Kaumil Hplc

Basics of - HPLC ,[object Object],[object Object]

Index ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

What is HPLC ? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Applications of HPLC ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Types of HPLC ,[object Object],[object Object],[object Object],[object Object],[object Object]

Separation based on selection of column material

HPLC instrumentation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Detector Mobile phase Pump Injection Valve Separation column => => => =>

Solvent system ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Pumping system ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Reciprocating pump : Single piston pump

Reciprocating pump : Double piston pump

Sample injection system ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Column ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Detectors ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Types of HPLC detectors Ability to ionize analyte Low LOD; analyte identification Mass Spec Non-specific; high LOD Commercially available Electrochemical Non-specific; interference from solvent Uniform response; 5ng/25  L LOD Scattering Temperature sensitive; high LOD Works w/ nearly all molecules Refractive Index Many solvents IR active Works w/all molecules IR Not everything fluoresces Very specific; low LOD Fluorescence High LOD Works for all wavelengths DAD Non-specific; complex samples; absorption wavelength Works w/all molecules UV-Vis Name Advantage Disadvantage

UV-VIS detector. ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

ELSD (Evaporative Light Scattering Detector)

P9 RP- HPLC Salient features. ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Typical P9 RP-HPLC peak (Agilent-1200).

Kaumil Hplc

Empfohlen

Empfohlen

Weitere ähnliche Inhalte

Was ist angesagt?

Was ist angesagt? (20)

Andere mochten auch

Andere mochten auch (20)

Ähnlich wie Kaumil Hplc

Ähnlich wie Kaumil Hplc (20)

Kürzlich hochgeladen

Kürzlich hochgeladen (20)

Kaumil Hplc