EuFMD OS20

1. COMPARISION OF FMD SEROSURVEILLANCE RESULTS IN AZERBAIJAN
DURING 2016 - 2019
T. Aliyeva*1, J. Aliyev2, A. Hajiyeva1, M. Vatani1, Ch.Suleymanova1
1. Azerbaijan Food Safety Institute,
2. Azerbaijan Food Safety Agency, Baku, Azerbaijan Republic
ABSTRACT
Introduction
One of main components of Risk Based National
Strategy for prevention and control of FMD is
biannual vaccination of large (LR) and small (SR)
ruminants. For estimating of effectiveness of
vaccination campaigns and for the determining the real
situation regarding circulation of virus in the country
there is carry out serosurveillance, detecting the level
of antibodies to nonstructural proteins (NSP-Ab) of
FMD in susceptible animals as well as the evaluation of
immunity level (SP-Ab) of vaccinated animals. This
study aimed to compare of obtained results of the
serosurveillance for 2016 - 2019.
Materials and methods
Samples were collected randomly and calculated by
the WinEpi software by 95% confidence level with 4%
of margin of. Totally of 1131 (LR) and 1136 (SR) serum
samples in 2016, and 1323 (LR) and 1834 (SR) serum
samples in 2019 were analysed in Central Veterinary
Laboratory of Azerbaijan Food Safety Institute for
antibodies against NSP and SP.
Results
For 2016 the NSP-Ab prevalence was 2.63% and
2.56% for LR and SR ruminants respectively. SP-Ab
results were 88.9% (A), 81% (O), 83,8% (Asia-1) for
LR and 77,6% (A), 72,3% (O) for SR. A total of 5,14%
LR and 5,89% SR samples were positive for NSP-Ab in
2019. In case of SP-Ab, the prevalence was higher in
SR (68,2% (A), 66,6% (O)), than in LR (64,3% (A),
62,3% (O), 59, 6% (Asia-1)).
Discussion
Results indicated, that NSP-ab prevalence level
increased from 2016 to 2019. FMDV has circulated in
country without evident clinical signs due to the annual
vaccination of susceptible livestock were carried out.
According to SP-Ab results in 2019, efforts should
concentrate to select more effective vaccine and
strengthening control measures during vaccination
campaign. Local farmers’ and field veterinarians FMD
awareness should be improved (booklets, posters,
trainings, workshops).
INTRODUCTION
MATERIALS AND METHODS
Samples were collected randomly according to
serosurveillance design and calculated by the WinEpi
software by 95% confidence level with 4% of margin of.
Totally of 1131 (LR) and 1136 (SR) serum samples in
2016, 2865 (LR and SR) in 2017, 1323 (LR) and 1834
(SR) serum samples in 2019 were analysed in Central
Veterinary Laboratory of Azerbaijan Food Safety
Institute for antibodies against NSP and SP (Chart 1).
These samples were analyzed for antibodies against
the NSP of FMD virus by ID Screen FMD NSP
Competition ELISA and antibodies against SP the three
FMDV serotypes (O, A, Asia 1) using ID Screen FMD
Type O, A Competition, FMDV serotype Asia 1 IZSLER
Biotechnology Laboratory test kits.
RESULTS
DISCUSSION
CONCLUSIONS
ACKNOWLEDGMENTS
Epizootic analysis of FMD in the region, especially in
neighboring countries, indicates that because of FMD
outbreaks in neighboring countries, this disease remains a
serious problem for Azerbaijan. Obtained laboratory
results indicated, that FMDV has circulated in country, but
risk zone 1 and 3 and 4 is still the main threats to livestock
of Azerbaijan. Taking into account, that Azerbaijan only
imports animals, meat price is more high, than in
neighboring countries, as well as the intense economic ties,
there are many ways for causative agent introduction, the
risk of infection distribution becomes very high.
Analysis of serosurveillance data for 2016, 2017 and 2019
years demonstrated, that NSP-ab prevalence level increased
from 2016 to 2019. FMDV has circulated in country
without evident clinical signs due to the annual vaccination
of susceptible livestock were carried out. According to SP-
Ab results in 2019, efforts should concentrate to select more
effective vaccine and strengthening control measures
during vaccination campaign. Also enhancing of farmer’s
and field veterinarians awareness for early detection of
disease, and strengthening of regional cooperation are
necessary.
 FMDV has circulated in country
 Territories of risk zone 1, 3 and 4 demonstrate the
highest NSP prevalence
 Current measures are adequate, but continuous
vaccination and monitoring are necessary, because
of animal imports to Azerbaijan.
 Efforts should concentrate on areas with highest
levels of NSPAb prevalence
 Selection of more effective vaccine and
strengthening control measures during
vaccination campaign are crucial
 Awareness of local farmers and field veterinarians
regarding FMD disease in high risk zones should
be improved (booklets, posters, trainings,
workshops)
 Joint regional projects for improving cooperation
between neighboring countries regarding FMD
control are necessary
FMD is one of the priority diseases in Azerbaijan’s
National Animal Health Program. Status of the
Azerbaijan Republic has estimated on stage 2 to the
Progressive Control Pathway Roadmap (PCP).
According of the developed National Risk Based
Strategy Plan (RBSP) main FMD control components
are biannual mass vaccination of ruminants and
following postvaccination seromonitorings.
Seromonitoring campaigns carrying out to evaluate the
efficacy of the vaccination program and to estimate
FMDV circulation through detection of the level of
antibodies to structural (SP) and nonstructural (NSP)
proteins.
Based on FMD regional situation and epidemiology analysis
in country, risk zones of virus introduction were identified:
1. Border villages with Iran, Armenia, Georgia
Iran: Astara, Lerik, Yardymly, Jalilabad, Belasuar, Imishly,
Beylaqan, Fizuli
Georgia: Aghstafa, Tovus, Samukh, Gakh, Zaqatala, Balaken
Armenia: Aghdjabedy, Aghdam, Terter ,Goranboy, Gadabey, Qazakh
2. Border villages with Russia: Khachmaz, Qusar
3. Big live animal markets and villages around live animal markets:
In Barda, Beylaqan, Imishly, Aghdash, Baku, Absheron districts
4.Villages through which lead seasonal migration routes and on
pastures
5. Other areas (background)
6. Absheron peninsula
AZERBAIJAN FOOD SAFETY AGENCY
AZERBAIJAN FOOD SAFETY INSTITUTE
FAO EuFMD
RESULTS
Chart 2. NSP prevalence in 2016-2019
Chart 1. Total tested serum samples LR and SR in 2016-2019
Figure 1. Risk zones of FMDV introduction in Azerbaijan
Table 1. SP-Ab results in 2016 and 2019
For 2016 the NSP-Ab prevalence was 2.63% and 2.56% for
LR and SR ruminants respectively. SP-Ab results were
88.9% (A), 81% (O), 83,8% (Asia-1) for LR and 77,6% (A),
72,3% (O) for SR. For 2017 the NSP-Ab prevalence was
3.1% for LR and 2.9% for SR. A total of 5,14% LR and
5,89% SR samples were positive for NSP-Ab in 2019. In
case of SP-Ab, the prevalence was higher in SR (68,2% (A),
66,6% (O)), than in LR (64,3% (A), 62,3% (O), 59, 6%
(Asia-1)) .
Target
animals
Days after
vaccination
(mean)
FMD Type
A PI/titer
average
FMD
Type O
PI/titer
average
FMD Type
Asia -1
PI/titer
average
2016
LR 35 88.9% 81% 83.8%
SR 42 77.6% 72.3%
2019
LR 35 64.26% 62.3% 59.6%
SR 42 68.2% 66.6%
Table 1. Results of NSP survey (2016 - 2019)
1131 1136
2865 2865
1323
1834
0
1000
2000
3000
4000
LR SR
2016 2017 2019
RISK ZONES
2016 2017 2019
Sampl
e size
#of NSP
positive
samples
Prevalenc
e,
%
Sampl
e size
#of NSP
positive
samples
Prevalenc
e,
%
Sampl
e size
#of NSP
positive
samples
Prevalenc
e,
%
1
Border villages
with Iran,
Armenia,
Georgia
LR 192 16 8,33 1085 68 6,3 478 37 7,74
SR 171 14 8,19 1085 66 6,1 669 38 5,68
2 Border villages
with Russia
LR 100 0 0,00 575 0 0 253 2 0,87
SR 70 0 0,00 575 0 0 356 3 0,84
3
Villages around
live animal
markets and big
live animal
markets
LR 425 5 1,17 210 6 2,9 84 9 10,71
SR 328 4 1,21 210 4 1,9 108 14 12,96
4
Villages through
which lead
seasonal
migration routes
and on pastures
LR 89 1 1,12 380 8 2,1 168 11 6,55
SR 107 1 0,93 380 6 1,6 229 26 11,35
5 Other areas
(background)
LR 105 2 1,9 375 7 1,9 225 7 3,11
SR 105 1 0,95 375 8 2,1 312 24 7,69
6 Absheron
peninsula
LR - - - 240 0 0 115 2 1,74
SR - - - 240 0 0 160 3 1,88
0
1
2
3
4
5
6
LR SR
2,63 2,56
3,1
2,9
5,14
5,89
2016 2017 2019