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BIOTECHNOLOGY
  What is the difference between CLONING and DNA CLONING?

CLONING: Production of
identical copies of an organism
through asexual means




DNA CLONING (or Gene Cloning)
Production of many identical copies of a
single gene.
USES FOR GENE CLONING

1. Produce a large quantity of the gene’s product = Protein.
    Example: humulin or human insulin
2. Learn about the function of the protein coded by the
gene
    Example: Role of a particular protein in cancer
protection
3. Make a transgenic organism
   Example: Pest resistant corn
  So do you think a transgenic animal is the
  same as a cloned animal?
BIOTECHNOLOGY
           DNA FINGERPRINTING or PROFILING

What are the 4 steps of fingerprinting?

    1. Collect DNA
    2. Amplify DNA if necessary using Polymerase
    Chain Reaction
    3. Cut DNA in fragments at specific locations

    4. Run an electrophoresis gel
EXERCISE 1
              INVESTIGATE THE ROBBERY
A woman has been attacked in her house. Her husband finds her
and calls the police. She doesn’t remember her attacker. The police
arrests a suspect.

                      STEP 1: Collect Blood

          1. From the woman
          2. From the husband
          3. From the suspect
STEPS 2 and 3: Prepare DNA samples by PCR and
            digestion (amplify and cut)


Crime      Crime     Victim’s   Husband’s Suspect’s
scene      scene      blood       blood    blood
blood       hair
STEP 4: Run an electrophoresis gel
Has DNA a POSITIVE or NEGATIVE charge?              NEGATIVE

 Crime scene Crime           Victim’s       Husband’s    Suspect’s
    blood   scene hair        blood           blood       blood



                  Holes in which the cut DNA is placed




                              gel
Let’s switch the current on…
Crime scene Crime       Victim’s   Husband’s   Suspect’s
   blood   scene hair    blood       blood      blood
Whose blood from the crime scene belongs to?
                    THE WOMAN
 Crime scene Crime     Victim’s Husband’s   Suspect’s
    blood   scene hair  blood     blood      blood
Whose hair from the crime scene belongs to?
                  THE HUSBAND
Crime scene Crime     Victim’s Husband’s   Suspect’s
   blood   scene hair  blood     blood      blood
Does the police have DNA evidence for the suspect?
                            NO
   Crime scene Crime       Victim’s   Husband’s   Suspect’s
      blood   scene hair    blood       blood      blood
EXERCISE 2
                          THE WILL
Marc was married to Julia. They had 2 children: Luke and Maria.
Marc has died from a heart attack. He left a will.
Robert comes in, introduces himself as Marc’s son and wants his
share.
                     STEP 1: Collect Blood
         1.   From Marc
         2.   From Julia
         3.   From Luke
         4.   From Maria
         5.   From Robert
STEPS 2 and 3: Prepare DNA samples by PCR and
           digestion (amplify and cut)


Marc       Julia     Luke      Maria    Robert
STEP 4: Run an electrophoresis gel
Has DNA a POSITIVE or NEGATIVE charge?              NEGATIVE

    Marc        Julia          Luke            Maria      Robert




                  Holes in which the cut DNA is placed




                              gel
Let’s switch the current on…
Marc     Julia    Luke    Maria   Robert
Is Luke Marc’s son?
         Is Luke Julia’s son?
Marc   Julia    Luke     Maria   Robert
Is Maria Marc’s daughter?
        Is Maria Julia’s daughter?
Marc     Julia    Luke     Maria     Robert
Is Robert Marc’s son?
        Is Robert Julia’s son?
Marc   Julia    Luke     Maria   Robert
HUMAN BODY ORGANIZATION

  WHAT ARE THE DIFFERENT LEVELS OF ORGANIZATION?


What is the smallest level?          A cell

What is called a group of cells      A tissue example?
performing a similar function?
What is called a group of tissues?   An organ example?

Organs are organized into?           An organic system
                                     example?

What is the largest level?           The organism
What is a tissue?
A tissue is composed of similarly specialized
cells that perform a common function in the
body
What are the 4 types of tissues?

                    1.   Epithelial
                    2.   Connective
                    3.   Muscular
                    4.   Nervous
What is the function of Epithelial tissue?


1. covers body surfaces and lines cavities
2. supports and binds body parts
3. moves the body and its parts
4. receives stimuli, processes that information,
   and conducts impulses


                    1
What is the function of Nervous tissue?


1. covers body surfaces and lines cavities
2. supports and binds body parts
3. moves the body and its parts
4. receives stimuli, processes that information,
   and conducts impulses


                    4
What is the function of muscle tissue?


1. covers body surfaces and lines cavities
2. supports and binds body parts
3. moves the body and its parts
4. receives stimuli, processes that information,
   and conducts impulses


                    3
What is the function of Connective tissue?


1. covers body surfaces and lines cavities
2. supports and binds body parts
3. moves the body and its parts
4. receives stimuli, processes that information,
   and conducts impulses


                    2
The only major type of tissue specialized for
movement is the___________tissue.

           1.Nervous
           2.cardiac muscle
           3.Muscle
           4.connective
How is Epithelial Tissue Classified?
                   1. Shape of the cells
                   2. Number of layers
What shapes can you find?     Squamous
                              • (squashed) flat

                              Cuboidal
                              • Cube shape

                              Columnar
                              • Column shape



How many layers of cells?     Simple
                              • Only one layer

                              Stratified
                              • Several layers

                              Pseudostratified
                              • Looks stratified but really is simple
What kind of epithelial tissue is this?

                      1. Squamous
                      2. Cuboidal
                      3. Columnar

                      A. simple
                      B. stratified
                      C. pseudostratified
What kind of epithelial tissue is this?

                      1. Squamous
                      2. Cuboidal
                      3. Columnar

                      A. simple
                      B. stratified
                      C. pseudostratified
What kind of epithelial tissue is this?

                      1. Squamous
                      2. Cuboidal
                      3. Columnar

                      A. simple
                      B. stratified
                      C. pseudostratified
Epithelium that appears layered due to the varying levels
at which nuclei are found in cells, but in reality is not
layered, is:

           1. Simple cuboidal epithelium
           2. pseudostratified columnar epithelium
           3. stratified squamous epithelium
           4. stratified columnar epithelium
The primary purpose of stratification, or layering, in
epithelial tissue is for increased: (where could you
find this type of epithelial tissue?)

          1. Protection
          2. Secretion
          3. absorption
The structure of connective tissue correlates with
its function.




   Where do you find Connective tissue?
Loose connective tissue
                            (under the skin)




                            Blood




                        Fibrous connective Tissue
                        (tendons)

Bone   Cartilage
       (at the end of the
       bone)


                                    Adipose Tissue
                                       Fat droplets
Which of these is NOT a connective tissue?
           1. Blood
           2. Bone
           3. Muscle
           4. cartilage
Choosing from the following list of connective tissues,
which one consists of cells in a fluid matrix?

               1. loose connective tissue
               2. adipose tissue
               3. Bone
               4. blood
How many types of Muscle tissues there is?

                    3 types
 What are they?
             Cardiac muscle
             • Not striated
             • In the heart



             Skeletal muscle
             • Striated (lines)
             • In muscle attached to skeleton
             • VOLUNTARY


             Smooth muscle
             • Not striated (no lines)
             • In blood vessel walls, digestive tract
             • UNVOLUNTARY
WHERE DO YOU FIND SMOOTH MUSCLE?


        1. Intestinal Wall

        2. Heart

        3. Blood vessels

        4. Biceps
WHERE DO YOU FIND CARDIAC MUSCLE?


        1. Intestinal Wall

        2. Heart

        3. Blood vessels

        4. Biceps
WHERE DO YOU FIND SKELETAL MUSCLE?


        1. Intestinal Wall

        2. Heart

        3. Blood vessels

        4. biceps
Only Skeletal muscle is voluntary.

            TRUE
            FALSE
What is the function of Nervous tissue?


1. covers body surfaces and lines cavities
2. supports and binds body parts
3. moves the body and its parts
4. receives stimuli, processes that information,
   and conducts impulses
What is the basic unit of the nervous system?

                NEURON
Where do you find nervous tissue?


                       Brain


                       Spinal
                       Cord
CAVITIES          Cranial
                            cavity
                                          Dorsal
                                          cavity
                            Vertebral
                            canal
          Thoracic cavity
                            diaphragm


Ventral
cavity                      spinal cord
              Abdominal
              cavity
                            vertebrae

           Pelvic cavity
Which of these are located in the thoracic cavity?

                 1. Lungs
                                      1 and 5
                 2. Stomach
                 3. Intestine
                 4. Ovaries
                 5. Heart
                 6. Rectum
                 7. Liver
                 8. Brain
                 9. Spinal Cord
Which of these are located in the pelvic cavity?

                 1. Lungs
                 2. Stomach
                                      4 and 6
                 3. Intestine
                 4. Ovaries
                 5. Heart
                 6. Rectum
                 7. Liver
                 8. Brain
                 9. Spinal Cord
Which of these are located in the Abdominal cavity?

                1. Lungs
                2. Stomach
                3. Intestine
                4. Ovaries
                5. Heart            2, 3 and 7
                6. Rectum
                7. Liver
                8. Brain
                9. Spinal Cord
Which of these are located in the cranial cavity?

                 1. Lungs
                 2. Stomach
                 3. Intestine
                 4. Ovaries
                 5. Heart
                 6. Rectum
                 7. Liver
                 8. Brain                8
                 9. Spinal Cord
Which of these are located in the vertebral cavity?

                 1. Lungs
                 2. Stomach
                 3. Intestine
                 4. Ovaries
                 5. Heart
                                         9
                 6. Rectum
                 7. Liver
                 8. Brain
                 9. Spinal Cord
THE SKELETAL SYSTEM
WHAT ARE THE FUNCTIONS OF THE
      SKELETAL SYSTEM?
1.   Supports body
2.   Protects soft body parts
3.   Produces blood cells
4.   Stores mineral and fat
5.   Along with muscles, permits flexible
     body movement
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.


 Axial skeleton           Skull:
Appendicular skeleton    cranium

                         mandible                                                                            clavicle
         Thoracic cage:                                                                                       scapula
                      sternum
                           ribs                                                                        humerus
            Costal cartilages
              Vertebral column
                                                                                                             ulna
                  Pelvic girdle                                                                                 radius
                         sacrum                                                                              carpals
                         coccyx                                                                                metacarpals
                                                                                                              phalanges
                           femur
                           patella
                          fibula
                             tibia
                           tarsals
                    metatarsals
                      phalanges
Which of these is found in the axial skeleton?


              1. Sternum
              2. Carpals            1, 3, 4 and 5
              3. Ribs
              4. Vertebra
              5. Cranium
              6. Patella
              7. Tibia
              8. Femur
Which of these is found in the appendicular skeleton?


              1. Sternum
              2. Carpals            2, 6, 7 and 8
              3. Ribs
              4. Vertebra
              5. Cranium
              6. Patella
              7. Tibia
              8. Femur
What are the 3 types of skeletal joints?

       Don’t move - FIBROUS
       • skull



       Move a little bit -
       CARTILOGENOUS
       • Ribs, sternum , vertebra



       Move - SYNOVIAL
What are synovial joints and what are the different
  types?
          What kind of movement can you do?


permit movement in all   permit movement in   permit only
planes                   one direction only   rotational
                                              movement
Give an example of a ball and socket joint.
Give an example of a hinge joint.
Give an example of a pivot joint.
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.


                    cartilage

                 spongy bone
                                                                                           epiphysis
  (contains red bone marrow)

              compact bone



                  Central cavity
(contains yellow bone marrow)

                                                                                           diaphysis
   Fibrous connective tissue
               blood vessel



                                                                                           epiphysis
epiphysis   Where is the yellow
            bone marrow located?
              In the diaphysis


              What does the yellow
diaphysis     bone marrow store?
            1. Fat
            2. Red blood cells

epiphysis
epiphysis   Where is the red bone
            marrow located?
              In the epiphysis


              What does the red bone
diaphysis     marrow produce?
             1. Fat
             2. Red blood cells

epiphysis
THE MUSCULAR SYSTEM

• Pull on bones to produce movements

• Skeletal muscles = voluntary movements

• Attached to the bone by tendons

• 650 human skeletal muscles
Name the muscle




ABDUCTOR sorry about the
mistake in class
Name the muscle
Name the muscle
Name the muscle
Name the muscle
Name the muscle
Name the muscle

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Practice exam 4

  • 1. BIOTECHNOLOGY What is the difference between CLONING and DNA CLONING? CLONING: Production of identical copies of an organism through asexual means DNA CLONING (or Gene Cloning) Production of many identical copies of a single gene.
  • 2. USES FOR GENE CLONING 1. Produce a large quantity of the gene’s product = Protein. Example: humulin or human insulin 2. Learn about the function of the protein coded by the gene Example: Role of a particular protein in cancer protection 3. Make a transgenic organism Example: Pest resistant corn So do you think a transgenic animal is the same as a cloned animal?
  • 3. BIOTECHNOLOGY DNA FINGERPRINTING or PROFILING What are the 4 steps of fingerprinting? 1. Collect DNA 2. Amplify DNA if necessary using Polymerase Chain Reaction 3. Cut DNA in fragments at specific locations 4. Run an electrophoresis gel
  • 4. EXERCISE 1 INVESTIGATE THE ROBBERY A woman has been attacked in her house. Her husband finds her and calls the police. She doesn’t remember her attacker. The police arrests a suspect. STEP 1: Collect Blood 1. From the woman 2. From the husband 3. From the suspect
  • 5. STEPS 2 and 3: Prepare DNA samples by PCR and digestion (amplify and cut) Crime Crime Victim’s Husband’s Suspect’s scene scene blood blood blood blood hair
  • 6. STEP 4: Run an electrophoresis gel Has DNA a POSITIVE or NEGATIVE charge? NEGATIVE Crime scene Crime Victim’s Husband’s Suspect’s blood scene hair blood blood blood Holes in which the cut DNA is placed gel
  • 7. Let’s switch the current on… Crime scene Crime Victim’s Husband’s Suspect’s blood scene hair blood blood blood
  • 8. Whose blood from the crime scene belongs to? THE WOMAN Crime scene Crime Victim’s Husband’s Suspect’s blood scene hair blood blood blood
  • 9. Whose hair from the crime scene belongs to? THE HUSBAND Crime scene Crime Victim’s Husband’s Suspect’s blood scene hair blood blood blood
  • 10. Does the police have DNA evidence for the suspect? NO Crime scene Crime Victim’s Husband’s Suspect’s blood scene hair blood blood blood
  • 11. EXERCISE 2 THE WILL Marc was married to Julia. They had 2 children: Luke and Maria. Marc has died from a heart attack. He left a will. Robert comes in, introduces himself as Marc’s son and wants his share. STEP 1: Collect Blood 1. From Marc 2. From Julia 3. From Luke 4. From Maria 5. From Robert
  • 12. STEPS 2 and 3: Prepare DNA samples by PCR and digestion (amplify and cut) Marc Julia Luke Maria Robert
  • 13. STEP 4: Run an electrophoresis gel Has DNA a POSITIVE or NEGATIVE charge? NEGATIVE Marc Julia Luke Maria Robert Holes in which the cut DNA is placed gel
  • 14. Let’s switch the current on… Marc Julia Luke Maria Robert
  • 15. Is Luke Marc’s son? Is Luke Julia’s son? Marc Julia Luke Maria Robert
  • 16. Is Maria Marc’s daughter? Is Maria Julia’s daughter? Marc Julia Luke Maria Robert
  • 17. Is Robert Marc’s son? Is Robert Julia’s son? Marc Julia Luke Maria Robert
  • 18. HUMAN BODY ORGANIZATION WHAT ARE THE DIFFERENT LEVELS OF ORGANIZATION? What is the smallest level? A cell What is called a group of cells A tissue example? performing a similar function? What is called a group of tissues? An organ example? Organs are organized into? An organic system example? What is the largest level? The organism
  • 19. What is a tissue? A tissue is composed of similarly specialized cells that perform a common function in the body What are the 4 types of tissues? 1. Epithelial 2. Connective 3. Muscular 4. Nervous
  • 20. What is the function of Epithelial tissue? 1. covers body surfaces and lines cavities 2. supports and binds body parts 3. moves the body and its parts 4. receives stimuli, processes that information, and conducts impulses 1
  • 21. What is the function of Nervous tissue? 1. covers body surfaces and lines cavities 2. supports and binds body parts 3. moves the body and its parts 4. receives stimuli, processes that information, and conducts impulses 4
  • 22. What is the function of muscle tissue? 1. covers body surfaces and lines cavities 2. supports and binds body parts 3. moves the body and its parts 4. receives stimuli, processes that information, and conducts impulses 3
  • 23. What is the function of Connective tissue? 1. covers body surfaces and lines cavities 2. supports and binds body parts 3. moves the body and its parts 4. receives stimuli, processes that information, and conducts impulses 2
  • 24. The only major type of tissue specialized for movement is the___________tissue. 1.Nervous 2.cardiac muscle 3.Muscle 4.connective
  • 25. How is Epithelial Tissue Classified? 1. Shape of the cells 2. Number of layers What shapes can you find? Squamous • (squashed) flat Cuboidal • Cube shape Columnar • Column shape How many layers of cells? Simple • Only one layer Stratified • Several layers Pseudostratified • Looks stratified but really is simple
  • 26. What kind of epithelial tissue is this? 1. Squamous 2. Cuboidal 3. Columnar A. simple B. stratified C. pseudostratified
  • 27. What kind of epithelial tissue is this? 1. Squamous 2. Cuboidal 3. Columnar A. simple B. stratified C. pseudostratified
  • 28. What kind of epithelial tissue is this? 1. Squamous 2. Cuboidal 3. Columnar A. simple B. stratified C. pseudostratified
  • 29. Epithelium that appears layered due to the varying levels at which nuclei are found in cells, but in reality is not layered, is: 1. Simple cuboidal epithelium 2. pseudostratified columnar epithelium 3. stratified squamous epithelium 4. stratified columnar epithelium
  • 30. The primary purpose of stratification, or layering, in epithelial tissue is for increased: (where could you find this type of epithelial tissue?) 1. Protection 2. Secretion 3. absorption
  • 31. The structure of connective tissue correlates with its function. Where do you find Connective tissue?
  • 32. Loose connective tissue (under the skin) Blood Fibrous connective Tissue (tendons) Bone Cartilage (at the end of the bone) Adipose Tissue Fat droplets
  • 33. Which of these is NOT a connective tissue? 1. Blood 2. Bone 3. Muscle 4. cartilage
  • 34. Choosing from the following list of connective tissues, which one consists of cells in a fluid matrix? 1. loose connective tissue 2. adipose tissue 3. Bone 4. blood
  • 35. How many types of Muscle tissues there is? 3 types What are they? Cardiac muscle • Not striated • In the heart Skeletal muscle • Striated (lines) • In muscle attached to skeleton • VOLUNTARY Smooth muscle • Not striated (no lines) • In blood vessel walls, digestive tract • UNVOLUNTARY
  • 36. WHERE DO YOU FIND SMOOTH MUSCLE? 1. Intestinal Wall 2. Heart 3. Blood vessels 4. Biceps
  • 37. WHERE DO YOU FIND CARDIAC MUSCLE? 1. Intestinal Wall 2. Heart 3. Blood vessels 4. Biceps
  • 38. WHERE DO YOU FIND SKELETAL MUSCLE? 1. Intestinal Wall 2. Heart 3. Blood vessels 4. biceps
  • 39. Only Skeletal muscle is voluntary. TRUE FALSE
  • 40. What is the function of Nervous tissue? 1. covers body surfaces and lines cavities 2. supports and binds body parts 3. moves the body and its parts 4. receives stimuli, processes that information, and conducts impulses
  • 41. What is the basic unit of the nervous system? NEURON Where do you find nervous tissue? Brain Spinal Cord
  • 42. CAVITIES Cranial cavity Dorsal cavity Vertebral canal Thoracic cavity diaphragm Ventral cavity spinal cord Abdominal cavity vertebrae Pelvic cavity
  • 43. Which of these are located in the thoracic cavity? 1. Lungs 1 and 5 2. Stomach 3. Intestine 4. Ovaries 5. Heart 6. Rectum 7. Liver 8. Brain 9. Spinal Cord
  • 44. Which of these are located in the pelvic cavity? 1. Lungs 2. Stomach 4 and 6 3. Intestine 4. Ovaries 5. Heart 6. Rectum 7. Liver 8. Brain 9. Spinal Cord
  • 45. Which of these are located in the Abdominal cavity? 1. Lungs 2. Stomach 3. Intestine 4. Ovaries 5. Heart 2, 3 and 7 6. Rectum 7. Liver 8. Brain 9. Spinal Cord
  • 46. Which of these are located in the cranial cavity? 1. Lungs 2. Stomach 3. Intestine 4. Ovaries 5. Heart 6. Rectum 7. Liver 8. Brain 8 9. Spinal Cord
  • 47. Which of these are located in the vertebral cavity? 1. Lungs 2. Stomach 3. Intestine 4. Ovaries 5. Heart 9 6. Rectum 7. Liver 8. Brain 9. Spinal Cord
  • 49. WHAT ARE THE FUNCTIONS OF THE SKELETAL SYSTEM? 1. Supports body 2. Protects soft body parts 3. Produces blood cells 4. Stores mineral and fat 5. Along with muscles, permits flexible body movement
  • 50. Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Axial skeleton Skull: Appendicular skeleton cranium mandible clavicle Thoracic cage: scapula sternum ribs humerus Costal cartilages Vertebral column ulna Pelvic girdle radius sacrum carpals coccyx metacarpals phalanges femur patella fibula tibia tarsals metatarsals phalanges
  • 51. Which of these is found in the axial skeleton? 1. Sternum 2. Carpals 1, 3, 4 and 5 3. Ribs 4. Vertebra 5. Cranium 6. Patella 7. Tibia 8. Femur
  • 52. Which of these is found in the appendicular skeleton? 1. Sternum 2. Carpals 2, 6, 7 and 8 3. Ribs 4. Vertebra 5. Cranium 6. Patella 7. Tibia 8. Femur
  • 53. What are the 3 types of skeletal joints? Don’t move - FIBROUS • skull Move a little bit - CARTILOGENOUS • Ribs, sternum , vertebra Move - SYNOVIAL
  • 54. What are synovial joints and what are the different types? What kind of movement can you do? permit movement in all permit movement in permit only planes one direction only rotational movement
  • 55. Give an example of a ball and socket joint.
  • 56. Give an example of a hinge joint.
  • 57. Give an example of a pivot joint.
  • 58. Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. cartilage spongy bone epiphysis (contains red bone marrow) compact bone Central cavity (contains yellow bone marrow) diaphysis Fibrous connective tissue blood vessel epiphysis
  • 59. epiphysis Where is the yellow bone marrow located? In the diaphysis What does the yellow diaphysis bone marrow store? 1. Fat 2. Red blood cells epiphysis
  • 60. epiphysis Where is the red bone marrow located? In the epiphysis What does the red bone diaphysis marrow produce? 1. Fat 2. Red blood cells epiphysis
  • 61. THE MUSCULAR SYSTEM • Pull on bones to produce movements • Skeletal muscles = voluntary movements • Attached to the bone by tendons • 650 human skeletal muscles
  • 62. Name the muscle ABDUCTOR sorry about the mistake in class