The Honolulu Technique is one of three cloning methods that can produce live cloned offspring from adult animal cells. It was developed by scientists at the University of Hawaii who successfully cloned mice over five generations. The technique involves removing the nucleus from a donor cell, transferring it into an egg, stimulating the egg to divide, and implanting the cloned embryo into a surrogate mother. While it is more successful than other cloning methods and may help treat diseases, it is also expensive, reduces genetic diversity, and requires many attempts due to mutations.

1. Honolulu Cloning Technique
By: Annie Reich
Block: 4

2. What is the Honolulu Technique?
• Cloning: when multiple identical copies of a DNA sequence
are made. Cloning is also used to create an organism from a
clone cell.
• Honolulu Technique:
o one of three ways of cloning
o produce live, healthy, cloned male or female offspring
from the fibroblast cells of adult animals

3. Facts
• Developed by Teruhiko Wakayama and Ryuzo
Yanagimachi at the University of Hawaii.
• Scientists have successfully produced five
generations of healthy cloned mice.
• Scientist believe that in the future Honolulu
Technique can be used to treat AIDS, cancer,
multiple sclerosis, and also diabetes.
• 3 out of every 100 attempts are successful.

4. Steps of Honolulu Technique
• Step 1:
– A cell’s nucleus is removed from the organism being
cloned.
• Step 2:
– Next a donor nucleus is transferred into an egg.

5. • Step 3:
– Then the egg is treated with special chemicals and taken
care of until it is able to be placed into the surrogate
mother.
• Step 4:
– An identical cloned baby is made.
• Step 5:
– Process is repeated over and over again in
order to make more copies.

6. Pros
• Creates exact copies
• more successful than the Roslin Technique
• Scientist believe this technique can be used to treat AIDS,
cancer, multiple sclerosis, and also diabetes.
• Scientist can test this technique to broaden their
understandings and learn more useful ideas on ways to use
the technique

7. Cons
• Expensive
• Reduces genetic diversity
• Created many mutations before it was successful
• If a virus gets into the mix it can knock out a
whole generation of clones

8. Bibliography
http://biology.about.com/od/biotechnologycloning/ss/cloning-clinic_2.htm
http://library.thinkquest.org/24355/data/details/techniques/honolulu.html
http://www.humancloning.org/threeways.htm
http://robby.nstemp.com/shopping_page.html
http://library.thinkquest.org/24355/data/details/media/honoluluanim.html