1. Methodology and Project Planning STR1 Kawashima

2. Methodology

3. Isolation of Hydrocarbon-degrading Bacteria from Guimaras Soil Samples Soil samples from various oil-contaminated areas in Guimaras will be obtained and stored in 50 mL conical tubes at room temperature. To isolate possible hydrocarbon-degrading bacteria from each sample, a pinch of soil will be inoculated into 50 mL of liquid minimal medium (for 1 L: 0.5g KH 2 PO 4 , 2.0g KNO 3 , 1.0g MgSO 4 7H 2 O, 0.0012g CaCl 2 •2H 2 O, 0.002g FeSO 4 •7H 2 O) with 50 μL of bunker oil (Petron Corporation, Philippines), and subjected to incubation while shaking at 37ºC at 225 rpm for one week. Once a visible change in turbidity is observed, an applicator stick will be dipped into the suspension culture and streak plated on Luria-Burtani (LB) agar (Pronadisa, Madrid, Spain). One streak plate will be made for each soil sample and incubated overnight at 37°C. Isolated bacterial colonies of different colonial morphologies will be picked and subcultured into separate LB agar slants.

4. Isolation of Hydrocarbon-degrading Bacteria from Guimaras Soil Samples Soil samples from various oil-contaminated areas in Guimaras will be obtained and stored in 50 mL conical tubes at room temperature. To isolate possible hydrocarbon-degrading bacteria from each sample, a pinch of soil will be inoculated into 50 mL of liquid minimal medium (for 1 L: 0.5g KH 2 PO 4 , 2.0g KNO 3 , 1.0g MgSO 4 7H 2 O, 0.0012g CaCl 2 •2H 2 O, 0.002g FeSO 4 •7H 2 O) with 50 μL of bunker oil (Petron Corporation, Philippines), and subjected to incubation while shaking at 37ºC at 225 rpm for one week. Once a visible change in turbidity is observed, an applicator stick will be dipped into the suspension culture and streak plated on Luria-Burtani (LB) agar (Pronadisa, Madrid, Spain). One streak plate will be made for each soil sample and incubated overnight at 37°C. Isolated bacterial colonies of different colonial morphologies will be picked and subcultured into separate LB agar slants.

5. Project Management

6. Project Management Tools

7. Process Flowchart

8. GENERAL description of an activity Each activity is bounded in a RECTANGLE ARROWS indicate what’s next

9. Gantt Chart 1 4 6 0 17:00 20:00 21:00 16:00 Time, hours 18:00 19:00 22:00 2 3 5 current Go to Trinoma Get tickets Buy snacks Watch movie Eat dinner Go home

10. 1 4 6 0 17:00 20:00 21:00 16:00 Time, hours 18:00 19:00 22:00 2 3 5 current Names are in imperative form Time increments run along the bottom, in hours/days/weeks/months Task names take up one row Go to Trinoma Get tickets Buy snacks Watch movie Eat dinner Go home

11. 1 4 6 0 17:00 20:00 21:00 16:00 Time, hours 18:00 19:00 22:00 2 3 5 current Bars represent expected duration of the task Arrows connect prerequisite tasks Shaded bars mean finished tasks while unshaded bars are unfinished Shading tells how complete a task is (think percentage) Go to Trinoma Get tickets Buy snacks Watch movie Eat dinner Go home

12. 1 4 6 0 17:00 20:00 21:00 16:00 Time, hours 18:00 19:00 22:00 2 3 5 current This line marks the current time/date Fully shaded bars left of the line are completed tasks Unshaded bars right of the line are tasks yet to be started Unshaded bars LEFT of the line are behind schedule Go to Trinoma Get tickets Buy snacks Watch movie Eat dinner Go home

13. Network Chart A=1 B=2 C=1 D=2 E=1 F=1 Code Description Duration (hr) A Go to Trinoma 1 B Get tickets 2 C Buy snacks 1 D Watch movie 2 E Eat dinner 1 F Go home 1