Presented at ImmInst 2010 conference http://www.kriorus.ru

2. HISTORY
1900s – glycerol properties discovered by
Russian botanist Maximov
1913 – experiments by Russian physicist
Porfiry Bahmetiev on anabiosis and
hypobiosis on larvae
1929 – the main character in Mayakovsky play
“Bedbug” is cryopreserve
1971 – Russian reanimatologist Vladimir
Negovsky discusses “European cryonics
corporation” with Anatol Dolinov (France)
2000s – we catch up

3. OCTOBER 27, 2005
Cryonics case report at our seminar

4. THE BASIC FACTS
Founded 2005, owned by 9 people
15 patients
$10K neuro / $30K full-body
>50% market share in Europe
A for-profit (easier to register and operate in
Russia), but a non-profit in spirit
5+ compensated part-time employees,
many volunteers

5. STAFF
Valerija Pride, General Director
Igor Artyukhov, Science Director
Danila Medvedev, Chairman
Eugenij Shumilov, Doctor/Technician
Alexey Sulaev, Embalmer/Perfusionist
Andrey Shvedko, Engineer/Philosopher
Victor Grebenshikov, Head of Technical
Council

7. EXAMPLE CASE 1
Patient: 80+ year old relative of a
transhumanist
Death is in the hospital during the weekend
(not in Moscow, but in major city)
Delay in notification: 12 hours
We arrive ~20 hrs after death
The body is cooled passively, core
temperature reaches +9C
Additional time spent on formalities (due to
the weekend): ~15 hrs

8. EXAMPLE CASE 1
Permission from the head of the hospital is
obtained to perform cryopreservation (before
the death certificate, so the patient is in custody
of the hospital)
Head of the pathology department and a nurse
assist
Our specialist oversees the procedure, prepares
the solutions
BPS-7 is used as carrier, ramped up glycerol as
cryoprotector
Access to vasculature is established, washout
and perfusion is done manually (up to 50%
glycerol

9. EXAMPLE CASE 1
Cooling is done using water ice and dry ice
vapour, nasal temperature monitored
Solutions are cooled to a few degrees C
During the operation the core temperature is
about +2
The brain had to be removed per relatives’ request
Cooldown to -80C in a dry ice box with no
temperature adjustment (brain submersed in
glycerol)
The brain is visually inspected, there is no ice
formation visible on the surface

10. EXAMPLE CASE 2
Patient: ~25 year old (in Moscow, several
years ago)
Delay in reporting: 5 hours. Additional delay:
10 hours
In that time we had to find a place to perform
perfusion, find a surgeon, assemble the
team, get necessary supplies in place, move
the patient from the hospital, get necessary
permissions
Access and perfusion done by surgeon,
assistance by our specialist. A perfusion
specialist

11. EXAMPLE CASE 2
Whole body perfusion takes ~7 hours
Whole body is preserved in dry ice
No way to ascertain the quality of the perfusion
(venous return not analyzed for glycerol
concentration)
A perfusionist analyzing the recording heavily
criticized the operation
Later cellular studies show good viability in
cryopreserved fibroblasts, sperm, other cells.
Neurons not studied

13. EXAMPLE CASE 3
A 70+ year old relative of a transhumanist
Death in Moscow hospital (intensive care)
Agreement with administration reached pre-
mortem
Notification about death: <1 hour
Access to the morgue in 2-3 hours
Medication including heparin is injected by
our specialist, indirect heart massage is
performed

14. EXAMPLE CASE 3
The body is additionally cooled with water ice
while in morgue
The body is taken out of the hospital before
government death certificate is issued (only
with a medical death certificate) with the
help of a funeral director
Perfusion takes place in the morgue near
KrioRus
An embalmer experienced in doing open-
circuit embalming perfusion (including 5+
cryonics cases) performs the operation

15. EXAMPLE CASE 3
A roller-pump is used with open-circuit
perfusion
The operation is monitored using video-
conferencing by a US cryonicist (Mike
Darwin) who provides additional advice
Two nurses and our specialist assist
Blood return samples are taken
Head is preserved
Cooling is done with manual temperature
control and tracking (in an acetone bath)

17. EXAMPLE CASE 4
An old dog is cryopreserved
The operation takes place in a private
laboratory
A surgeon is performing the operation
A closed-circuit perfusion is used (takes
12+ hours for initial setup)
The operation starts on a live anesthetized
dog
There is some difficulty establishing
access

20. FACILITIES

21. FACILITIES

22. INTERNATIONAL COOPERATION
Can accept neuro and full-body patients
for storage
Can pick up the patients in Europe, Russia,
CIS
no standby/perfusion capability in EU
Are always willing to share information
with local groups (friendships with
groups in Finland, South Africa,
Australia, Hong Kong, etc.)
Will offer EUCRIO standby to our clients

23. INTERNATIONAL COOPERATION

24. QUESTIONS?
www . kriorus . ru
kriorus @ mail.ru
+7-911-KRIORUS
Moscow, Russia