The document discusses glucose isomerization, which is the conversion of glucose to fructose using the enzyme glucose isomerase. Glucose and fructose have different structures but the same chemical formula. Glucose isomerase aids in converting glucose to the sweeter fructose. In an experiment, a packed bed reactor was used to pump glucose solutions through immobilized glucose isomerase enzyme at varying flow rates to observe the conversion to fructose. Data was collected to determine the kinetic parameters Vmax and KM and develop equations to scale up the process industrially.

1. Glucose Isomerization February 28, 2006 Kate Cannady Chris Miller Matt Mobily Jennifer Pratt Separations & Reaction Engineering Lab

8. Michaelis-Menten…in a nutshell Solve for intermediates using SSA Vmax = maximum rate appearance of fructose [G] = concentration of glucose KM = derived constant W = weight of enzyme ρ E = density of the enzyme F GO = flow rate of feed into system Theory Could determine V max & K M from Lineweaver-Burk Plot; intercept = 1/V max , slope = K M /V max + Design equation for PBR Combine equations and integrate to get ‘w’ as a function of K m , V max , F A0 and x Using equation derived for ‘w’, set up Excel spreadsheet to find K M and V max for corresponding F G and x

10. Preliminary Results

13. Questions?