co.uk/science/fluvirus.html
  • 32. Virus - Bacteriophage • The Capsid – Head. It is protein coat around nucleic acid. Capsid is divided into subunits called capsomeres. • The Body - Rod shaped structure has retractible sheath around a central hollow core. • The Tails – At end of core is spiked plate with 6 tail fibers which help anchor the virus to its host. 32 http://www.armageddononline.org/viruses.html