2. Tinospora cordifolia (family Menispermacea; commonly
known as Guduchi or Giloy), a glabrous climbing shrub,
is widely used in folk and ayurvedic system of medicine
in India since ancient times.
. The whole plant is used for therapeutic purpose.
A variety of constituents used for drug preparation have
been isolated from the plant. They belong to different
classes such as alkaloids, diterpenoids lactones,
glycosides, steroids, sesquiterpenoides, phenolics,
aliphatic compounds, and polysaccharides
3. 1. Collection of Tinospora cordifolia medicinal plant
from the south India. Andselection of plant based on
in-vitro free radical scavenging activity.
2. To standardize wild Tinospora cordifolia plant crude
drug.
3. To analyse the secondary metabolite content of
Tinospora cordifolia wild’micropropagated plants and
callus through HPLC.
4. To evaluate the hypoglycaemic and antioxidant
activity of Tinospora cordifolia
5. To study the interaction of Berberine and proteins
involved in the control of blood glucose level by using
in silico method its in vivo evaluation.
OBJECTIVES:-
4. . In India, various extracts of the plant are used
as a remedy for many diseases and are included
in various polyherbal preparations used for the
treatment of diabetes, hepatitis, etc.
According to the 1918 United States
Dispensatory, the plant has a long history of use
in India as a medicine and in the preparation of a
starch known as Giloe-ka-sat or as
Palo. Tinospora cordifolia and related species
such as Tinospora
5. T. cordifolia has been used in Ayurvedic
preparations for the treatment of various ailments
throughout the centuries.
It is used as a rasayana to improve the immune
system and body resistance against infections.
T. cordifolia is traditionally used for the treatment
of asthma, and the juice is also employed for the
treatment of chronic coughs. In a clinical study,
100% relief was reported from sneezing in 83% of
the patients on treatment with T. cordifolia
6. Tinospora cordifolia contains many different
chemicals that might affect the body. Some of
these chemicals have antioxidant effects. Others
might increase the activity of the body's immune
system. Some chemicals might have activity
against cancer cells in test animals. Most research
has been done in test tubes or in animals. There
isn’t enough information to know the effects of
Tinospora cordifolia in the human body.
7. 1. Selection of plant material
2. Explant Sterilization
3. Culture medium
4. Inoculation of explant
5. Shoot proliferation
6. Rooting of microshoots
8. Elite plant should be healthy and free of symptoms of
disease,pest problems and showed good biomass yield
All parts of the plant have been used as the
source for micropropagation. Shoot tips of healthy
explants have been however used in most cases
The morphogenetic potential of node, internode
and leaf explants of Isabgol was investigated to
develop reliable protocols for shoot regeneration .
9. Surface sterilization is most important step before
inoculation of explant
Standard protocol for sterilization include washing
thoroughly in running tap water for 30 minutes.
Washing with liquid detergent and Tween 20 for 10min
with vigorous shaking. After washing with detergent
explants , again washing with running tap water to
remove any traces of detergent for 30 minutes.
10. washing is done in laminar hood and then explant kept
in 70% alcohol for 30 seconds.
After alcohol dip, surface sterilization of explant with
freshly prepared 0.1% w/v aqueous solution of
Mercuric chloride for 5 minutes.
After Mercuric chloride treatment, thoroughly washing
for 3-4 times with sterile water to remove any traces of
Mercuric chloride.
11. Murashige and Skoog medium with sucrose 3% and
0.8% agar was generally used by the researchers
Growth hormones, 6-benzylaminopurine (BA), 3-
Indolebutyric acid (IBA), kinetin (Kn), Adenine
sulphate were added to the basal medium either singly
or in various combinations.
Autoclaved the media when taken all material in fixed
amount.
12. The explants were further trimmed and extra outer
leaves were removed to make them in suitable sizes by
the researchers
After cutting explants into suitable size (2-
3cm),explants were transferred to culture bottles
containing MS medium with 0.2mg/L BA and 0.2
mg/L IBA.
Inoculated plants then transferred to growth room.
13. Shoot proliferation- In this the explant is being
scratched from the parent plant by means of scalpel and
then is being proliferated in the MS solid and liquid
medias. Then preserved for 28 days.
Rooting of microshoots- 3-4 cm of shoots were being
exuaded from the parent plant and then placed in 2
types of rooting MS media own with hormone and the
other without. 15days culture is being done.
14. All the media were prepared in double distilled
water
, with 0.8% agar for solidification and pH was
adjusted to 5.7-5.8 with 1N NaOH and 1N HCL.
The media was poured in flasks which were
sterilized in autoclave.
The media were sterilized in autoclave at 1210
C at
15 psi for 15 mins. and stored at room temperature
at 25 ± 1O
C for further use.
15. Tinospora cordifolia seems to be safe when used short-
term. The safety of long-term use, more than 8 weeks, is
not known.
Special Precautions & Warnings:
Pregnancy and breast-feeding: Not enough is known
about the use of Tinospora cordifolia during pregnancy and
breast-feeding. Stay on the safe side and avoid use.
Surgery: Tinospora cordifolia might affect blood sugar
levels, so there is a concern that it might interfere with
blood sugar control during and after surgery. Stop taking
Tinospora cordifolia at least 2 weeks before a scheduled
surgery.
