الاضداد واللقاحات وطريقة التصبيع

1. Antibodies and vaccines

2. Traditional polyclonal antibody preparations:

passive
-
Antisera
‘immunoglobulin preparations’.
IgG

3. Antisera
immunoglobulin
-
-
Antisera
-
-
-

4. NaCl 0.9% --
0.25%

5. Monoclonal antibodies



7. Antibody screening: phage display technology


E.Coli

9. 


Unique surface antigene

10. Tumour immunology



NK cells



11. 



CEACarcinoembryonic antigen

ricin, pokeweed toxin, Pseudomonas toxin

16. 



α-fetoprotein (AFP) – CEA - CA125

17. Humanization
-

18. Antigen
Binding Site
Variable
Regions
Constant
Regions
Effector
Functions

19. Chimeric mAb

20. Chimeric mAb

21. CHIMERIC HUMAN/MOUSE
FR1 FR2 FR3 FR4
CDR1 CDR2 CDR3
CONSTANT
CONSTANT
CONSTANT
FR1 FR2 FR3 FR4
CDR1 CDR2 CDR3
Mouse
Human
Chimeric

22. Human Mouse
Chimeric

23. HUMANIZED ANTIBODIES
FR1 FR2 FR3 FR4
CDR1 CDR2 CDR3
CONSTANT
CONSTANT
CONSTANT
FR1 FR2 FR3 FR4
CDR1 CDR2 CDR3
Mouse
Human
Humanized

24. Human Mouse
Humanized

25. Vaccination

T
B
--
-
-

26. Passive


-
-
-
-

27. Active
Attenuated
Killed
Purified components “or macromolecules

28. VaccinesAttenuated
Bacillus Calmette-Guerin
Sabin
poliovirus

29. -
–

30. 

31. Killed Vaccines

-
-


32. 

33. 


34. Bacterial Polysaccharide
Capsules
Toxoids
Proteins from
Pathogens Produced by Recombinant Techniques
Synthetic Peptides
Naked Gene
Anti-idiotype vaccines

35. Bacterial Polysaccharide
Capsules

T
TT
conjugate vaccine

36. Toxoids


37. Synthetic Peptides

38. Naked Gene

CpG

gene gun

39. 
-
-
-

42. Adjuvants


-
-A
LPS
AMLA

-T
INF-yTh1
-B