1. This question rssumes E.coli are carrying the following plasmid:
Open the file attached here to answer the following questions. In the space below type your
answers to the following questions: 1. Which plate(s) will E.coli that carry this plasmid be able
to grow on? (1 mark) a. LB b. LB+Kan+Amp c. LB+GFP d. LB+Kan e. LB+Amp 2. Explain
why cells with this plasmid can grow on that plate/ those plates. Be detailed in your explanation
to demonstrate your understanding of how antibiotics are used as selectable markers. ( 2 marks)
3. Does the gel show the predicted results for this plasmid if you digested the plasmid with the
restriction enzymes indicated? If not, explain which digest result(s) do not align with the
prediction. (2 marks) 4. When the student extracted the plasmid DNA, did they extract all
supercoiled plasmid? All relaxed circulat/nicked plasmid? Or a combination of both? Explain
how you determined this. (2 marks)