1. A GC chromatograph shows some serious tailing on a peak. You decrease the split ratio and the tailing is no longer a problem. What was the likely cause of the tailing and why did decreasing the split ratio fix it?
2. A GC chromatograph shows some serious tailing on a peak. You increase the split ratio and the tailing is no longer a problem. What was the likely cause of the tailing and why did increasing the split ratio fix it?
1. A GC chromatograph shows some serious tailing on a peak. You decrease the split ratio and the tailing is no longer a problem. What was the likely cause of the tailing and why did decreasing the split ratio fix it?
2. A GC chromatograph shows some serious tailing on a peak. You increase the split ratio and the tailing is no longer a problem. What was the likely cause of the tailing and why did increasing the split ratio fix it?
2. A GC chromatograph shows some serious tailing on a peak. You increase the split ratio and the tailing is no longer a problem. What was the likely cause of the tailing and why did increasing the split ratio fix it?
Solution
1 & 2
Tailing is caused when few places on the stationary phase i.e) point at which solid should get adsorbed actually
retains the solute in higher fractions when compared to other sites in mobile phase which is indeed encouraged
by some extra unwanted column volume disturbing the flow path.
Lower split ratio means lower sample volume present inside the column and vice versa. By decreasing
the split ratio the column overload on the stationary phase side can be reduced or controlled which is the
main cause of tailing. This is why decreasing split ratio will fix the tailing problems most of the times.
.
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1. 1. A GC chromatograph shows some serious tailing on a peak. You decrease the split ratio and
the tailing is no longer a problem. What was the likely cause of the tailing and why did
decreasing the split ratio fix it?
2. A GC chromatograph shows some serious tailing on a peak. You increase the split ratio and
the tailing is no longer a problem. What was the likely cause of the tailing and why did
increasing the split ratio fix it?
1. A GC chromatograph shows some serious tailing on a peak. You decrease the split ratio and
the tailing is no longer a problem. What was the likely cause of the tailing and why did
decreasing the split ratio fix it?
2. A GC chromatograph shows some serious tailing on a peak. You increase the split ratio and
the tailing is no longer a problem. What was the likely cause of the tailing and why did
increasing the split ratio fix it?
2. A GC chromatograph shows some serious tailing on a peak. You increase the split ratio and
the tailing is no longer a problem. What was the likely cause of the tailing and why did
increasing the split ratio fix it?
Solution
1 & 2
Tailing is caused when few places on the stationary phase i.e) point at which solid should get
adsorbed actually
retains the solute in higher fractions when compared to other sites in mobile phase which is
indeed encouraged
by some extra unwanted column volume disturbing the flow path.
Lower split ratio means lower sample volume present inside the column and vice versa. By
decreasing
the split ratio the column overload on the stationary phase side can be reduced or controlled
which is the
main cause of tailing. This is why decreasing split ratio will fix the tailing problems most of the
times.
