17. Build sequence contigs based on contigs
defined in TPF (Tiling Path File).
Check for orientation consistencies
Select switch points
Instantiate sequence for further analysis
Switch point
Representative chromosome
sequence
25. The human reference assembly is a COMPOSITE of many individuals
The human reference assembly is NOT static
Accession.versions are KEY to data management
When the reference assembly updates:
Your favorite region may have the same SEQUENCE but different COORDINATES
Your favorite region may CHANGE significantly
We have the TOOLS to help!
http://www.ncbi.nlm.nih.gov/variation
Hinweis der Redaktion
Alignments refer to pairs of sequence. Once you know how a pair of sequences go together, you can look at stringing the pairs along into a contig. The contig is essentially the consensus sequence that is produced from the components.To create a contig, we use the steps shown on this slide.What are switch points? As you create the consensus sequence of the contig, the switch points tell you where to stop using the sequence from one component and begin using the sequence from the next.