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This question rssumes E.coli are carrying the following plasmid: Open the file attached here to answer the following questions. In the space below type your answers to the following questions: 1. Which plate(s) will E.coli that carry this plasmid be able to grow on? (1 mark) a. LB b. LB+Kan+Amp c. LB+GFP d. LB+Kan e. LB+Amp 2. Explain why cells with this plasmid can grow on that plate/ those plates. Be detailed in your explanation to demonstrate your understanding of how antibiotics are used as selectable markers. ( 2 marks) 3. Does the gel show the predicted results for this plasmid if you digested the plasmid with the restriction enzymes indicated? If not, explain which digest result(s) do not align with the prediction. (2 marks) 4. When the student extracted the plasmid DNA, did they extract all supercoiled plasmid? All relaxed circulat/nicked plasmid? Or a combination of both? Explain how you determined this. (2 marks).

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