BIOS 275:KERI SHEA: ISGRIGG

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ks994605

Bios 275 Final Project

Technologie

Southern Ohio Habitats

Bios 275
Keri Shea
Spring 2009

Female Red-Winged Blackbird
Agelaius phoeniceus

Male Red-Winged Blackbird
Agelaius phoeniceus

Strouds Run State Park
Mature
Deciduous
Forest

Adult White Tail Deer Print
Odocoileus virginianus

Longtail Salamander
Eurycea longicaudata

River Clam
Seen on Hocking River bed
mr.f_stop flickr.com

Mallard
Seen on Hocking River
annkelliott flickr.com

Fish head
River Roadkill?
Seen on Hocking River bank

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Achillea millefolium (Yarrow)has been used as a medicinal herb for thousands of years. There is historical evidence that many cultures including the Greeks, Native Americans, and Dutch have used yarrow medicinally. In the United States, there has been a recent resurgence in the use of essential oils and other homeopathic treatments. Many companies claim that some essential oils, including yarrow, have antimicrobial properties. Our study tested the antimicrobial capabilities of four commercially produced yarrow extracts against four bacterial strains. We used the Kirby Bauer Disk Susceptibility Test on two Gram negative bacteria species, Pseudomonas aeruginosa and Escherichia coli, and two Gram positive species, Bacillus cereus and Staphylococcus aureus. Distilled water was used as a control. Each species was treated with all four oils and the control with three replicates of each treatment. After 24 hour incubation, we measured the zones of inhibition and used paired t-tests to determine if the effects differed significantly among the treatments and the species. All four purchased oils showed significant growth inhibition on B. cereus. Two of the oils showed significant zones of inhibition on S. aureus and E. coli when compared with controls. P. aeruginosa, which is known for having very high antibiotic resistance,showed no measurable zones of inhibition for any of the four oils. In the future, we plan to use steam distillation to prepare yarrow extracts from local plant material and test this known oil in the manner described above.

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