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Principles of collection and submission of culture specimens to Microbiology lab Dr Kamran Afzal FCPS Microbiology
Introduction  ,[object Object],[object Object],[object Object],[object Object]
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Gen considerations - collection ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
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Blood Culture Specimens ,[object Object],[object Object],[object Object],[object Object],[object Object]
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Cerebrospinal Fluid Specimens ,[object Object],[object Object],[object Object]
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Pus / Pus Swabs  ,[object Object],[object Object],[object Object],[object Object]
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[object Object],[object Object],[object Object],[object Object],[object Object]
Urine Specimens ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
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Faecal Specimens ,[object Object],[object Object],[object Object]
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Sputum Specimens ,[object Object],[object Object],[object Object],[object Object],[object Object]
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Throat/Nasal Swabs Specimens ,[object Object],[object Object],[object Object],[object Object]
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[object Object],[object Object],[object Object],[object Object],[object Object],Ear Specimens
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Eye Specimens
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Dental / Oral Specimens ,[object Object],[object Object],[object Object]
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General considerations – Transportation  ,[object Object],[object Object],[object Object],[object Object],[object Object]
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Transport Media  ,[object Object],[object Object],[object Object],[object Object]
Cary-Blair Tpt Medium ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Amies Tpt Medium ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Stuart Tpt Medium ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
APW ,[object Object],[object Object],[object Object],[object Object]
VTM ,[object Object],[object Object],[object Object],[object Object]
Cultures That Should Include a  Gram Stain   ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Criteria For Rejection of Microbiological Specimens
 
Tables
Principle #1:  The specimen must be collected with a minimum of contamination as close to  site of infection as possible (cont.)
Principle #2: A  specimen  must be collected at the optimal time(s) in order to  recover the pathogen(s) of interest
Principle #2: A specimen must be collected at the optimal time(s) in order to recover  the pathogen(s) of interest (cont)
Principle #3: A sufficient quantity of the specimen must be obtained to perform the requested tests
Principle #4: Appropriate collection devices and specimen containers must be used to  ensure recovery of all organisms
Principle #4: Appropriate collection devices and specimen containers must be used to  ensure recovery of all organisms
Principle #4: Appropriate collection devices and specimen containers must be used to  ensure recovery of all organisms
Principle #4: Appropriate collection devices and specimen containers must be used to  ensure recovery of all organisms
Principle #4: Appropriate collection devices and specimen containers must be used to  ensure recovery of all organisms (Cont)
Suggested Transport Media – General Comments
Principle #5: Collect all microbiology test samples prior to the institution of antibiotics
Principle #6: The specimen container must be properly labeled and sealed prior to transport
Principle #7: Minimize transport time or maximize transport media. There is always some  loss of viability during transport  Minimize transport time and maximize use of transport media as much as possible
Environmentally Fragile Organisms  QA monitor??
Principle #8:  Special handling/Collection instruction must be followed
Principle #8:  Special handling/Collection instruction must be followed (Cont.)  ,[object Object],[object Object],[object Object],[object Object]
Principle #9:  Improper specimen Collected for Ordered Test

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Coll Trans Micro Specimens

  • 1. Principles of collection and submission of culture specimens to Microbiology lab Dr Kamran Afzal FCPS Microbiology
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  • 45. Principle #1: The specimen must be collected with a minimum of contamination as close to site of infection as possible (cont.)
  • 46. Principle #2: A specimen must be collected at the optimal time(s) in order to recover the pathogen(s) of interest
  • 47. Principle #2: A specimen must be collected at the optimal time(s) in order to recover the pathogen(s) of interest (cont)
  • 48. Principle #3: A sufficient quantity of the specimen must be obtained to perform the requested tests
  • 49. Principle #4: Appropriate collection devices and specimen containers must be used to ensure recovery of all organisms
  • 50. Principle #4: Appropriate collection devices and specimen containers must be used to ensure recovery of all organisms
  • 51. Principle #4: Appropriate collection devices and specimen containers must be used to ensure recovery of all organisms
  • 52. Principle #4: Appropriate collection devices and specimen containers must be used to ensure recovery of all organisms
  • 53. Principle #4: Appropriate collection devices and specimen containers must be used to ensure recovery of all organisms (Cont)
  • 54. Suggested Transport Media – General Comments
  • 55. Principle #5: Collect all microbiology test samples prior to the institution of antibiotics
  • 56. Principle #6: The specimen container must be properly labeled and sealed prior to transport
  • 57. Principle #7: Minimize transport time or maximize transport media. There is always some loss of viability during transport Minimize transport time and maximize use of transport media as much as possible
  • 59. Principle #8: Special handling/Collection instruction must be followed
  • 60.
  • 61. Principle #9: Improper specimen Collected for Ordered Test

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