Hydrotherm: A Case Study

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Acid hydrolysis refers to a chemical process in which acid is used to convert cellulose or starch to sugar. In this presentation, we will learn how Hydrotherm do an automated acid hydrolysis to perform traditional fat determination.

Technologie Business

Fat Determination Study -
Hydrotherm vs. AOAC 922.06

Nutrient used as an energy source
1 g = 9 calories (38 kilojoules)
Referred to as a lipid

Importance of Fat
Nutrition
Appearance
Texture
Flavor

Fat Determination
FDA mandate for food label
Total Fat, Saturated Fat, Trans Fat, Cholesterol
“Good” and “Bad” fat

Fat Extraction
Extractable fat = “free fat”
Bound fat needs to be extracted
Methods to measure Total Fat (free + bound)
 Weibull-Stoldt
 AOAC 922.06

Calculating Total Fat Content
Sum Parameter
Sample Matrix
Fatty Acid Spectrum

Study Background
Fat extraction of 21 different products
Traditional AOAC 922.06 vs. Hydrotherm/Weibull-Stoldt
Food and Feed samples tested
Over 1,000 data points collected

Test Equipment
Traditional AOAC 922.06
Weibull-Stoldt

Study Results
Chip Samples Test Data
Soup & Sauce Test Data
Meat Test Data
Link
Link
Link

Benefits of
Weibull-Stoldt /Hydrotherm Method
Cost savings
Time savings
Reproducible Results
Improved precision
High through-put
Universal
No fume hood required

Observations
Overall Testing Ease
Cost Savings
Significant Time Savings
Safety Advantages

Conclusions
Weibull-Stoldt/Hydrotherm exceeds AOAC standards
9 of the 21 products had higher fat recoveries
Significant time savings are 7 minutes per sample
22% cost reduction per sample
“The elimination of flammable hazardous waste allows the
Hydrotherm….to be greener and environmentally friendly.”

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