What is and what is not black fungus and how to diagnose short
•Als PPTX, PDF herunterladen•
0 gefällt mir•209 views
Black fungus is in the news for all the wrong reasons
Empfohlen
Weitere ähnliche Inhalte
Was ist angesagt?
Was ist angesagt? (18)
Ähnlich wie What is and what is not black fungus and how to diagnose short
Ähnlich wie What is and what is not black fungus and how to diagnose short (20)
Mehr von PathKind Labs
Mehr von PathKind Labs (20)
Kürzlich hochgeladen
Kürzlich hochgeladen (20)
What is and what is not black fungus and how to diagnose short
- 1. What is & what is Not black fungus ? Prof. Ashok Rattan, MD, MAMS, Common Wealth Fellow, INSA DFG Fellow, SEARO Temporary Advisor, WHO Lab Director (CAREC/PAHO) Advisor: Pathkind Labs Knowledge Forum, R & D and Quality
- 2. Black fungus Melanized (Dematiaceous) fungi • Dematiaceous fungi have high concentration of melanin and melanine like pigments in their cell walls causing a dark pigment • Produce dusky brown to brown pigment in hyphae or conidia cell walls • Both rapidly and slow growing organisms seen • Conidia vary in appearance from single to multi-celled Melanized Hyaline
- 4. Some important black fungi (Dematiaceous) Single cell conidia • Nigrospora • Aureobasidius • Cladosporium • Phoma • Chaetomium Multi-cell Condia • Alternaria • Epicoccum • Pithomyces • Ulocladium • Stemphyllum Pigmented conidia
- 5. Mucor / Aspergillus are Not Black fungi
- 6. Zygomycosis Aspergillosis • Rhizopus • Mucor • Absidia • Others • Aspergillus flavus • A. fumigatus • A. niger • A. terreus
- 7. Fragments of Mucor in blood well Histopathology shows multiple fragments
- 8. Frozen section from Pulmonary mucor infection
- 10. Aspergillus in the lung showing “Y” branching
- 13. When to seek help ? If you have recovered from Covid 19 disease and are now having • 1. Nasal blockage • 2. Blood tinged nasal discharge • 3. Headache • 4. One sided facial pain / swelling / numbness • 5. Toothache, loosening of teeth, discomfort while chewing • 6. Pain in the eye • 7. Swelling of the eye • 8. Double vision
- 16. Appropriate samples • 1. Skin scraping from cutaneous lesions • 2. Nasal discharge • 3. Scraping & aspirate from sinuses • 4. Bronchoalveolar lavage (BAL) • 5. Needle biopsy from pulmonary lesion • 6. Biopsy of involved tissue • 7. Blood culture is not helpful • 8. CSF may be useful
- 17. Initial examination by microscopy 10 – 20 % KOH • Work inside a class 2 biosafety hood • On a clean glass slide place two drops of 20% KoH • With help of sterile needle, transfer clinical material onto it • Spread the material gently with needle • Cover with cover slipe • Heat gently on the underside of the glass slide (optional) • Leave in a moist chamber for 10 to 15 minutes • Examine under low power & then high power
- 18. 10 to 20% KOH Cutaneous Mucor Nasal Mucor
- 19. Aspergillus in KOH, septate hyphae, branching at 45 degrees
- 21. Culture • Dermatophytes grow best between 25 and 30 C • Opportunistic fungi grow best at 30 C • Invasive fungal infection dimorphic fungi grow at 37 C • Use a combination of media • Sabouraud dextrose agar with antibiotics • Brain Heart Infusion Agar • Best in slopes rather than petri dish (to avoid contaminating the lab) • Candida will grow within 48 hours, most by 3 to 4 weeks
- 24. Aspergillus
- 26. Mucor is not a black fungus Maintain high level of suspension in Covid 19 recovered patients who have been treated with Steroids & are diabetic