1. Dr. Alvin Fox Nutrition, Growth and Metabolism

2. KEY TERMS Obligate aerobe Obligate anaerobe Aerotolerant anaerobe Facultative anaerobe Microaerophilic Siderophore Mesophile Thermophile Psychrophile Generation time Growth curve Glycolysis Fermentation Anaerobic respiration Aerobic respiration Tricarboxylic acid (TCA) cycle or Krebs cycle Oxidative phosphorylation Ubiquinone Glyoxylate pathway

12. Siderophores (S) Fe 2+ / S Receptor Fe 2+ / S

13. Measuring bacterial mass (live + dead) in liquid culture Turbidity (Cloudiness)

14. Measuring viable bacteria colony Colony forming units

15. Growth Curve COLONY FORMING UNITS TIME Lag Log Stationary Death

16. Growth Curve TURBIDITY (cloudiness) TIME Lag Log Stationary Autolysis

20. Glycolysis NAD NADH Glucose Pyruvate C6 C3 ADP ATP

21. Fermentation Pyruvate (C3) NADH NAD Short chain alcohols , fatty acids (C2-C4)

22. Anaerobic Respiration = Glycolysis + Fermentation NAD NADH NADH NAD ATP

23. Krebs Cycle (C4-C6 intermediate compounds ) Pyruvate 3CO 2 (C3) NAD NADH NADH NAD Oxidative phosphorylation O 2 H 2 O ADP ATP (C1)

26. The Krebs cycle Citrate Isocitrate Alpha-keto glutarate Succinate Fumarate Malate Oxaloacetate Pyruvate -CO 2 Acetate + -CO 2 NADH -CO 2 NADH C2 C C C4 X x C6

27. Krebs Cycle - sugar as sole carbon source Pyruvate Acetate -CO 2 C4 Pyruvate + CO 2 + Citrate C C3 Oxalo acetate Oxaloacetate -2CO 2 Aspartic acid Krebs cycle ENERGY STORAGE BIOSYNTHESIS C3 C C2 C6 C4 Oxalo acetate X

28. Krebs Cycle – fatty acids as sole carbon source Fatty acids Acetate + Citrate Oxalo acetate -2CO 2 Aspartic acid Krebs cycle ENERGY BIOSYNTHESIS Isocitrate Succinate Glyoxylate + Acetate + Malate Oxalo acetate x C4 C2 C2 C4 C6 -2CO 2

29. The Glyoxylate and Krebs cycles Citrate Isocitrate Alpha-keto glutarate Succinate Fumarate Glyoxylate Acetate Malate Oxaloacetate 1 2