Slides from my literature review.

1. Cytochrome P450 genes in the
Polistes dominula genome
Daniel Standage, Brendel Group Meeting, 19 Nov 2013
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2. Motivation
 Differential expression analysis in paper wasp
 Initially found 123 differentially expressed gene loci
 Manual examination found many CYP genes
 Results pending
 BLAST2GO enrichment test
 HyCGD

3. Cytochrome P450 (CYP)
 Oxidation enzyme
 RH + O2 + NAD(P)H + H+  ROH + H2O + NADP+
 Found in most (all) organisms
 Broad functional spectrum
 “Substrate promiscuity”
 High copy number

4. CYPs in Insects
 1,744 distinct insect CYPs (Feb 2010)
 +/- 100 CYP genes per species common
 CYPs functions in insects
 Degradation of exogenous chemical compounds
 Odorants
 Sex pheromones
 Toxins and xenobiotics
 Synthesis / degradation of endogenous compounds
 Juvenile hormone
 Ecdysone

5. CYPs in social insects
 Functions not well characterized
 CYPs often associated with juvenile hormone (JH)
 JH, ecdysone, and vitellogenin implicated in caste
determination
 DE analysis has identified 3+ CYP genes with caste-
dependent differential expression

6. CYPs in Solenopsis invicta
 Subtractive hybridization to identify DE genes
 Found 3 genes, 2 of which are CYPs
 Developmentally regulated
 Caste-specifically regulated

7. CYPs in H. sjostedti
 Identified by differential display
 Expression induced by JH analogue
 42% AA identity with CYP6X1; named CYP6AM1
 CYP6AM1 repressed after JHA treatment

8. CYPs in R. flavipes
 Goal: sample CYP4 diversity with degenerate PCR
 Identified seven novel CYP4 genes
 JH-induced differentiation influences expression